Evaluation of pcr in the molecular diagnosis of trichomonas vaginalis infection in comparison with other conventional methods

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Research Paper 01/08/2016
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Evaluation of pcr in the molecular diagnosis of trichomonas vaginalis infection in comparison with other conventional methods

Evaluation of pcr in the molecular diagnosis of trichomonas vaginalis infection in comparison with other conventional methods
Int. J. Micro. Myco.4( 1), 1-8, August 2016.
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Abstract

Trichomonas vaginalis (T. vaginalis) is a common pathogen with worldwide distribution. It is estimated that worldwide 180 million people are infected annually. Trichomoniasis is associated with vaginitis, cervicitis, low birth weight, and preterm delivery. PCR has the advantage of high sensitivity, shorter time for diagnosis and the ability to detect nonviable or defective organism. In this study we used these three methods for evaluation of PCR in comparison with conventional methods like wet mount and culture in the detection of T. vaginalis in vaginal discharge. Three vaginal swab specimens were obtained from each of 200 cases, of the age group 18-40years, both symptomatic and asymptomatic females attending Gynaecology OPD(50) and Family planning OPD(50) at Gandhi hospital, Secunderabadand two FSW(Female sex workers) clinics (100) in highly concentrated areas of them in Hyderabad, for validation of various forms of Trichomonas vaginalis diagnostic procedures. One swab was immediately examined by wetmount microscopy, a second swab was placed in Wittington’s medium for cultivation, and other swab is placed in 2SP transport medium for PCR for T.vaginalis. A total of 58 samples positive in one or more tests were identified: 11 (5.5%) infections were detected by wet mount microscopy, and 30 (15%) positives in culture respectively. PCR was positive in 50 (25%) samples. PCR appears to be the most sensitive method with high detection rate and method of choice for detection of genital infections with T. vaginalis.

VIEWS 16

Ackers JP. 2003. Trichomonads In: Molecular medical parasitology, Marr JJ, Nilsen TW, Komuniecki RW. 1st Edition. Elsevier Science, Great Britain 137-50.

Adu Sarkodie Y, Opoku BK, Danso KA. 2004. Comparision of Latex Agglutination, Wet preparation and Culture for the detection of Trichomonas vaginalis. Sex Transm Infect 80, 201-203.

Agarawal BM, Sandhya Agrawal Singh & Ghiza Rizvi. PK. 2000. Trichomonas vaginalis AnIndicator for Other Sexually Transmitted Infecting Agents. Indian J Med Microbiol 66, 241-243.

Angelika Stary, Angelika Kuchinka-Koch and Lilianna Teodorowicz. 2002. Detection of Trichomonas Vaginalis on Modified Columbia Agar in the Routine Laboratory. J ClinMicrobiol 40. 3277-3280.

Burstein GR, Zenilman JM. 1999. Nongonococcal urethritisa new paradigm. Clin. Infect. Dis S66-S73.

Charolotte Gaydos, Justin Hardick, Samuel Yang. 2003. Use of the Roche Light Cycler Instrument in a Real – Time PCR for Trichomonas vaginalis in Urine samples from Females and Males. J clinMicrobiol 41, 5619-5622.

Crucitti T, Van Dyck E, Tehe A, Abdellati S, Vuylsteke B, Buve A. Laga. M. 2003. Comparison of culture and different PCR assays for detection of Trichomonas vaginalis in self collected vaginal swab specimens. Sex. Transm. Infect 79:393–398.

Fouts AC, Kraus SJ. 1980. Trichomonas vaginalis: re-evaluation of its clinical presentation and laboratory diagnosis. J. Infect. Dis 141, 137-143.

Gelbart SM, Thomason JL, Osypowski PJ, Kellett AV, James JA, Broekhuizen FF. 1990. Growth of Trichomonas vaginalis in commercial culture media. J. Clin. Microbiol 28, 962-964.

Jane R Schwebke and Lisa Lawing F. 2002. Improved Detection by DNA Amplification of Trichomonas Vaginalis in Males. J clinMicrobiol 40, 3681-3683.

