International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Prevalence of tuberculosis in Khyber Pakhtunkhwa Pakistan using GeneXpert assay and light microscopy

By: Kiran Farooq, Aurangzeb, Mohibullah Shah, Muhammad Sameem Javed, Adnan Amjad, Waliullah Khan, Rishma, Sahib Gul Afridi

Key Words: Mycobacterium Tuberculosis, Pakistan, GeneXpert, Light Microscopy.

Int. J. Biosci. 15(4), 223-229, October 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.4.223-229

Certification: ijb 2019 0172 [Generate Certificate]

Abstract

Tuberculosis (TB) is a common disease in developing countries including Pakistan which ranks sixth amongst highest tuberculosis reported countries. The present study aimed to determine the frequency of TB in Peshawar, Mardan and Swabi regions of Khyber Pakhtunkhwa province in Pakistan and also to evaluate the comparative performance of GeneXpert and microscopy analysis. In this cross-sectional study363 patients were included from Mardan (270), Nowshera (42) and Swabi (51). An early morning deep cough sputum of about 2ml was collected from each patient. Two aliquots of each sample were prepared one each for the analysis through GeneXpert and ZN staining based light microscopy. Among 363 cases 207 (57%) were males and 156 (43%) were females. The light microscopy method confirmed 198 (55%) while GeneXpert MTB/RIF assay confirmed 234 (64.4%) samples positive for Tuberculosis. The sensitivity of light microscopy was found 84.6% whereas for GeneXpert MTB/RIF it was 98%. Specificity of light microscopy and GeneXpert were found almost the same. Among the reported positive cases, Mardan constituted 74% of the cases while Nowshera and Swabi had 12% and 14% cases, respectively. Moreover, 83% of the cases had family history of tuberculosis. Maximum number of patients (117) was observed in the age group of 26-40 years. Frequency of TB was higher in Mardan than Nowshera and Swabi. GeneXpert method proved to be more efficient and sensitive.

| Views 24 |

Prevalence of tuberculosis in Khyber Pakhtunkhwa Pakistan using GeneXpert assay and light microscopy

Akhtar N, Khan BT, Saeed K, Khan S, Khan J, Ahmad Z. 2014. “Prevalence of Tuberculosis: Current status in Manglawar District Swat, Khyber Pakhtunkhwa, Pakistan. European Academic Research 1(12), 5160-5166.

Alvarez-Uria G, Azcona JM, Midde M, Naik PK, Reddy S, Reddy R. 2012. Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in HIV-Infected Patients. Comparison of LED Fluorescent Microscopy and the Gene Xpert MTB/RIF Assay in a District Hospital in India. Tuberculosis Research and Treatment 2012.

http://dx.doi.org/10.1155/2012/932862.

Ayaz S, Nosheen T. Khan S, Khan SN, Rubab L, Akhtar M. 2012. Pulmonary Tuberculosis: Still Prevalent In Human in Peshawar, Khyber Pakhtunkhwa Pakistan. Pakistan Journal of Life and Social Sciences 10(1), 39-41.

Bassili A, Seita A, Baghdadi S, Alabsi A, Abdilai I, Agboatwalla M, Maamari F, Nasehi M, Nasir H, Soliman S. 2008. Diagnostic and treatment delay in tuberculosis in 7 countries of the Eastern Mediterranean Region. Infectious Diseases in Clinical Practice 16, 23-35.

http://dx.doi.org/10.1097/IPC.0b013c31815d8d61

Bloom BR. 1994. Tuberculosis: pathogenesis, protection, and control, ASM press.

Causse M, Ruiz P, Gutiérrez-Aroca JB, Casal M. 2011. Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis. Journal of Clinical Microbiology 49, 3065–3067.

http://dx.doi.org/10.1128/JCM.00491-11

Gilani S, Khurram M. 2012. Perception of tuberculosis in Pakistan: findings of a nation-wide survey. Journal of Pakistan Medical Association 62(2), 116-120.

Gnokoro, J. 2001. Therapeutic route of tuberculosis patients identified at the CAT of Adjamé. Abidjan, Ivory Coast: Thesis of Medicine, 106.

