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Antioxidant glutathione dependent system response to in vivo exposure to cadmium and copper in Perna perna of the Gulf of Annaba (Algeria)

By: Wyllia Khati, Ali Banaoui, Michele Roméo, Yousria Gasmi

Key Words: Toxicology, Metals, Environment, Pollution, Mediterranean, Mussel.

J. Bio. Env. Sci. 12(5), 82-89, May 2018.

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In order to use the antioxidant glutathione-dependent system as a biomarker of oxidative stress, the effect of metals on the metabolism of glutathione has been studied at the gills of the African mussel Perna perna living the easternmost Gulf of Annaba (Algeria). The response of the glutathione-S- transferase activity (GST) and the rates of Glutathione (GSH) was evaluated from these bivalves, after in vivo exposure to three concentrations of Cadmium (50, 100 and 200 μg/l) and copper (10, 15 and 25 μg/l) during 7 days. The analyses showed a significant decrease of GSH levels depending on the concentration of cadmium in the medium compared to controls. For the GSH levels, it significantly decreased in exposed bivalves to different concentrations of copper, while the GST activity was strongly inhibited at 25 μg/l of Cu. In fact, Cadmium seems to increase GST activity using glutathione as a substrate which caused a decrease of GSH rates in the exposed mussels, while at the highest dose tested the GST was not required so other enzymes probably metallothioneins (metals detoxification proteins) support the function of antioxidant defense. In the other hand, bioaccumulation of the two metals (Cd) in exposed mussels seems to be not correlated with added concentrations. This situation can be related to the hypothesis that the antioxidant GSH dependent system is most likely involved in this phenomenon. The tested system in Perna perna, reported in this study may be a good biomarker to assess contamination of the marine environment particularly by metals.

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Antioxidant glutathione dependent system response to in vivo exposure to cadmium and copper in Perna perna of the Gulf of Annaba (Algeria)

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Wyllia Khati, Ali Banaoui, Michele Roméo, Yousria Gasmi.
Antioxidant glutathione dependent system response to in vivo exposure to cadmium and copper in Perna perna of the Gulf of Annaba (Algeria).
J. Bio. Env. Sci. 12(5), 82-89, May 2018.
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