International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Phytochemical and fungicidal activity of selected plant extracts against Phaeoisariopsis griseola of common bean

By: Simon M. Obila, David M. Musyimi, Phoebe A. Sikuku

Key Words: Botanicals, common bean, concentration, fungi, Phaeoisariopsis griseola

Int. J. Micro. Myco. 11(5), 1-11, May 2020.

Certification: ijmm 2020 0034 [Generate Certificate]

Abstract

Plant extracts are effective in plant pathogens control and are now becoming popular throughout the world. Allium sativum, Azadirachta indica and Tithonia diversifolia extracts have been reported to exhibit antimicrobial activity against phytopathogens. The aim of this study was to phytochemically screen and determine the fungicidal activity of Azadirachta indica, and Tithonia diversifolia extracts against Phaeoisariopsis griseola of common bean. Phytochemical analysis of the plants and pathogenicity test of Phaeoisariopsis griseola were carried out. Phytochemical screening revealed that alkaloids, tannins, terpenoids, saponins, cardiac glycosides and sterols were present in Azadirachta indica, and Allium sativum while sterols, saponins and alkaloids were absent in Tithonia diversifolia. Flavonoids were present in Allium sativum but absent in Azadirachta indica. All the plant extracts significantly (P≤0.05) decreased spore germination of Phaeiosariopsis griseola in vitro. The most effective was Allium sativum extract and Azadirachta indica at 100% extract concentrations. Tithonia diversifolia had moderate fungicidal effect. This study show that all the plants contain most of the active phytochemical compounds which justify their fungicidal property and extract effectiveness increased with their concentration. Therefore, there is possibility that Azadirachta indica, Allium sativum and Tithonia diversifolia extracts can be used to control Phaeiosariopsis griseola of common bean. Further studies are required to quantify these phytochemicals of these plants for the development of cost-effective pesticides for the control of Phaeoisariopsis griseola.

| Views 124 |

| Views 124 |

Phytochemical and fungicidal activity of selected plant extracts against Phaeoisariopsis griseola of common bean

Abdulaziz BK, Musa DD, Aisha H. 2018. Antifungal activity of garlic (Allium sativum) extract on some selected fungi. Journal of Medicinal Herbs and Ethnomedicine 4, 12-14.

Ahmed IM. 2008. Bioactivity of neem (Azadirachta indica) callus extract. Biosciences, Advance Technology and Environment Council 2, 223-238.

Al hazmi RH. 2013. Effect of neem (Azadirachta indica) leaves and seeds extract on the growth of six of the plant diseases causing fungi. Global Advanced Research Journal of Microbiology 2, 89-98.

Allorent D, Savary S. 2005. Epidemiological characteristics of angular leaf spot of bean: A systems analysis. European Journal of Plant Pathology 113, 329–341.

Baba SA, Malik SA. 2015. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Integrative Medicine Research 9, 449-454.

Baljeet SY, Simmy G, Ritika Y, Roshanlal Y. 2015. Antimicrobial activity of individual and combined extracts of selected spices against some pathogenic and food spoilage microorganisms. International Food Research Journal 22, 2594-2600.

Bode S, Tryphone GM, Chilagane LA, Protas D, Kusolwa PM., Nchimbi-msolla S. 2017. Marker assisted selection for common bean diseases   improvements in Tanzania. Prospects and Future Needs 2, 34-45.

Byrappa M. 2015. Antimicrobial activity of Allium sativum against Escherichia coli bacterium. International Journal of Institutional Pharmacy and Life Sciences 5, 215.

Cecilia P, Olubunmi F. 2014. Management of foliar and soil-borne pathogens of cowpea (Vigna unguiculata L. Walp) with two garlic varieties (Allium Sativum L.), African Journal of Biotechnology 13, 1791-1795.

Cherkupally R, Kota SR, Amballa H, Reddy BN. 2017. In vitro antifungal potential of plant extracts against Fusarium oxysporum, Rhizoctonia solani and Macrophomina phaseolina. Annals of Plant Sciences 6, 1676-1680.

