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A cross sectional study on Bacteriological profile and antibiogram of cholecystitis and cholelithiasis

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Research Paper 01/01/2021
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A cross sectional study on Bacteriological profile and antibiogram of cholecystitis and cholelithiasis

Dr Srinivas N, Dr Udayasri B
Int. J. Micro. Myco.12( 1), 1-7, January 2021.
Certificate: IJMM 2021 [Generate Certificate]
Key: Antimicrobial susceptibility testingBacteriological profileBile cultureCholecystitisCholelithiasis

Abstract

Cholecystitis is the most common disease of gastro intestinal tract contributing for 10% disease burden. Most of the time it is infective in origin. In view of the emerging multi drug resistance organisms, there is a need for guidance in empirical antimicrobial therapy in every clinical setting. To study the bacteriological profile and their antimicrobial susceptibility pattern in cholecystitis and cholelithiasis patients. A cross sectional study was conducted at Mamatha General Hospital, Kammam over a period of 2 years from September 2010 to September 2012. A total number of 62 clinically diagnosed cases of cholecystitis and cholelithiasis subjected to elective cholecystectomy were included in the study. Bile and gall stone samples were collected and processed aerobically, anaerobically according to standard microbiological techniques. Antimicrobial susceptibility testing was performed on all isolates by Kirby-bauer disc diffusion method and susceptibility pattern were recorded. A total number of 62 cases of cholecystitis were included in the study shows female preponderance of disease. Maximum number of cases belongs to 41 to 50 years age group. Out of 62 patients 62 bile samples and 58 gall stones specimen were collected and analyzed. Bile culture was positive in 24 cholelithiasis cases (41.37%), Gallstone culture was positive in 9 cases (15.51%). The two bile samples yielded anaerobic growth. Escherichia coli was the predominant organism in both samples. Bacterial isolates showing maximum susceptibility to ampicillin-sulbactum (100%), amikacin (80%). To optimized empirical antimicrobial therapy in cholecystitis and cholelithiasis patients prior knowledge of the prevalence of various bacteria and their antimicrobial susceptibility pattern in is required.

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Abd-Alkareem AY. 2011. Gallstones associated with bacterial incidence. Tikrit Journal of Pure Science 16(4), ISSN: 1813-1662.

Alan A. Bloom, Julian Katz. 2011. Cholecystitis – Medscape reference. Jun 30, emedicine, medscape.Com / article/171886-overview.

Ballal M, Jyothi KN, Antony B, Arun C, Prabhu T, Shivananda PG. 2001. Bacteriological spectrum of cholecystitis and its antibiogram. Indian J Med Microbiol 19, 212-4.

Bergan T, Dobloug I, Liavåg I. 1979. Bacterial Isolates in Cholecystitis and Cholelithiasis – Informa Health Care. Scand J Gastroenterol. July 14(5), 625-631.

Jayanthi V, Palanivelu C, Prasanthi R, Methew S, Srinivasan V. 1998. Composition of gallstones in Coimbatore district of Tamil Nadu State. Ind J Gastroenterol 17, 134-35.

Khurro MS, Mahajan R, Zargar SA, Javid G. 1989. Prevalence of biliary tract disease in India: a sonographic study in adult population in Kashmir. Gut 30, 201-05.

Marne C, Pallares R, Martin R, Sitges-Serra A. 1986. Gangrenous Cholecystitis and Acute Cholangitis associated with Anaerobic Bacteria in Bile. Eur. J. Clin. Microbiol 5(1), 35-39.

Mohan H, Punia RPS, Dhawan SB, Ahal S, Sekhon MS. 2005. Morphological spectrum of gallstone disease in 1100 cholecystectomies in North India. Indian J Surg 67, 140.

Nasir Mahmud Wattoo. 2010. Frequency of infected bile in patients of uncomplicated gallstone disease. Pakistan Armed Forces Medical Journal. June (2).

Ohood Akead Radhi Al-Zuharri. 2001. Isolation and identification of bacteria from patients with cholecystitis and cholelithiasis undergoing cholecystectomy. Kufa university–college of nursing.

Prakash A. 1968. Chronic cholecystitis and cholelithiasis in India. Int Surg 49, 79- 85.

PRL Gomes et al. 2006. Aerobic bacteria associated with symptomatic gallstone disease and their antimicrobial susceptibility. Galle Medical Journal Vol 11, No. 1.

Small DM. 1980. Cholesterol nucleation and growth in gallstone formation. N Engl J Med302, 1305-1311.

Suri A, Yasir M, Kapoor M, Aiman A, Kumar A. 2010. Prospective Study On Biliary Bacteriology In Calcular Disease Of The Gall Bladder And The Role Of Common Newer Antibiotics. The Internet Journal of Surgery 22(2).

Tandon RK. 1988. Pathogenesis of gallstones in India. Trop Gastroenterol 9, 83-91.

Tyagi SP, Tyagi N, Maheshwari V, Ashraf SM, Sahoo P. 1992. Morphological changes in diseased gall bladder: a study of 415 cholecystectomies at Aligarh. J Ind Med Assoc 90, 178-81.

Vasitha Abeysuriya, Kemal Ismil Deen Salgado, Tamara Wijesuriya and Sujatha Senadera Ragama, Sri Lanka. 2008. Microbiology of gallbladder bile in uncomplicated symptomatic cholelithiasis. Hepatobiliary Pancreat Dis Int 7, 633-637.

Willis RG, Lawson WC, Hoare EM, Kingston RD, Sykes PA. 1984. Bile bacteria and sepsis in biliary surgery. Br. J. Surg 71, 845-849.

Vinay DV, Swaroop S, Kelkar R. 1991. Bile microflora and antibiotic sensitivity patterns in malignant obstructive jaundice. Ind J Gastroentero 10, 15-20.

Zafar Iqbal Malik, Manasir Malik, Omer Salahuddin, Muhammad Azhar, Omer Dilawar. 2009. Micro flora of bile aspirates in symptomatic cholelithiasis. Journal of Rawalpindi Medical College 13(1), 38-40.

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