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Prevalence, incidence, severity and morphological characterization among isolates of karnal bunt (Tilletia indica) in Punjab and Khyber Pakhtunkhwa (Pakistan)

By: Aasma, Shahzad Asad, Muhammad Fayyaz, Anjum Munir

Key Words: Tilletia indica, Karnal bunt, Prevalence, Incidence, Morphological characterization.

Int. J. Biosci. 15(2), 106-117, August 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.2.106-117

Certification: ijb 2019 0072 [Generate Certificate]

Abstract

Karnal bunt is a disease of stern concern all over the world and causing economic losses in wheat growing areas of Pakistan. The disease is known to occur since long and is the main constraint in exporting the wheat in Pakistan. Scanty work on this disease is done in the past. So keeping in view its significance a study was designed to ascertain the disease situation in wheat production zones of Punjab and Khyber Pakhtunkhwa. The disease has shown its increasing trend with few available reports and the pathogen became established in the places where previously it was not known. The prevalence was calculated from both physically observed grains and wash test while isolations of the isolates were made only from damaged grains. During 2013-14 wheat season the average Prevalence, incidence and severity of Karnal bunt in Punjab were 4.87%, 0.66, 0-2 and 14.87%, 1.19 %, 0-2 respectively whereas in Khyber Pakhtunkhwa, it was found to be 11.33%, 3.02.0-2  and 15.06%, 3.49%, 0-2 respectively. In the current study detailed work on the morphological characterization of Tilletia indica was done as the scientific information on pathogen morphology is lacking in Pakistan. Variation was observed in the morphology of 49 isolates on the basis of teliospores size, percentage germination, size of filliform and allantiod sporida (secondary sporidia), growth pattern and colony color. Size of teliospores ranged from 53.09µm (PB-55) to 35.23 µm (PB-68). The germination ranged from 78.53 % (PB-50) to 10.82 % (KPK-17). Continuous monitoring is needed to identify the areas which are free from Karnal bunt pathogen.

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Prevalence, incidence, severity and morphological characterization among isolates of karnal bunt (Tilletia indica) in Punjab and Khyber Pakhtunkhwa (Pakistan)

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Aasma, Shahzad Asad, Muhammad Fayyaz, Anjum Munir.
Prevalence, incidence, severity and morphological characterization among isolates of karnal bunt (Tilletia indica) in Punjab and Khyber Pakhtunkhwa (Pakistan).
Int. J. Biosci. 15(2), 106-117, August 2019.
https://innspub.net/ijb/prevalence-incidence-severity-morphological-characterization-among-isolates-karnal-bunt-tilletia-indica-punjab-khyber-pakhtunkhwa-pakistan/
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