Isolation and Identification of Keratin Degrading (keratinolytic) Bacteria from Poultry Feather Dumping Sites
By: Sunil Chhimpa, Chandra Shekhar Yadav, P.J. John
Key Words: Poultry feather, Biodegradation, Keratinase, Bacillus subtilis.
J. Bio. Env. Sci. 8(6), 109-119, June 2016.[Generate Certificate]
Poultry industries generate a large quantity of feather waste. These feathers contain high quantity of keratin which can be bio-converted into peptides and amino acids using bacteria to make value added products. Therefore, the aim of this study was to isolate and identify keratinolytic bacteria from poultry feather dumping sites at Jaipur city, Rajasthan, India. The samples such as soil and feathers were collected and screened for keratinolytic bacteria. The samples were inoculated on the feather meal agar plates and incubated at 35o C for 24-48 hrs. The culture obtained from the feather meal agar plate was sub cultured on feather meal broth. The bacterium which showed maximum kertainolytic activity was subjected to morphological and biochemical tests. Molecular characterization of the isolated bacteria was done by 16S rRNA sequencing and identified as Bacillus subtilis strain AJ. Culture conditions such as pH, incubation temperature, agitation and duration were optimized for maximum feather degradation. The bacteria B. subtilis showed maximum feather degradation at pH 7 at 300 C with an agitation of 140 rpm for 96 hrs. Isolation, purification and characterization of keratinase need to be done to assess the industrial application of the isolate.