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Phytochemical analysis and antibacterial activity of Ajuga bracteosa, Bergenia ciliate, and Amaranthus viridis from District Lower Dir Village Maidan Banda of Khyber Pakhtunkhwa Pakistan

By: Shakir Ullah, Inam Ullah, Majid Khan, Muhammad Zamir, Bakht Tarin Khan, Robeena Naz, Mohammad Sohil, Mohsin Ihsan, Fozia Abasi

Key Words: Phytochemical analysis, antibacterial activity, Ajuga bracteosa, Bergenia ciliate, and Amaranthus viridis.

Int. J. Biosci. 14(5), 403-412, May 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.5.403-412

Certification: ijb 2019 0044 [Generate Certificate]

Abstract

In the present research work, both qualitative and quantative phytochemical investigation of methanolic and ethanolic extracts of Ajuga bracteosa, Bergenia ciliate, and Amaranthus viridis and antibacterial activity was carried out. The study was carried out due to their traditional uses because these plants are commonly used for the aliment of saverales disease. For the sub culturing the bacterial isolates Nutrient broth and Nutrient agar were used. For the sensitivity screening Mueller Hinton agar media was used. The antimicrobial assessments of both the ethanol and methanol extracts showed considerable inhibition activities on the selected isolated bacteria with the concentration of 20mg/ ml. Ajuga bracteosa exhibited more beneficial antibacterial activity in methanol extract of the leaves (30-37mm) in diameter. The leaves ethanol extract showed bacterial inhibition between (30-38mm) in diameter. But, Bergenia ciliate, and Amaranthus viridis leaves extracts as well revealed higher antibacterial activity than the stem bark and extracts of roots. In all the plants parts, Saponins and alkaloids were present. In the leaves extracts of Bergenia ciliate, highest amount of saponins constituent (5.10±0.11%) was recorded. In leaves extract alkaloids was present in highest amount 2.98±0.12 %. The values of MIC ranged between 1.25 10 mg/ml. From the present study we have conclude that the selected plants have high amount of phytochemicals due to which these can be used for further study to isolate the compounds for form the their pharmacological activates.

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Phytochemical analysis and antibacterial activity of Ajuga bracteosa, Bergenia ciliate, and Amaranthus viridis from District Lower Dir Village Maidan Banda of Khyber Pakhtunkhwa Pakistan

Adjakple PM, Ahmad S, Watfa M, Olvera, Hernandez U. Inter Digital Patent Holdings Inc., 2018. Method and apparatus for supporting local IP access and selected IP traffic offload. United States Patent and Trademark Office, 9,924,413.

Chung N, Alexander M. 2002. Effect of soil properties on bioavailability and extractability of phenanthrene and atrazine sequestered in soil. Chemosphere 48(1), p 109-115.

Cool born AF, Bolatito B. 2010. Antibacterial and phytochemical evaluation of three medicinal plants. Journal of Natural Products 3, p 27-34.

Dahiru D, Sini JM, John-Africa L. 2006. Antidiarrheal activity of Zizyphus mauritiana root extract in rodents. African journal of biotechnology 5(10).

De Monte llano PRO, De Voss JJ. 2002. Oxidizing species in the mechanism of cytochrome P450. Natural product reports 19(4), p 477-493.

Ferdous MH, Billah AAM, Mehraj H, Taufique T, Uddin AFMJ. 2015. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. Journal of Bioscience and Agriculture Research 3(02), p 87-95.

Gibson LF. 1998. Bacitracin activity and probiotic activity of aero Mona’s media. Journal of applied microbiology 85(S1), p 243S-248S.

Gracelin DHS, Britto A, Kumar BJRP. 2013. Qualitative and quantitative analysis of phytochemicals in five Pteris species. International Journal of Pharmacy and Pharmaceutical Sciences 5(1), p 105-107.

Hasler CM, Blumberg JB. 1999. Phytochemicals: biochemistry and physiology. Introduction. The Journal of nutrition 129(3), p 756S.

