International network for natural sciences – research journal
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Use of Black Pepper Seed as Growth Enhancer in Labeo rohita

By: Matiullah, Fayyaz Rasool, Ehsan Mehmood Bhatti, Noor Khan, Shakeela Parveen

Key Words: Black Pepper, Growth enhancer, Labeo rohita, Fish nutrition, proximate analysis.

Int. J. Biosci. 8(3), 1-9, March 2016.


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Nutritionists are continuously in search and use of natural growth promoters for their respective animal feeds because of their use results in no side effects which are imminent by the use of various chemicals. The field of fish nutrition also needs some breakthrough if some natural growth promoter is discovered. Black pepper seed is natural flavor that could be explored as a feed additive to enhance growth, disease resistance and survival in fish. In the present study, we studied the effect of graded levels of black pepper seeds (BPS) on growth performance, feed and nutrient utilization, body composition in Labeo rohita. The trial was conducted in four cemented rectangular tanks. Each tank was further sub-divided into three replicates having dimensions 2.896 x 0.762 x 0.914 (length x width x depth). There were 12 fishes (Labeo rohita) stocked (with pre-recorded their morphometric) in every replicate with average weight of 9.9 grams. The fish feed was formulated, prepared, pelleted and dried having 30% CP level. It was further categorized into experimental feeds on the basis of BPS i.e. 0.0, 0.5, 1.0, and 2.0 % of BPS as feed additive. The feed was dispensed twice a day, six days a week, and for 60 days. The physic-chemical parameters were maintained and recorded on daily basis. Each fish was caught every fortnightly and its weight and length was recorded. Fish fed with 0.5% (BPS) exhibited significantly higher (P<0.05) SGR, feed conversion ratio (FCR), % weight gain. Fish fed 0.5% BPS was had significantly higher (P<0.05) protein, fat, ash, fiber contents. Anyhow, no significant difference (P>0.05%) was found in Phosphorus contents. Therefore, it is recommended to use black pepper seeds in feeds as growth enhancer in fish at commercial scale @ 0.5% BPS.

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