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Estimation and conformation of HMW glutenin loci in Pakistani Barley Lines detected through polyacrylamide gel electrophoresis

By: Murad Ali, Manzoor Hussain, Mohammad Nisar, Ashutus Singha, Waqar Khan, Shams U Zaman, Arshad Khan

Key Words: Barley, Selected lines, Glutenin protein, SDS-PAGE.

J. Bio. Env. Sci. 14(4), 27-33, April 2019.

Certification: jbes 2019 0139 [Generate Certificate]

Abstract

Glutenin, is consider the major storage proteins in the family of Poaceace which is also consider the main contents in wheat cereal of this family, to commercialize the ancient domesticated cereal like barley in the race of industrial crop and determining the uses of barley flour quality and resemblance of barley elite lines elasticity and dough quality, experimental assay was designed in the Laboratory of Plant Molecular Biology Department of Botany, university of Malakand on 100 elite lines of the barley.to detect High Molecular weight Glutenin protein extracted from the Barley seeds on the basis of Molecular weight, the Glu loci in barley 100 lines which score from the gel of polyacrylamide classify into nine different size (120, 105, 90, 80, 65, 45, 30, 20 and 10 KD) bands which further was classified into five different groups on the basis of variability present in selected barley lines and also in their Molecular weight; absence/presence and intensity of bands were observed. Largest group was observed is group 1 have 41% of elite lines in which 120, 105, 90, 80 and 65 KD molecular weight bands were recorded present. And some of the lines were found unknown which are not related any of these five group respectively, which clearly determine the novel allelic subunit and also show variation from the gene pool in these lines, further evaluation of these lines are needed to track these loci in this cash crop.

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Estimation and conformation of HMW glutenin loci in Pakistani Barley Lines detected through polyacrylamide gel electrophoresis

Ahmad Z, Ajmal SU, Munir M, Zubair M, Masood MS. 2008. Genetic diversity for morpho-genetic traits in barley germplasm. Pak. J. Bot  40(3), 1217-1224.

Anwar A, Masood S, Khan MA, Nasim S. 2003. The high molecular weight glutenin sub unit composition of wheat (Triticum aestivum) landraces from Pakistan. Pak. J. Bot 35(1), 61-68.

Badripour H. 2004. Islamic republic of Iran Country pasture/forage resource profiles. Rangeland management expert in the technical bureau of rangeland.

Chmelík J, Rehulka P, Strelcova M, Kuban V, Mayrhofer C, Allmaier G. 2002. Proteomic analysis of different extracts from barley grains. Rostlinna Vyroba 48(6), 261-264.

Cross RJ, Guo B. 1993. Glutenin variation in a diverse Pre.1935 world wheat germplasm collection, In: Biodiversity and wheat improvement (Ed.): A.B. Damanis. John Wiley and Sons Publishers, U.K pp. 287-298.

Davila JA, Sánchez de la Hoz MP, Loarce Y, Ferrer E. 1998. The use of random amplified microsatellite polymorphic DNA and coefficients of parentage to determine genetic relationships in barley. Genome 41(4), 477-486.

Hou YC, Yan ZH, Wei YM, Zheng YL. 2005. Genetic diversity in barley from West China. Barley Genet. Newsletter 35, 9-22.

Jana S. 1999. Some recent issues on the conservation of crop genetic resources in developing countries. Genome 44, 562-569.

Kirkman MA, Shewry PR, Miflin BJ. 1982. The effect of nitrogen nutrition on the lysine content and protein composition of barley seeds. Journal of the Science of Food and Agriculture 33(2), 115-127.

Lagudah ES, MacRitchie F, Halloran GM. 1987. The influence of high-molecular-weight subunits of glutenin from T. tauschii on flour quality of synthetic hexaploid wheat. J. Cereal Sci 5, 129-138.

Manjunatha T, Bisht IS, Bhat KV, Singh BP. 2006. Genetic diversity in barley (Hordeum vulgare L.) landraces from Uttaranchal. Genet. Resources and Crop Evolution 54(1), 55-65.

Payne PI, Corfield KG, Blackman JA. 1979. Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheat of related pedigree. Theoretical and Applied Genetics 55(3-4), 153-159.

Payne PI, Corfield KG, Holt LM, Blackman JA. 1981. Correlations between the inheritance of certain high‐molecular weight subunits of glutenin and bread‐making quality in progenies of six crosses of bread wheat. Journal of the Science of Food and Agriculture 32(1), 51-60.

Payne PI, Holt LM, Jackson EA, Law CN. 1984. Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Phil. Trans. R. Soc. Lond. B 304(1120), 359-371.

Payne PI, Holt LM, Law CN. 1981. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Theoretical and Applied Genetics 60(4), 229-236.

Russel JR, Fuller JD, Macaulay M, Hatz BG, Jahoor A, Powell W, Waugh R. 1997. Direct comparison of levels of genetic variation among barley accessions detected by RFLP, AFLPs, SSRs and RAPDs. Theor. Appl. Genet 95, 714-722.

Sorrels ME, Wilson WA. 1997. Direct classification and selection of superior alleles for crop improvement. Crop Sci 37, 691-697.

Tahir M, Turchelta T, Anarw R, Lafiandra D. 1996. Assessment of genetic variability in Vallega, V and T. Mello-Sampayo. 1987. Variation of high molecular- weight glutenin subunits amongst cultivars of Triticum turgidum L. from Portugal. Euphytica 3, 755-762.

Murad Ali, Manzoor Hussain, Mohammad Nisar, Ashutus Singha, Waqar Khan, Shams U Zaman, Arshad Khan.
Estimation and conformation of HMW glutenin loci in Pakistani Barley Lines detected through polyacrylamide gel electrophoresis.
J. Bio. Env. Sci. 14(4), 27-33, April 2019.
https://innspub.net/jbes/estimation-conformation-hmw-glutenin-loci-pakistani-barley-lines-detected-polyacrylamide-gel-electrophoresis/
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