Damiano AH, Romero AJ, Simon BA, Huerta ML, Cabrara EH. 2013. The nutritional value of beans (Phaseolus vulgaris L.) and its importance for feeding of rural communities in Puebla-Mexico, International Research Journal of Biological Sciences 2, 59-65.

Ddamulira G, Mukankusi C, Ochwo-Ssemakula M, Sseruwagi P, Edema R, Gepts P. 2014. Distribution and variability of Phaeoisariopsis griseola in Uganda. Journal of Agricultural     Science 6, 16-29.

Effiong EB, Gwana AM, Halima MB, Bagudu BY. 2016. Phytochemical analysis and antimicrobial activity of methanolic, ethanolic and acetonic extracts of stem bark and leaf of neem plant (Azadirachta indica) Journal of Diseases and Medicinal Plant 2, 14-25.

Emitaro WO, Musyimi DM, Otiato DA, Onyango B. 2017. Morphological, biochemical and molecular characterization of Xanthomonas campestris pv. vesicatoria of African nightshades (Solanum scabrum Mill.). International Journal of Biosciences 11, 190-197.

Esmaeili AK, Taha RM, Banisalam B, Mohajer S, Zalina N. 2013. Antimicrobial activities of extracts derived from in vivo and in vitro grown Trifolium pratense (Red clover), International Journal of Environmental Science and Development 4, 475–478.

Ezeonu CS, Chinedu I, Dawn IA, Assumpta I, Abraham J. 2018. Antifungal effect of aqueous and ethanolic extracts of neem leaves, stem bark and seeds on fungal rot diseases of yam and cocoyam. Chemical, Biological Technologies in Agriculture 5, 18.

Hunasagi BS, Somashekhar M, Kalyane NV, Gaviraj EN. 2018. Phytochemical investigation & wound healing activity of Jasminum grandiflorum, Journal of Pharmacognosy & Phytochemistry 7, 31-34.

Katungi E, Sperling L, Karanja D, Farrow A, Beebe S. 2011. Relative importance of common bean attributes and variety demand in the drought areas of Kenya, Journal of Development & Agricultural Economics 3, 411-422.

Keerio A, Nizamani ZA, Hussain S, Rafiq M, Iqbal S, Ahmed S. 2017. To evaluate different botanical extracts against Fusarium oxysporum in-vitro condition, the causal agent of sunflower wilt. European Journal of Biotechnology and Bioscience 2, 25-29.

Keller B, Manzanares C, Jara C, David J, Bruno L. 2015. Fine ‑ mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 4, 813-826.

Leitich RK, Omayio DO, Mukoye B, Mangeni BC, Wosula DW, Arinaitwe W, Abang MM. 2016. Pathogenic variability of angular leaf spot disease of common bean in Western Kenya, International Journal of Applied Agricultural Sciences 2, 92-98.

Mauricio J, Hoyos Á, Alves E, Rozwalka LC, Souza EA, De, Zeviani WM. 2012. Antifungal activity and ultrastructural alterations in Phaeoisariopsis griseola treated with essential oils, Agricultural Sciences 36, 270-284.

Mibei KE, Ojijo KN, Karanja SM, Kinyua KJ. 2012. Phytochemical and antioxidant analysis of methanolic extracts of four African indigenous leafy vegetables. Food Science and Technology, 13, 37-42.

Narasimha MK, Srinivas C. 2012. In vitro screening of bio antagonistic agents and plant extracts to control bacterial wilt (Ralstonia solanacearum) of tomato (Lycopersicon esculentum). Journal of Agricultural Technology 8, 999-1015.

Narayana N, Kola B, Mallapuram N, Yamparala K, Naidu N. 2018. Antioxidant activity of the combined ethanolic extracts of bark of the plants Hemidesmus indicus and Ficus religiosa, World Journal of Pharmacy & Pharmaceutical Sciences 7, 1807-1814.