Guenaoui K, Harir N, Ouardi A. Zeggai S, Sellam F, Bekri F, Touil SC. 2016. Use of GeneXpert Mycobacterium tuberculosis/rifampicin for rapid detection ofrifampicin resistant Mycobacterium tuberculosis strains of clinically suspected multi-drug resistance tuberculosis cases. Annals of Translational Medicine 4(9), 168.

http://dx.doi.org/10.21037/atm.2016.05.09.

Kochi A. 2001. The global tuberculosis situation and the new control strategy of the World Health Organization. Bulletin of the World Health Organization 79, 71-75.

Marlowe EM, Novak-Weekley SM, Cumpio J, Sharp SE, Momeny MA, Babst A, Carlson JS, Kawamura M, Pandori M. 2011. Evaluation of the Cepheid Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis complex in respiratory specimens. Journal of clinical microbiology 49, 1621-1623.

http://dx.doi.org/10.1128/JCM.02214-10

Rajeswari R, Chandrasekaran V, Suhadev M, Sivasubramaniam S, Sudha G, Renu G. 2002. Factors associated with patient and health system delays in the diagnosis of tuberculosis in South India. The International Journal of Tuberculosis and Lung Disease 6, 789-795.

Reichler MR, Reves R, Bur S, Thompson V, Mangura BT, Ford J, Valway SE, Onorato IM. 2002. Evaluation of investigations conducted to detect and prevent transmission of tuberculosis. Journal of American Medical Association 287, 991-995.

http://dx.doi.org/10.1001/jama.287.8.991

Springer DJ, Saini D, Byrnes EJ, Heitman J, Frothingham R. 2013. Development of an aerosol model of cryptococcus reveals humidity as an Important factor affecting the viability of Cryptococcus during aerosolization. PloS one 8(7), e69804. http://dx.doi.org/10.1371/journal.pone.0069804

Tortoli E, Russo C, Piersimoni C. 2012. Clinical validation ofXpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis. European Respiratory Journal 40(2), 442–447.

http://dx.doi.org/10.1183/09031936.00176311

Van Crevel R, Ottenhoff TH, Van Der Meer JW. 2002. Innate immunity to Mycobacterium tuberculosis. Clinical Microbiology Reviews 15, 294-309.

http://dx.doi.org/10.1128/CMR.15.2.294-309.2002

World Health Organization. 2011. Fluorescent light-emitting diode(LED) microscopy for diagnosis of tuberculosis: policy statement.

World Health Organization. 2014. Global tuberculosis report. 2014. Accessed 19 Nov 2015.

http://apps.who.int/iris/bitst ream/10665 /13709 4/1/97892 41564 809_eng.pdf.

World Health Organization. 2016. Global tuberculosis report 2016. Accessed 20 Apr 2017.

http://apps. who.int/medic inedo cs/docum ents/s2309 8en/s2309 8en.pdf.

World Health Organization. 2017. Global tuberculosis report. 2017. Accessed 24 Feb 2018.

http://www. who.int/tb/publicatio ns/globa l_repor t/en/.

World Health Organization. 2018. Tuberculosis: fact sheet. 2018. Accessed 24 Feb 2018.

http://www.who.int/media centr e/facts heets /fs104 /en/.

Zeka AN, Tasbakan S Cavusoglu C. 2011. Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampicin resistance in pulmonary and extrapulmonary specimens. Journal of Clinical Microbiology 49, 4138-4141.

http://dx.doi.org/10.1128/JCM.05434-11

Kiran Farooq, Aurangzeb, Mohibullah Shah, Muhammad Sameem Javed, Adnan Amjad, Waliullah Khan, Rishma, Sahib Gul Afridi.
Prevalence of tuberculosis in Khyber Pakhtunkhwa Pakistan using GeneXpert assay and light microscopy.
Int. J. Biosci. 15(4), 223-229, October 2019.
https://innspub.net/ijb/prevalence-tuberculosis-khyber-pakhtunkhwa-pakistan-using-genexpert-assay-light-microscopy/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update