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PCR and cloning of recombinant growth hormone cDNA from Cholistani cow

By: Maryam Arshad, Tahira Ruby, Mirza Imran Shahzad, Gulnaz Afzal, Muhammad Rafay

Key Words: Recombinant growth hormone cDNA, Cholistani cow, PCR, Cloning.

Int. J. Biosci. 15(5), 382-386, November 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.5.382-386

Certification: ijb 2019 0224 [Generate Certificate]

Abstract

Cholistani cow breed is a zebu (Bos indicus) or one humped breed of cattle being reared by the nomadic pastoralists of Cholistan desert, Pakistan. With an increasing human population and improving living standards, the demand for meat and milk is also increasing. To cope with such conditions in Pakistan, especially in Cholistan, recombinant technology is used. Importance of recombinant growth hormone rGH in milk and meat production bovine growth hormone bGH has been cloned from a number of species like buffalo, sheep, goat, sheep, and horse etc. The present study was carried out to clone bGH from Chlostani cow. For this purpose, total cellular RNA was extracted from the pituitary gland of freshly slaughtered animal, cDNAs were synthesized and amplified with the help of sequence specific primers. The amplified products were confirmed through restriction digestion and finally cloned in Thyamine Adenine T/A cloning vector followed by transformation in E. coli strain DH5ɑ. Positive clones were confirmed by restriction digestion and sequence analysis confirmed clones were stored at -70˚C as glycerol stock. By using cDNA growth hormone, milk production in young cows can be enhanced. More quantity of milk will compensate the needs of growing population.

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PCR and cloning of recombinant growth hormone cDNA from Cholistani cow

Farooq U, Samad HA, Sher F, Asim M, Khan, MA. 2010. Cholistan and Cholistani Breed of Cattle. Pakistan Veterinary Journal 30, 126-130.

Etherton TD, Bauman DE. 1988. Biology of somatotropin in growth and lactation of domestic animal. American physiological society 78, 745-759.

Bauman DE. 1992. Bovine somatotropin review of an emerging animal technology. Journal of Dairy Science 75, 34-36.

Hauser SD, McGrath MFCollier RJ, Krivi GG. 1990. Cloning and in vivo expression of bovine growth hormone receptor mRNA. Molecular and. Cellular Endocrinology 72, 187-200.

Mauro SMZ, Furlan LR, Ferro MIT, Macari M, Ferro JA. 2002. Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young Bulls treated with recombinant bovine somatotropin. Genetic and Molecular Research 1, 327-336.

Ahmad T, Sarwar M. 2002. Effect of bovine somatotropin on the lactational and reproductive performance of lactating dairy cows. Science vision 8, 36-47.

Paul K. 1998. Bovine Growth Hormones. The Ecologist 28, 1-5.

Chomezynski A, Sacci JB. 1987. Total cellular RNA extraction from tissue. Bio. Analytical biochemistry 162, 156-159.

Venugopal T, Mathavan S, Pandian TJ. 2002. Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy. Journal of Biosciences   27, 261–272.

Butt HI, Shahzad MI, Bashir Q, Saqib MAN, Khanum A. 2014. Plasmid based expression and bioactivity evaluation of caprine growth hormone gene cloned from a local Pakistani goat breed, Beetal. International. Journal of Agriculture and Biology 16, 634-638.

Stewart F, Tuffnell PP. 1991. Cloning the cDNA for horse growth hormone and expression in Escherichia coli. Journal of Molecular Endocrinology 6, 189-96.

Liao MJ, Zhu MYZheng X, Zhang ZH, Zhang AJ. 2003. cDNA cloning of growth hormone from giant panda (Ailuropoda melanoleuca) and its expression in Escherichia coli. Comparative Biochemistry and Physiology 135, 109-116.

Saunders MCDeakin JHarrison GA, Curlewis JD. 1998. cDNA cloning of growth hormone from the Brushtail  Possum (Trichosurus vulpecula). General and Comparative Endocrinology 111, 68-75.

Pan FM, Chang WC. 1988. Cloning and sequencing of bullfrog growth hormone complementary DNA. Biochimica et Biophysica Acta   950, 238-242.

Agellon LB, Chen TT. 1986. Rainbow trout growth hormone: molecular cloning of cDNA and expression in Escherichia coli. DNA and cell biology 5, 463-471.

Zamani M, Berenjian A, Hemmati S, Nezafat N, Ghoshoon MB, Dabbagh F, Mohkam M,  Ghasemi Y. 2015. Cloning, expression, and purification of a synthetic human growth hormone in Escherichia coli using response surface methodology.  Mol Biotech 57, 241-250.

Munaretto R, Kuniechick N, Renard G, Nunes JES, Chies JM, Basso LA, Santos DS. 2009. Construction and cloning of the    bovine growth hormone gene and its expression in Escherichia coli cells. X. Salao.  De.  Iniciacao. Científica 20, 117-118.

Hussain A, Iqbal JS, Akhtar MP, Shakoor A, Waheed U. 2014. Genetic and phenotypic trends for milk yield per lactation in sahiwal cattle under arid and semi-arid conditions of Pakistan. Pakistan journal of life sciences 12, 170‐173.

Maryam Arshad, Tahira Ruby, Mirza Imran Shahzad, Gulnaz Afzal, Muhammad Rafay.
PCR and cloning of recombinant growth hormone cDNA from Cholistani cow.
Int. J. Biosci. 15(5), 382-386, November 2019.
https://innspub.net/ijb/pcr-cloning-recombinant-growth-hormone-cdna-cholistani-cow/
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