Effects of salt stress on leaf protein patterns of rapeseed (Brassica napus L.) genotypes

Paper Details

Research Paper 01/02/2017
Views (259) Download (6)
current_issue_feature_image
publication_file

Effects of salt stress on leaf protein patterns of rapeseed (Brassica napus L.) genotypes

Nima Dolatabadi, Mahmoud Toorchi
J. Bio. Env. Sci.10( 2), 135-140, February 2017.
Certificate: JBES 2017 [Generate Certificate]

Abstract

Environmental stresses caused a protein disorders in plants and plants overcome to stress by altering the expression of genes which necessary for the synthesis of metabolites, structural proteins and enzymes of some metabolic pathways. To study the effect of NaCl salinity on rapeseed’s protein patterns, twelve spring genotypes were evaluated by hydroponic culture at seedling stage. Experiment was performed on three levels of salinity (0, 175, 350 mM) in three replications. In order to study protein patterns by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method, four weeks after salt stress performing and at the end of the seedling stage, leaf samples were collected. For each genotype in each stress level, proteins were extracted in three samples and electrophoresis was performed for them. 30 reproducible bands were identified by SDS-PAGE, in which 18 were polymorphic. Polymorphic bands divided to two groups. The first group had genetic origins and showed the differences among genotypes and the second one called stress-dependent and were affected by salinity. The 20.1 kDa protein bands were induced by salinity in all genotypes while the protein bands with an approximate 56.7 kDa weight were not shown under salt stress. Cluster analysis of data which obtained from protein patterns based on polymorphic bands were used to classification, which had shown high similarity with genotype grouping basis of physiological, biochemical and agronomical data.

VIEWS 7

Ashraf M, McNeilly T. 2004. Salinity Tolerance in Brassica Oilseeds. Critical Reviews in Plant Sciences 23, 157-174.

Ashraf M, Nazir N, McNeilly T. 2001. Comparative salt tolerance of amphidiploid and diploid Brassica species. Plant Science 160, 683-689.

Bandehagh A, Salekdeh GH, Toorchi M, Mohammadi A, Komatsu S. 2011. Comparative proteomic analysis of canola leaves under salinity stress. Proteomics 11, 1965-1975.

Bandehagh A, Uliaie ED, Salekdeh GH. 2013. Proteomic analysis of rapeseed (Brassica napus L.) seedling roots under salt stress. Annals of Biological Research 4, 212-221.

Dolatabadi N. 2008. Evaluation of sodium chloride stress tolerance and protein pattern in rapeseed genotypes (Brassica napus L.). MSc. Thesis. University of Tabriz. Iran. 138p.

Dolatabadi N, Toorchi M, Shakiba M-R, Kazemnia H, Komatsu S. 2012. The response and protein pattern of spring rapeseed genotypes to sodium chloride stress. African Journal of Agricultural Research 7, 755-763.

Gan L, Li D-R, Zang X, Fu C-H, Yu L-J, Li M-T. 2010. Construction of Two-Dimensional Polyacrylamide Gel Electrophoresis System for Proteins in Brassica napus. Acta Agronomica Sinica 36, 612-619.

Joseph B, Jini D. 2010. Proteomic analysis of salinity stress-responsive proteins in plants. Asian Journal of Plant Sciences 9, 307-313.

LAEMMLI UK. 1970. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature 227, 680-685.

Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety 60, 324-349.

Parihar P, Singh S, Singh R, Singh VP, Prasad SM. 2014. Effect of salinity stress on plants and its tolerance strategies: a review. Environmental Science and Pollution Research 22, 4056-4075.

Parvaiz A, Satyawati S. 2008. Salt stress and phyto-biochemical responses of plants – a review. Plant Soil Environment 54, 89-99.

Purty RS, Kumar G, Singla-Pareek SL, Pareek A. 2008. Towards salinity tolerance in Brassica: An overview. Physiology and Molecular Biology of Plants 14, 39-49.

Rossignol M, Peltier JB, Mock HP, Matros A, Maldonado AM, Jorrín J V. 2006. Plant proteome analysis: A 2004-2006 update. Proteomics 6, 5529-5548.

Seki M, Kamei A, Yamaguchi-Shinozaki K, Shinozaki K. 2003. Molecular responses to drought, salinity and frost: Common and different paths for plant protection. Current Opinion in Biotechnology 14, 194-199.

Sha Valli Khan PS, Hoffmann L, Renaut J, Hausman JF. 2007. Current initiatives in proteomics for the analysis of plant salt tolerance. Current Science 93, 807-817.

Shirazi MU, Rajput MT, ANSARI R, Khan MA, TAHIR SS. 2011. Salt tolerance in Brassica species at early seedling stage. Sindh University Research Journal-SURJ (Science Series) 43, 203-208.

Sobhanian H, Aghaei K, Komatsu S. 2011. Changes in the plant proteome resulting from salt stress: Toward the creation of salt-tolerant crops? Journal of Proteomics 74, 1323-1337.

Turan S, Cornish K, Kumar S. 2012. Review article Salinity tolerance in plants : Breeding and genetic engineering. Australian Journal of Crop Science 6, 1337-1348.

Vahdati K, Leslie C. 2013. Abiotic Stress – Plant Responses and Applications in Agriculture (K Vahdati, Ed.). In Tech. 397p.