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Influence of carbon and nitrogen sources on the spore yield of Trichoderma harzianum in fed-batch culture

By: Damilola O Seyi-Amole, Abiodun A Onilude

Key Words: Trichoderma harzianum, Spore yield, Carbon sources, Nitrogen sources

Int. J. Micro. Myco. 7(1), 18-23, January 2018.

Certification: ijmm 2018 0011 [Generate Certificate]

Abstract

The influence of carbon and nitrogen sources on the spore yield of T. harzianum in fed-batch culture was investigated. T. harzianum was cultivated in liquid culture media under the effect of different carbon and nitrogen sources at different concentration using a fed-batch process. It was observed that among the various carbon sources studied, glucose (2.5 g l-1) gave the highest spore yield of 2.81±0.14 while starch (15.0 g l-1) gave the lowest spore yield (0.22±0.17). The effect of nitrogen sources revealed that 1.0 g l-1 of casein enhanced the highest spore yield (2.88±0.02) while the lowest spore yield (0.23±0.02) was recorded in medium containing soy meal (9.0 g l-1) preparation. The results revealed that T. harzianum has the ability to utilize various carbon and nitrogen compounds and produce high spore yield at low carbon and nitrogen concentration. This can be considered during industrial production of T. harzianum spores for biocontrol.

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Influence of carbon and nitrogen sources on the spore yield of Trichoderma harzianum in fed-batch culture

Al-Taweil HI, Osman MB, Aidil AH, Yussof WMW. 2009. Optimizing Trichoderma viride Cultivation in Submerged State Fermentation. American Journal of Applied Sciences 6(7), 1277-1281.

Amsellem Z, Zidack NK, Quimby Jr. PC, Gressel J. 1999. Long-term dry preservation of viable mycelia of two mycoherbicidal organisms. Crop Protection 18, 643-649.

Askew DJ, Laing MD. 1994. The in vitro screening of 118 Trichoderma isolates for antagonism to Rhizoctonia solani and an evaluation of different environmental sites of Trichoderma as sources of aggressive strains. Plant Soil 159, 277-281.

Aube C, Gagnon C. 1969. Effect of carbon and Nitrogen nutrition on growth and sporulation of Trichoderma viride pers Ex fries. Canadian journal of Microbiology 15, 703-706.

Baker KF, Cook RJ. 1974. Biological control of Plant Pathogens. Freeman, San Francisco.

Bakri Y, Jacques P, Thonart P. 2003. Xylanase production by Penicillium canescens 10-10c in solid-state fermentation. Applied Biochemistry and Biotechnology 108, 37-748.

Carisse O, Bassam SE, Benhamou N. 2001. Effect of Microsphaeropsis sp. Strain P1 30A on germination and production of sclerotia of Rhizoctonia solani and interaction between the antagonist and the pathogen. Phytopathology 91, 782-791.

Cascino JJ, Harris RF, Smith CS, Andrew JH. 1990. Spore yield and Microcycle Conidiation of Colletotrichum gloeosporioides in Liquid Culture. Applied and Environmental Microbiology 56(8), 2303-2310.

Dorrance AE, Lipps PE, Mills DR. 2001. Rhizoctina Damping-off and Stem Rot of Soybeans. Extension Fact-sheet. Plant Pathology, Columbus, Ohio State University 1-2.

Eric S. 2005. Fungus of the month: Trichoderma. The Environmental Reporter 3, 6.

Gao L, Sun MH, Lui XZ, Che YS. 2007. Effects of carbon concentration and carbon to nitrogen ratio on the growth and sporulation of several biocontrolfungi. Mycological Research 111, 87-92.

Ibrahim YB, Low W. 1993. Potential of Mass Production and Field Efficacy of Isolates of the Entomopathoghenic Fungi Beauveria bassiana and Paecilomyces fumosoroseus on Plutella xylostella. Journal of Invertebrate Patholology 39, 222-232.

Lewis JA, Papavivaz GC. 1983. Production of chlamydospores and conidia by Trichoderma sp. in liquid and solid growth media. Soil BiolBiochem15, 351–357.

Molla AH, Fakhru’l-Razia A, Hanafi MM, Alam MZ. 2004. Optimization of process factors for solid-state bioconversion of domestic wastewater sludge. Int. Biodegrad. 53, 49-55.

Monga D. 2001. Effect of carbon and nitrogen sources on spore germination, biomass production and anti-fungal metabolites by species of Trichoderma and Gliocladium. Indian Phytopathology 54, 435-437.

Nahar S, Hossain F, Feroza B, Halim MA. 2008. Production of glucoamylase by Rhizopus sp. in liquid culture. Pak J Bot 40 (4), 1693–1698.

Onilude AA, Adebayo-Tayo BC, Odeniyi AO, Banjo D. Garuba EO. 2012. Comparative mycelial and spore yield by Trichoderma viride in batch and fed-batch cultures. Ann Microbiol 10, 10-12.

Rai D, Tewari AK. 2016. Evaluation of different carbon and nitrogen sources for better growth and sporulation of T. harzianum (Th14). Journal of Agricultural Biotechnology and Sustainable Development 8(8), 67-70.

Said DD. 2007. Spore Production of Biocontrol Agent Trichoderma harzianum: Effect of C/N ratio and Glucose Concentration. Jurnal Rekayasa Kimia danLingkungan 6(1) 35-40.

Sharma S, Gupta RBL, Yadava CPS. 2002. Selection of a suitable medium for mass multiplication of Entomofungal pathogens. Indian Journal of Entomology 64(3), 254-261.

Shirsole SS, Mishra T. 2014. Effect of nutritional sources on In vitro growth and sporulation of Trichodermaviride. Bioinfolet 11(3b), 940.

Singh B. 2003. Improving the production and utilization of cowpea as food and fodder. Field Crops Research 84, 169–150.

Singh BB, Raj DRM, Dashiel KE, Jackai LEN. 1997. Advances in Cowpea Research. Co- publication of International Institute of Tropical Agriculture (IITA) and Japan International Research Centre for Agricultural Sciences. IITA, Ibadan, Nigeria, Devon 230-320.

Singh SR, Rachie KO. 1985. Cowpea Research, Production and Utilization. John Wiley and Sons, Chrichester, New York 1-7.

Summerfield RJ, Roberts EH. 1985. Grain legume crops. Collins, London 1-6.

Verma M, Satinder KB, Tyagi RD, Surampalli RD, Valer JR. 2005. Wastewater sludge as a potential raw material for antagonistic fungus (Trichoderma sp.): Role of pre-treatment and solids concentration. Water Res 39, 3587–3596.

Waghunde RR, Priya J, Naik BM, Solanky KU, Sabalpara AN. 2010. Optical density – a tool for estimation of spore count of Trichoderma viride. Journal of Biopesticides 3(3), 624-626.

Xiao CL, Sitton JW. 2004. Effects of culture media and environmental factors on mycelial growth and pycnidial production of Potebniamyce spyri. Mycology Research 108, 926-932.

Damilola O Seyi-Amole, Abiodun A Onilude.
Influence of carbon and nitrogen sources on the spore yield of Trichoderma harzianum in fed-batch culture.
Int. J. Micro. Myco. 7(1), 18-23, January 2018.
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