International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Estimation of molecular diversity of Berberis lycium in three districts of Azad Jammu and Kashmir

By: Rizwan Azim, Muhammad Ilyas, Shahid Iqbal Awan, Adnan Rasheed, Mustansar Shakil

Key Words: Berberis lycium, cluster analysis, genetic variation, Molecular diversity.

J. Bio. Env. Sci. 12(5), 32-39, May 2018.

Certification: jbes 2018 0028 [Generate Certificate]

Abstract

Research study was carried in the Pant Genetic Resources Institute (PGRI), NARC Islamabad for the assessment of molecular diversity of Berberis lyciumin three districts of Azad Jammu and Kashmir. The present research describes the importance genetic diversity of medicinally important Berberis specie Berberberis lycium from great mountainous regions of AJK. Nine locations i.e; Hurnamaira, Datot, Topa, Tolipeer, Hullar, Morifurman Shah, Trarakhal, Baloch and Pullandri were selected on the basis of different altitudes and agroclimatic conditions and mature fruits were harvested from different barberry ecotypes. The matured harvested fruits were kept in the plastic pots and transported to Pant Genetic Resources Institute (PGRI), NARC Islamabad where they were subjected to -80 ˚C. Seeds were separated from the fruit pulp and used for protein analysis. SDS PAGE technique was used for the molecular characterization of selected ecotypes. Ecotypes Morifurman Shah and Tolipeer showed maximum genetic variation on the basis of molecular studies.

| Views 84 |

Estimation of molecular diversity of Berberis lycium in three districts of Azad Jammu and Kashmir

Abd-El-Zaher M, Badr MA, El-Galaly A, Ahmed MA, Mobarak A, Hussan GM. 2006. Genetic diversity among Ocimum populations in Egypt as reflected by morphological, seed protein and isozyme polymorphism. International journal of Botany 2, 261-269.

Abd E, Elham A, Atef HA, Nagwa A. 2010.Phylogenetic diversity and relationships of some tomato varieties by electrophoretic protein and RAPD analysis. Journal of American Sciences 6, 434-441.

Ahrendt LWA. 1941. A survey of the genus Berberis. In Asia. Journal of Botany 79,1-64.

Ahrendt LWA. 1961. Berberis and Mahonia: a taxonomic revision. Journal of Linnaean Society of London, Botany 57, 1-410.

Asif A, Kakub G, Mehmood S, Khunum R, Gulfraz M. 2007. Wound healing activity of root extracts of Berberis lycium Royle in rats. Journal of Phytotherapy Research 21, 589-591.

Bhattacharjee SK. 1990. Handbook of Medicinal Plants, Pointer Publishers, Fourth Edition.

Bruckner C. 2000. Clarification of the Carpel Numberin Papaverales, Capparales, and Berberidaceae. Botanical Review 66, 155-307.

Chiang H, Hanssen CB. 1977. The Alkalods. Journal of Organic Chemistry 42, 3190-3192.

Chauhan NS. 1990. Medicinal and aromatic plants of Himachal Pradesh, National work-shop on Cold Desert Area, India. Govt Press, H. P Shimla, 65-68 p.

Hassan AK, Ahmad M. 2007. Autheticity of folk medicinal plants of Pakistan. Taxonomic and Chemical Methods 2, 37-38.

Huxley A. 1992. The New RHS Dictionary of Gardening, MacMillan Press.

Imanshahidi M, Hosseinzade H.2008. Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Journal of Phytotherapy Research 22, 999-1012.

Khan A. 2001. Ethnobotanical potential, Phytosociology and conservation status of Mount Elum, Buner. M.Phil Thesis, Pakistan.

Kulkarni SK, Dhir A. 2010. Berberine: a plant alkaloid with therapeutic potential for central nervous system disorders. Journal of Phytotherapy Research 24, 317-324.

Khare CP. 2004. Indian Herbal Remedies, Springer, New York, 98-100.

Khan M, Giessrigl B, Vonachb C, Madlenerb S, Prinzc S, Herbaceckd I, Hölzld C, Bauerb S, Violab K, Mikulitsd W, Quereshia RA, Knasmüllerd S, Gruschd M, Koppc B, Krupitzab G. 2010. Berberine and a Berberis lycium extract inactivate Cdc25A and induce a-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis. Journal of Phototherapy Research 683, 123-130.

Lewis K, Ausubel FM. 2006. Prospects for plant derived antibacterial. Journal of Nature Biotechnology 24, 1504-1507.

Landrum LR. 1999. Revision of Berberis (Berberidaceae) in Chile and adjacent southern Argentina. Annals of the Missouri Botanical Garden, 793-834.

Rao RR, Husain T, Dutt B, Garg A. 1998. Revision of the family Berberidaceae of India I. Rheedea, 8, 1-66.

Rao R, Hajra PK. 1998. Berberis; Flora of India. Volume 1. Calcutta: Botanical Survey of India. 351-413 p.

Rao RR, Hajra KP. 1993. Berberis. Flora of India 1, 325-404p.

Rohlf FJ. 2005. NTSYS-pc: Numerical taxonomy system. Exeter Publishing Ltd., Setauket. New York.

Shah GM, Khan MA. 2006. Common Medicinal Folk Recipes of Siran Valley, Mansehra-Pakistan. Pakistan Journal Pharmacological Sciences 10, 49-62.

Safeer A, Faria S. 2012. Voltammetric determination of Antioxidant Character in Berberis lycium, Zanthoxylum armatum and Morusnigra Lin. Pakistan Journal    Pharmacological Sciences 25, 501-507.

Said HM. 1996.Medicinal Herbs-A Textbook for Medical Students and Doctors, 1,Hamdard Foundation, Nazimabad, Karachi, Sindh, Pakistan.

Sharma R. 2003. Medicinal Plants of India An Encylopedia, Daya Publishing House, Delhi, 33-34 p.

Tiwari UL, Adhikari BS, Rawat GS. 2012. A checklist of Berberidaceae in Uttarakhand, Western Himalaya, India. Check List 8, 610-616.

Yip PY, Chau CF, Mak CY, Kwan HS. 2007. DNA methods for identification of Chinese medicinal materials. Journal of Chinese Medicine 10, 1186-1749.

Rizwan Azim, Muhammad Ilyas, Shahid Iqbal Awan, Adnan Rasheed, Mustansar Shakil.
Estimation of molecular diversity of Berberis lycium in three districts of Azad Jammu and Kashmir.
J. Bio. Env. Sci. 12(5), 32-39, May 2018.
https://innspub.net/jbes/estimation-molecular-diversity-berberis-lycium-three-districts-azad-jammu-kashmir/
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update