Genetic Diversity in Egyptian Populations of Tilapia species Using RAPD and SRAP Markers
By: Gomaah El Fadly, Ismael Khatab, Medhat Rehan, Alaa Kalboush
Key Words: Genetic diversity, Population differentiation, RAPD, SRAP, Tilapia species.
J. Bio. Env. Sci. 8(4), 231-243, April 2016.[Generate Certificate]
This study aimed to estimate the genetic diversity among and within populations of three Tilapia species e.g., Oreochromis niloticus, Tilapia zilli and Oreochromis aurea which were collected from different locations i.e., Ryad, Motobs (Kafr El Sheikh Governorate), and Bahr El Baqar (Sharqia Governorate).Random amplified polymorphic DNA (RAPD) and sequence related amplified polymorphism (SRAP) were used in this study. Our results indicated that percentages of polymorphism were 58.41, 41.87 and 31.49% according to RAPD whereas 68.96, 33.33 and 17.64% terms of SRAP for O. niloticus, T. zilli and O. aurea respectively. The percentages of polymorphism using RAPD and SRAP within O. niloticus populations were the highest values . Population from Ryad was closer to population of Bahr El Baqar in O. niloticus, while population of T. zilli and O. aurea from Bahr El Baqar was more similar to population from Motobs. The outcome of this study considered as baseline of genetic diversity which will be useful in breeding program and conservation biology of Tilapia species and a baseline analysis of the population differentiation found among of Tilapia species in Egypt.