International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Qualitative and quantitative analysis on colonizing facial bacterial strains among tertiary students of Iligan City

By: Primrose M. Odtojan, Lucilyn L. Maratas

Key Words: Bacterial strains, tertiary students, qualitative analysis.

J. Bio. Env. Sci. 13(4), 166-172, October 2018.


Certification: ijb 2018 0021 [Generate Certificate]


The most prominent skin-covered anatomical region is the human face which is a privileged site for growth of microorganisms. This study was conducted to provide information in establishing healthy baseline of facial microflora. This study involved a convenience sample of 135 healthy tertiary students with no evident skin infections. The skin surface samples were obtained by the swab method and all samples were allowed to grow on nutrient agar to determine colonization rates. All clinical samples exhibited bacterial growth   on nutrient agar   while 87% of the MSA plates had growth of bacterial colonies. Two hundred sixty-seven isolated bacterial colonies were randomly picked from NA and MSA plates and were purified and subjected to different identification techniques – colonial characterization, staining for cellular morphologies and biochemical tests. Through these conventional methods of identification, four bacterial species were identified up the genus level: Staphylococcus remained the predominant genus of the superficial skin microbiota at 39% ( Staphylococcus aureus) and 19% (coagulase negative staphylococci); followed by Micrococcus (2%), and Bacillus sp.(1%).

| Views 56 |

| Views 56 |

Qualitative and quantitative analysis on colonizing facial bacterial strains among tertiary students of Iligan City

Coagulase Test: Principle, procedure and interpretation. 2018.

Cogen AL, Yamasaki K, Sanchez KM, Dorschner RA, Lai Y, MacLeod DT, Torpey JW, Otto M, Nizet V, Kim JE, Gallo RL. 2010. Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin. Journal of Investigative Dermatology 130(1), 192-200.

Cogen AL, Nizet V, Gallo RL. 2008. Skin microbiota: a source of disease or defence? British Journal of Dematology 158(3), 442-455.

Dekio I. 2012. Microorganisms and Atopic Dermatitis, Atopic Dermatitis Jorge Esparza-Gordillo and Itaru Dekio, Intech Open, Available from:

Grice EA, Segre JA. 2011. Skin Microbiome. Nature Reviews Microbiology 9, 244-253.

Gloor M, Peters G, Stoika D. 1982. On the resident aerobic bacterial skin flora in unaffected skin of patients with atopic dermatitis and in healthy controls. Dermatologica 164(4), 258-265.

MacFaddin JF. 2000. Catalase-Peroxidase Tests. Biochemical Tests for Identification of Medical Bacteria. 3rd ed. Philadelphia: Lippincott Williams and Wilkins; 78-97.

Murillo N, Raoult D. 2013. Skin microbiota: overview and role in the skin diseases acne vulgaris and rosacea. Future Microbiology 8(2), 209-222.

Ogawa T, Katsuoka K, Kawano K, NIshiyama S. 1994. Comparative study of staphylococcal flora on the skin surface of atopic dermatitis patients and healthy subjects. Journal of Dermatology 21(7),453-60.

Triple Sugar Iron Agar (TSI): Principle, Procedure and Interpretation. 2018.

Todar K. 2012. The Normal Bacterial Flora of Humans. Todar’s Online Textbook of Bacteriology.

Primrose M. Odtojan, Lucilyn L. Maratas.
Qualitative and quantitative analysis on colonizing facial bacterial strains among tertiary students of Iligan City.
J. Bio. Env. Sci. 13(4), 166-172, October 2018.
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
innspub logo
english language editing
    Publish Your Article
    Submit Your Article
Email Update