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Production of exopolysaccharide by bifidobacteria and its viscometric analysis

By: Sham Lal, Nisar Ahmed Kanhar, Pardeep Kumar, Om Parkash, Anwar Hussain Phulpoto, Majid Ali Maitlo, Muzafar Hussain Sirohi, Ameer Ahmed Mirbahar, Abdul Majid Ansari, Safdar Ali Ujjan, Javed Ahmed Ujjan, Majeeda Ruk, Sapna, Hamid B. Ghoddusi

Key Words: Bifidobacteria; Exopolysaccharide; Viscometry.

Int. J. Biosci. 14(5), 315-323, May 2019.


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The exopolysaccharide production by three Bifidobacterium strains was evaluated by optimizing two parameters (temperature and time). In addition, the role of EPS on viscosity of solutions was observed. Bacterial cultures were grown in MRS broth supplemented with 0.5 % (w/v) cysteine HCl in anaerobic conditions. Among the different time (24 h, 48 h and 72 h) and temperature (30ºC, 37ºC and 42ºC) conditions, high EPS production was observed at 42 ºC after 72 h of incubation. At these conditions maximum amount of EPS was produced by Bifidobacterium breve 11815 with the yield of 94.64 ± 0.25 ug/ml, followed by B. longum 11818 and B. animalis ssp. lactis Bb12 with the yield of 90.53 ± 0.34 ug/ml and 58.8 ± 0.25 ug/ml respectively. Viscometric analysis of EPS performed by viscometer showed highest viscosity of milk (23 ± 1.41 cp) by using EPS produced by B. animalis ssp. lactis Bb12. This study suggests that the foods in which bifidobacteria are used as starter culture should be incubated at 42 ºC to obtain maximum probiotic dose and EPS. Finally, EPS produced by B. animalis ssp. lactis Bb12 can be used for reducing syneresis and improving texture and viscosity of food products.

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Production of exopolysaccharide by bifidobacteria and its viscometric analysis

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Sham Lal, Nisar Ahmed Kanhar, Pardeep Kumar, Om Parkash, Anwar Hussain Phulpoto, Majid Ali Maitlo, Muzafar Hussain Sirohi, Ameer Ahmed Mirbahar, Abdul Majid Ansari, Safdar Ali Ujjan, Javed Ahmed Ujjan, Majeeda Ruk, Sapna, Hamid B. Ghoddusi.
Production of exopolysaccharide by bifidobacteria and its viscometric analysis.
Int. J. Biosci. 14(5), 315-323, May 2019.
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