International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Production, optimization, and anti-cancer activity of L- asparaginase of Pleurotus ostreatus under solid state fermentation

By: Mohamed Yasser A. Bedaiwy

Key Words: Pleurotus ostreatus, L- Asparaginase, Solid state fermentation, Cancer cell line.

Int. J. Biosci. 14(2), 251-263, February 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.2.251-263

Certification: ijb 2019 0142 [Generate Certificate]

Abstract

Agro by-products like Wheat straw, Rice straw, Rice brane, Sugar cane bagasse, saw dust, Maize straw, Cumin straw, Corn seeds, Soya bean husks and Cowpea straw that collected from local farms in Gharbia Governorate, Egypt was used as substrates for production of the medically important L-Asparaginase enzyme under solid state fermentation (SSF). An attempt is made in the present study to optimize the production of L-asparaginase production by Pleurotus ostreatus using wheat straw under solid state fermentation (SSF). The maximum specific activity of L-asparaginase was 3.9 unit/mg that was achieved with the following optimized fermentation parameters: incubation period (9 days), initial moisture content (1: 025), pH 6.0, 30oC, supplemented with Tween 80 as surfactant. L-asparaginase was partially purified with 80 % ammonium sulfate saturation and used to test its efficiency against different cancer cell lines. L-asparaginase enzyme performed an Inhibitory activity against colon carcinoma cells, breast carcinoma cells, and mouse myelogenous leukemia carcinoma cells under an experimental conditions where it performed IC50 of  111 ± 5.4, 204 ± 9.8 and 390 ± 16.6 µg/ml respectively. We are working for the large-scale production of L- asparaginase using cheap growing sources through SSF.

| Views 12 |

Production, optimization, and anti-cancer activity of L- asparaginase of Pleurotus ostreatus under solid state fermentation

Abdel-Fattah YR, Olama Z. 2002. L- asparaginase production by Pseudomonas aeruginosa in solid-state culture: evaluation and optimization of culture conditions using factorial designs. Process Biochemistry 38(1), 115-122.

Albanese E, Kafkewitz K. 1978. Effect of medium composition on the growth and asparaginase production of vibrio succinogenes. Applied Environmental Microbiology 36, 25-30

Arima K. 1964. Microbial Enzyme Production. In: Global Impacts of Applied Microbiology, Starr, M.P. (Ed.). John Wiley and Sons, New York, p 279-294.

Balakrishnan K, Pandey A. 1996. Production of biologically active secondary metabolites in solid state fermentation. Journal of Scientific and Industrial Research 55, 365/72.

Balasubramanian K, Ambikapathy V, Panneerselvam A. 2012. Production, isolation, and purification of L-asparaginase from Aspergillus terreus using submerged fermentation. International Journal of Advances in Pharmaceutical Research 3(2), 778 – 783.

Bilgrami KS, Verma RN. 1981. 2nd ed, Vikas Publishing, Pvt. Ltd, 313-315.

Chanakya P, Nagarjun V, Srikanth M. 2011. International Journal of Pharmaceutical Sciences Review and Research 7(2), 189-192.

El-Bessoumy AA, Sarhan M, Manjour J. 2004. Production, isolation and purification of L- asparaginase from Pseudomonas aeruginosa 50071 using solid- state fermentation. Journal of biochemistry and Molcular biology 37(4), 387-393.

Elshafei AM, Hassan MM, AbouZeid MA, Mahmoud DA, Elghonemy DH (2012) Purification, characterization and antitumor activity of L-asparaginase from Penicillium brevicompactum NRC 829. British Microbiology Research 2(3), 158-174.

Gomha SM, Riyadh SM, Mahmmoud EA, Elaasser MM. 2015. Synthesis and Anticancer Activities of Thiazoles, 1,3-Thiazines, and Thiazolidine Using Chitosan-Grafted-Poly(vinylpyridine) as Basic Catalyst. Heterocycles 91(6), 1227-1243.

Hosamani R, Kaliwal BB. 2011. L-asparaginase-an anti-tumor agent production by fusarium equiseti using solid state fermentation. International Journal of Drug 3(2), 88-99.

Imada A, Igarasi S, Nakahama K, Isono M. 1973. Lasparaginase and glutaminase activities of microorganisms. Journal of General Microbiology 76, 85-99.

Imada A, Igarasi S, Nakahama K, Isono M. 1973, Asparaginase and glutaminase activities of microorganisms. Journal of General Microbiology. 76, 85-99.