Kazemi B, Yasaee F, Bandehpour M, Seyed N, Mehrabi Y, Rajabnejad M, Mansouri M, Givrad S, Ghazi M, Baseri H. 2004. Diagnosis of Trichomonas infection by urine PCR analysis compared to wet mount microscopic screening. J. Med. Sci 4(3), 206-9.

Kengue P, Veas F, Vidal N, Rey JL, Cuny G. 1994. Trichomonas vaginalis repeated DNA target for highly sensitive and specific Polymerase Chain Reaction diagnosis. Cell. Mol.Biol. (Noisy-Le-Grand) 40, 819-831.

Madico G, Quinn T, Rompalo C, Mckee A, Jr KT, Gaydos CA. 1998. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J. Clin. Microbiol 36, 3205-3210.

Marcia M, Hobbs CorneliaKaydos- Daniels S, William Miller C. 2003. Validation of a Urine-Based PCR- Enzyme-Linked Immunosorbent Assay for use in clinical Research Settings To Detect Trichomonas Vaginalis in Men. J clinMicrobiol 41, 318-323.

Mayta H, Gilman RH, Calderon MM, Gottlifb A, Soto G, Tuero I, Sanchez S, Vivar A. 2000. 18S Ribosomal DNA-based PCR for diagnosis of Trichomonas vaginalis. J. Clin. Microbiol 38(7), 2683-87.

Muresu R, Rubino S, Rizzu P, Baldini A, Colombo M, Cappucinelli P. 1994. A new method for identification of Trichomonas vaginalis by fluorescent DNA in situ hybridization. J. Clin. Microbiol 32,1018-1022.

Patel SR, Wiese W, Patel SC, Ohl C, Byrd JC, Esterada CA. 2000. Systemic review of diagnostic tests for vaginal trichomoniasis. Infect. Dis. Obstet. Gynecol 8, 248-57.

Petrin D, Delgaty K, Bhatt R, Garber G. 1998. Clinical and microbiological aspects of Trichomonas vaginalis. Clin. Microbiol. Rev 11(2), 300-17.

Philips Heine R, Wiesenfeld HC, Sweet R L, Witkin SS. 1997. Polymerase chain reaction analysis of distal vaginal specimens: a less invasive strategy for detection ofTrichomonas vaginalis. J. Infect. Dis 24. 985-987.

Pillay A, Lewis J, Ballard RC. 2004. Evaluation of Xenostrip-TV, a Rapid Diagnostic Test for Trichomonas Vaginalis Infection. J clinMicrobiol 42, 3853-3856.

Rasoul Jamali Rita Zareikar Abdolhasankazemi J. 2006. Diagnosis of Trichomonas vaginalis Infection Using PCR Method Compared to Culture and Wet Mount Microscopy.Int Med 5, 1-15.

Riley DE, Roberts MC, Takayama T, Krieger JN. 1992. Development of a polymerase chain reaction-based diagnosis of Trichomonas vaginalis. J. Clin. Microbiol 30, 465-472.

Rubino S, Muresu R, Rappelli P, Fiori PL, Rizzu P, Erre G, Cappucinelli P. 1991. Molecular probe for identification of Trichomonas vaginalis DNA. J. Clin. Microbiol 29, 702-706.

Ryu JS, Chung HL, Min DY, Cho YH, Ro YS, Kim SR. 1999. Diagnosis of Trichomoniasis by polymerase chain reaction. Yonsei Med. J 40(1), 56-60.

Schmid GP, Matheny LC, Zaidi AA, Kraus SJ. 1989. Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions. J. Clin. Microbiol 27, 1230-1233.

Shaio MF, Lin PR, Liu JY. 1997. Colorimetric one-tube nested PCR for detection of Trichomonas vaginalis in vaginal discharge. J. Clin. Microbiol 35, 132-138.

Swygard H, Seña AC, Hobbs MM, Cohen MS. 2004. Trichomoniasis: clinical manifestations, diagnosis and management. Sex. Transm. Infect 80: 91-5.

Zhang ZF, Graham S, Yu SZ, Marshall J, Zielezny M, Chen YX, Sun M, Tang SL, Liao CS, Xu JL. 1995. Trichomonas vaginalis and cervical cancer. A prospective study in China. Ann. Epidemiol 5: 325–332.