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A Study on genetic diversity and evolutionary analysis of ECA1 Protein in different plant families

By: Roohi Aslam, Nasar Virk, Muhammad Qasim Hayat, Atif Shafique

Key Words: P2A type ATPase, P2B type ATPase, N terminal autoinhibitory domain, Clustal W, Endosomal.

Int. J. Biosci. 17(4), 134-143, October 2020.


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Calcium and manganese are two of the most important nutrients required by plants. Calcium/manganese uptake and accumulation is carried out by a large group of transporters. Among these, the P-type ATPases are considered most important. These ATPases have been divided into two categories i.e., P2A and P2B based on the absence and presence of the N terminal autoinhibitory domain respectively. ECA1 is an important P2A-type ATPase that is localized in the endosomal system and has crucial roles in calcium and manganese translocation. In this study, the phylogeny of ECA1 protein within different plant families as well as conserved motifs putatively involved in Ca2+ ions binding was investigated. Phylogenetic analysis and diversity in predicted tertiary structures indicated that ECA1 protein is functionally conserved but structurally diverged in different plant families. Moreover, some amino acids are found to be involved in Ca2+ ions binding in ECA1 proteins of different plant species which gives the idea that the evolution of ECA1 gene is monophyletic hence, indicating divergent evolution.

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A Study on genetic diversity and evolutionary analysis of ECA1 Protein in different plant families

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Roohi Aslam, Nasar Virk, Muhammad Qasim Hayat, Atif Shafique.
A Study on genetic diversity and evolutionary analysis of ECA1 Protein in different plant families.
Int. J. Biosci. 17(4), 134-143, October 2020.
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