Jayaramu M, Hemalatha NB, Rajeshwari KG, Siddalingeshwara KG, Mohsin SM, Sunil Dutt PLNSN. 2010. A novel approach for detection, confirmation and optimization of L-asparaginase from Emericella nidulans. Current Pharmaceutical Research 1(1), 20-24.

Kotra SR, Prudvi N, Sada Sai KRA, Mannava KK, Peravali JB, Anmol Kumar, Sambasiva Rao KRS, Pulicherla KK. 2013. Cost effective process for the production of fungal L-asparaginases from Penicillium sps isolated from local soil sample. Mintage Journal of Pharmaceutical and Medical Sciences 2(1), 45-50.

Kumar K, Verma N. 2012. The various sources and application of L-asparaginase. Asian Journal of Biomedical and Pharmaceutical Sciences  3, 197-205.

Kuo LH. 1967. Animal feeding stuffs compositional data of feeds and concentrates (Part 3). Journal of Malaysian Agriculture 46, 63-70.

Lapmak K, Lumyong S, Thongkuntha S, Wongputtisin P, Sardsud U. 2010. Chiang Mai Journal of Science 37, 160–164.

Lonsan BK, Ghildyal NP, Budiatman S, Ramakrishna SV. 1985. Engineering aspects of solid-state fermentation. Enzyme and Microbial Technology 7, 258/65.

McAllister TA, Stanford K, Bae HD, Treacher R, Hristov AN, Baah J, Shelford JA, Cheng KJ. 2000. Effect of a surfactant and exogenous enzymes on digestibility of feed and on growth performance and carcass traits of lambs. Canadian journal of Animal science 80, 35-44.

McKnight S. 2003. Gene switching by metabolic enzymes–how did you get on the invitation list? Cell 114, 150-152.

Mishra A. 2006. Appl. Biochem. Biotechnol, 135, 33-42.

Mohsin SM, Sunil Dutt PLNSN, Siddalingeshwara KG, Karthik J, Jayaramu M, Naveen M, Vishwanatha T, Prathiba KS. 2012. Optimization of fermentation conditions for the biosynthesis of L-Asparaginase by Penicillium sp. Journal of Academia and Industrial Research 1(4), 180-182.

Monica T, Lincoln L,  Niyonzima FN,  Sunil SM. 2013. Isolation, purification and characterization of fungal extracellular L-asparaginase from Mucor Hiemalis. Journal of Biocatalysis and Biotransformation 2(2), 1-9.

Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological. Methods 65, 55-63.

Mostafa SA, Salama MS. 1979. L-asparaginase producing Streptomyces from soil of Kuwait. Zentralbl Bakteriol Naturwiss 134(4), 325–334.

Mudgetti RE, In Demain AL, Soleman NA. 1986. American Society of Nair A, Kumar R, Agalya Devi R. 2013. Screening of commonly available solid process residues as substrate for l-asparaginase production by Aspergillus terreus MTCC 1782. Research Journal of Pharmaceutical, Biological and Chemical Sciences  4, 1731–1737.

Neeta Asthana S, Azmi W. 2003.Microbial L-asparginase: A potent antitumor enzyme. Indian Journal of Biotechnology 2, 184–94.

Radcliffe CW, Kafkewitz D, Abuchowski A .1979. Asparaginase production by human clinical isolates of Vibrio succinogenes. Applied Environmental Microbiology 38, 761-762.

Sreenivasulu V, Jayaveera KN, Mallikarjuna Rao P. 2009. Research Journal of Pharmacognosy and Phytochemistry 1, 30-34.

Upm-Makna. 2010. Cancer research laboratory, Institute of Bioscience.

Usha KY, Praveen 1K, Reddy BR. 2014. Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea. Biotechnology Research International Article ID 815495, p 1-9.

Usman A. 2015. Seeking efficacy in L-asparaginase to combat acute lymphoblastic leukemia (ALL): a review. African Journal of Pharmcy and Pharmacology 9, 793–805.

Venil C, Lakshmanaperumalasamy P. 2009. The Internet Journal of Microbiology 7(1), 10-18.

Mohamed Yasser A. Bedaiwy.
Production, optimization, and anti-cancer activity of L- asparaginase of Pleurotus ostreatus under solid state fermentation.
Int. J. Biosci. 14(2), 251-263, February 2019.
https://innspub.net/ijb/production-optimization-anti-cancer-activity-l-asparaginase-pleurotus-ostreatus-solid-state-fermentation/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update