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NDM-1 gene detection from Metallobeta lactamase (MBL) producing Pseudomonas aeruginosa: A pilot study from a teritiary care centre

By: K. Archana Rao, S. Sangeetha, SA. Lakshminarayana

Key Words: NDM-1 gene, Metallobeta lactamase, Pseudomonas aeruginosa, Teritiary care centre

Int. J. Micro. Myco. 10(1), 1-7, July 2019.

Certification: ijmm 2019 0027 [Generate Certificate]

Abstract

Multidrug resistant bacteria always remain a great challenge. The latest threat being New Delhi Metallobetalactamase-1 (NDM-1) a superbug has brought notoriety to Indian Health care. NDM-1 refers to the transmissible genetic element encoding multiple resistant genes, first isolated from a strain of Klebsiella spp. in New Delhi, India, which has the ability to hydrolyse beta lactams and carbapenams. Detection of NDM-1 gene in multidrug resistant Pseudomonas isolates from various clinical samples. 200 Pseudomonas species were isolated in Microbiology laboratory during one year period were included in the study. Samples were processed as per Standard operating procedures. Antibiotic sensitivity testing was done by Kirby-Bauer disc diffusion method. The results were interpreted as per CLSI guidelines. MBL detection was done, by using EDTA Double Disc Synergy Test and Imipenem [I]-EDTA Combined Disc Test. MBL positive isolates were subjected to conventional PCR for genotyping & detection of NDM-1. A cross sectional descriptive study. Out of 12545 samples that were received in microbiology laboratory, 299 Non-Fermenting Gram Negative Bacilli [NFGNB] were isolated of which 200 were speciated as Pseudomonas aeruginosa. 20/200 [10%] were resistant to imipenem and 24/200 [12%] to meropenem. 10% of isolates showed MBL positive. NDM-1 gene was not detected in any of the 20 MBL positive isolates. NDM-1 gene since its origin has caused chaos in the health care facility with its ability to cause various infections. Detection is possible only with molecular methods. Thus gene detection plays a pivotal role in patient treatment and reduction of hospital stay. (Pseudomonas, multidrug resistant, New Delhi Metallobetalactamase-1, super bugs).

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NDM-1 gene detection from Metallobeta lactamase (MBL) producing Pseudomonas aeruginosa: A pilot study from a teritiary care centre

Ahuja AS, Kabra SK. 2002. “Cystic fibrosis: Indian experience.” Ind Pediatr 39, 813-818.

Clinical and Laboratory Standards Institute. 2018. Performance Standards for Antimicrobial Disks Susceptibility Tests; Approved Standard. 10th ed. CLSI document M02-A10. Wayne, PA: CLSI.

Deshpande P, Rodrigues C, Shetty A. 2010. New Delhi Metallo beta lactamase (NDM-1) in Enterobacteriaceae : treatment options with carbapenems compromised. J Assoc Physicians India 58, 147–9.

Falagas ME, Koletsi PK, Bliziotis IA. 2006. The diversity of definitions of multidrug-resistant (MDR) and pandrug-resistant (PDR) Acinetobacter baumannii and Pseudomonas aeruginosa. J Med Microbiol 55(Pt 12), 1619-29.

Fazeli H1, Akbari R, Moghim S, Narimani T, Arabestani MR, Ghoddousi AR. 2012. Pseudomonas aeruginosa infections in patients, hospital means, and personnel’s specimens. J Res Med Sci 17(4), 332-7.

www.cdc.gov/media/releases/2013/p0305_deadly_bacteria.html

www.medicalnewstoday.com/articles/197616.php

Huang CR, Lu CH, Chuang YC, Tsai NW, Chang CC, Chen SF, Wang HC, Chien CC, Chang WN. 2007 “Adult Pseudomonas aeruginosa meningitis: high incidence of underlying medical and/or post neurosurgical conditions and high mortality rate.” Jpn J Infect Dis 60(6), 397-9.

Kashyap A, Gupta R, Sharma R, Verma VV, Gupta S, et al. 2017. New Delhi Metallo Beta Lactamase: Menace and its Challenges. J Mol Genet Med 11, 299 doi:10.4172/1747-0862.1000299

Khan AU, Maryam L, Zarrilli R. 2017. Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): A threat to public health. BMC Microbiol 17, 101.

Lee K, Lim YS, Yong D, Yum JH, Chong Y. 2003. “Evaluation of the Hodge test and the Imipenem-EDTA double-disk synergy test for differentiating of metallo-β- lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter Spp.” J Clin Microbiol 41(10), 4623-9.

Livermore DM, Woodford N. 2000. Carbapenemases: a problem in waiting? Curr. Opin. Microbiol 3, 489-495.

Murray Patrick R, Baron Ellen Jo, Jorgensen James H, Landry Marie Louise. 2003. Manual of clinical Microbiology, Vol I, 8th edition, ASM Press 734-834.

Nordmann P, Poirel L, Carrer A, Toleman MA, Walsh TR. 2011. How to detect NDM-1 producers. J Clin Microbiol 49, 718-21.

Obritsch MD, Fish DN, MacLaren R, Jung R. 2005. Nosocomial infections due to multidrug-resistant Pseudomonas aeruginosa: epidemiology and treatment options. Pharmacotherapy 25(10), 1353-64.

Padhi S. 2011. New Delhi metallo-beta-lactamase: A weapon for the newly emerging drug-resistant bacteria. Indian J Med Sci 65, 317–320.

Pitout JD1, Gregson DB, Poirel L, McClure JA, Le P, Church DL. 2005. Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory. J Clin Microbiol 43(7), 3129-35.

Raghunath D. 2010. New metallo β-lactamase NDM-1. Indian J Med Res 132(5), 478-81.

Rubin SJ, Granato PA, Wasilauskas BL. 1985. Glucose-nonfermenting gram-negative bacteria p. 330-349. In Lennette EH, Balows A, Hausler WJJr, Shadomny HJ (ed.), Manual of clinical microbiology, 4th ed. American Society for Microbiology, Washington, D.C.

Shanthi M, Sekar U, Kamalanathan A, Sekar B. 2014. Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India. Indian J Med Res. 140(4), 546-50.

Washington Winn Jr, Allen Stephen, Janda William, Koneman Elmer, Procop Gary, Screckenberger Paul, Woods Gail. 2006. “The non-fermentative Gram negative bacilli” Koneman’s Colour atlas and textbook of diagnostic Microbiology, 6th edition, Lippincott William & Wilkins 303-392.

Watanabe M, Iyobe S, Inoue M, Mitsuhashi S. 1991. Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother 35, 147–151.

Winn W Jr, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, et al. 2006. editors. In: Koneman’s Color Atlas and textbook of Diagnostic Microbiology. 6th ed. USA: Lippincott Williams and Wilkins Company;. Nonfermenting Gram negative bacilli; pp. 305-91.

Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y. 2002. “Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.” J Clin Microbiol 40(10), 3798-801

Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et al. 2009. Characterization of a New Metallo-β-lactamase gene bla and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother 53, 5046–54.

K. Archana Rao, S. Sangeetha, SA. Lakshminarayana.
NDM-1 gene detection from Metallobeta lactamase (MBL) producing Pseudomonas aeruginosa: A pilot study from a teritiary care centre.
Int. J. Micro. Myco. 10(1), 1-7, July 2019.
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