Indrayan A, Wysocki MJ, Kumar R, Chawla A. Singh N. 2002. Estimates of the years-of-life-lost due to the top nine causes of death in rural areas of major states in India in 1995. National Medical Journal of India 15(1), p 7-13.

James ND, de Bono JS, Spears MR, Clarke NW, Mason MD, Dearnaley DP, Ritchie AW, Amos CL, Gilson C, Jones RJ, Matheson D, 2017. Abiraterone for prostate cancer not previously treated with hormone therapy. New England Journal of Medicine 377(4), p 338-351.

Krishnaiah D, Sarbatly R, Bono A. 2007. Phytochemical antioxidants for health and medicine a move towards nature. Biotechnology and Molecular Biology Reviews 2(4), p 97-104.

Mahato SB, Sen S. 1997. Advances in Triterpenoid research, 1990–1994. Phytochemistry, 44(7), p 1185-1236.

Meagher RB, McKinney EC, Kandasamy MK, 1999. Is variant dynamics expand and buffer the responses of complex systems: the diverse plant actin gene family. The Plant Cell 11(6), p 995-1005.

Murugan R, Parimelazhagan T. 2014. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn.–An in vitro approach. Journal of King Saud University-Science 26(4), p 267-275.

Pirzada Z, Personick M, Biba M, Gong X, Zhou L, Schafer W, Roussel C, Welch CJ. 2010. Systematic evaluation of new chiral stationary phases for supercritical fluid chromatography using a standard racemate library. Journal of Chromatography A 1217(7), p 1134-1138.

Sahm DF, Washington JA. 1990. Antibacterial susceptibility Test Dilutions Methods: In: Manuals of Clinical Microbiology. Lennette EH.

Shah RK, Yadav RNS. 2015. Qualitative phytochemical analysis and estimation of total phenols and flavonoids in leaf extract of sarcochlamys pulcherrima Wedd. Global Journal of Bioscience and Bio-Technology 4, p 81-84.

Shakar jian MP, Ali MS, Velíšková J, Stanton PK, Heck DE, Velíšek L. 2015. Combined diazepam and MK-801 therapy provides synergistic protection from tetra methylene di sulfa tetra mine-induced tonic clonic seizures and lethality in mice. Neurotoxic ology 48, p 100-108.

Sodipo OA, Akanji MA, Kolawole FB, Odutuga AA. 1991. Saponin is the active antifungal principle in Garcinia kola, heckle seed. Bioscience Biotechnology Research Communications 3, p.171.

Sodipo OA, Akinnyi JA, Ogunbameru JV, 2000. Studies on certain characteristics of extracts of bark of Pausinystalia johimbe and Pausinystalia macroceras (K Schum) Pierre ex Beille. Global Journal of Pure and Applied Sciences 6(1), p 83-88.

Soni J, Ansari U, Sharma D, Soni S. 2011. Predictive data mining for medical diagnosis: An overview of heart disease prediction. International Journal of Computer Applications 17(8), p 43-48.

Tyagia T, Argawak M. 2017. Phytochemical screening and GC-MS analysis of bioactive constituents in the ethanolic extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) solms. Journal of Pharmacognosy and Phytochemistry 6(1), p 195-206.

Verma SP, Armstrong-Altrin JS. 2013. New multi-dimensional diagrams for tectonic discrimination of siliciclastic sediments and their application to Precambrian basins. Chemical Geology 355, p 117-133.

Shakir Ullah, Inam Ullah, Majid Khan, Muhammad Zamir, Bakht Tarin Khan, Robeena Naz, Mohammad Sohil, Mohsin Ihsan, Fozia Abasi.
Phytochemical analysis and antibacterial activity of Ajuga bracteosa, Bergenia ciliate, and Amaranthus viridis from District Lower Dir Village Maidan Banda of Khyber Pakhtunkhwa Pakistan.
Int. J. Biosci. 14(5), 403-412, May 2019.
https://innspub.net/ijb/phytochemical-analysis-antibacterial-activity-ajuga-bracteosa-bergenia-ciliate-amaranthus-viridis-district-lower-dir-village-maidan-banda-khyber-pakhtunkhwa-pakistan/
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