Obongoya BO, Wagai SO, Odhiambo G. 2010. Phytotoxic effect of selected crude plant extracts on soil-borne fungi of common bean. African Crop Science Journal, 18, 15-22.

Onaran A, Sağlam HD. 2016. Antifungal activity of some plant extracts against different plant pathogenic fungi, International Journal of Advances in Agriculture and Environmental Engineering, 3, 284-287.

Pamela P, Mawejje D, Ugen M. 2014. Severity of angular leaf spot and rust diseases on common beans in Central Uganda, Uganda Journal of Agricultural sciences 15, 63-72.

Pankaj S, Lokeshwar T, Mukesh B, Vishnu B. 2011. Review on neem (Azadirachta indica): Thousand problems one solution, International Research Journal of Pharmacy 2, 97-102.

Parekh J, Chanda S. 2007. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. African Journal of Biomedical Research 31, 53-58.

Parithra CS, Suchiritha D, Jessie WS, Anila BK, Rani VD. 2017. Antioxidant activity of papaya reel & developed chapatis, International Journal of Current Microbiology & Applied Sciences 6, 636-640.

Quy DD, Artik EA, Phuong LT, Lien, HH, Felycia ES, Suradi I, Yi-Hsu J. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, & antioxidant activity of Limophila aromatica, Journal of Food and Drug Analysis 22, 296-302.

Ramadass N, Subramanian N. 2018. Study of phytochemical screening of neem (Azadirachta indica), International Journal of Zoology Studies 3, 209-212.

Rasaga CY, Tepora MM, Rideout JA. 2014. Terpenoids from Tithonia diversifolia, Journal of Research in Science Computing and Engineering 4, 1-7.

Ravishankar TL, Ramneek K, Sukirat K, Shyamalima B. 2018. Neem (Azadirachta indica): An elixir in dentistry. Chronicles of Dental Research, 8, 7-13.

Rejeki D, Addy HS. 2017. Antimicrobial activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana against plant pathogens, Frontiers in Environmental Microbiology 3, 56-61.

Sarawaneeyaruk S, Krajangsang S, Pringsulaka O. 2015. The effects of neem extract and Azadirachtin on soil microorganisms, Journal of Soil Sciences and Plant Nutrition 15, 1071-1083.

Sartorato A. 2002. Identification of Phaeoisariopsis griseola pathotypes from five states in Brazil, Fitopatologia Brasileira 27, 1-4.

Shabana YM, Abdalla ME, Shanin AA, El-Sawy MM, Draz IS, Youssif AW. 2017. Efficacy of plant extracts in controlling wheat leaf rust disease caused by Puccinia triticina. Egyptian Journal of Basic and Applied Sciences 4. 67-73.

Sharma M, Adikshita P. 2017. Integrated approaches for management of angular leaf spot (Phaeoisariopsis griseola) of French bean. International Journal of Science, Environment and Technology 6, 1555-1559.

Singh KP, Pandey V, Singh H, Shukla DN. 2014. Effect of plant leaf extract on fungal diseases of carrot in spore germination. European Journal of Experimental Biology 4, 138-142.

Stavělíková H. 2008. Morphological characteristics of garlic (Allium sativum L.) genetic resources collection- Information, Horticultural Science, 35, 130-135.

Sultana B, Anwar F, Ashraf M. 2009. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts, Molecules 9, 2167-2180.

Simon M. Obila, David M. Musyimi, Phoebe A. Sikuku.
Phytochemical and fungicidal activity of selected plant extracts against Phaeoisariopsis griseola of common bean.
Int. J. Micro. Myco. 11(5), 1-11, May 2020.
https://innspub.net/ijmm/phytochemical-fungicidal-activity-selected-plant-extracts-phaeoisariopsis-griseola-common-bean/
Copyright © 2020
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update