International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Mitochondrial gene marker sequences reveal identities of gobies at Minalungao National Park, Nueva Ecija, Philippines

By: Khristina G. Judan Cruz, Melinda N. Reyes, Perry Ayn Mayson A. Maza, Revin Arsee P. Cardona, Wilson R. Jacinto

Key Words: Gobiidae, Mitochondrial sequences, Gobiidae, Minalungao National Park

J. Bio. Env. Sci. 13(2), 258-264, August 2018.

Certification: jbes 2018 0021 [Generate Certificate]

Abstract

Gobiidae is a rapidly growing family besides being the largest, with new species continuously discovered in all parts of the world, specifically in Asia and in the Philippines.  Given this diversity, this study provides an initial identification and is the first report of gobies found at Minalungao National Park, a Key Biodiversity Area (KBA) in Central Luzon, Philippines. Goby species were identified molecularly using mitochondrial genes namely:  CO1 (Cytochrome Oxidase 1), Control Region or D-Loop, and 16s rRNA. Samples were collected along Minalungao River. DNA was extracted from muscle tissues and gene markers were amplified using polymerase chain reaction. Sequences were subjected to BLAST for species identification. Results showed CO1 sequences ranging from 712-719 bp; D-Loop at 781-841 bp; and 559-592 bp in 16srRNA. BLAST sequences revealed four species of gobies belonging to 2 genera namely Rhinogobius giurinus (99% maximum identity using 16srRNA, 95% with D-Loop), Rhinogobius brunneus (97% matched using CO1), Glossogobius bicirrhosus (82% matched with D-Loop), and Glossogobius giuris (with 87% CO1 match).

| Views 23 |

Mitochondrial gene marker sequences reveal identities of gobies at Minalungao National Park, Nueva Ecija, Philippines

Akihito Y, Meguro K. 1975. First record of Glossobius celebius from Japan. Japanese Journal of Icthyology21(4), 227-230.

Allen GR. 1991. Field guide to the freshwater fishes of New Guinea. Publication, no. 9. 268 p. Christensen Research Institute, Madang, Papua New Guinea.

Angers B, Bernatchez L. 1998. Combined Use of SMM and Non-SMM Methods to Infer Fine Structure and Evolutionary History of Closely Related Brook Charr (Salvelinus fontinalis, Salmonidae) Populations from Microsatellites.Molecular Biology and Evolution15(2),143–159. 1998

Bagorodsky S,  Kovacic M, Ozen O, Bilecenogin M. 2010. Records of two uncommon goby species (Millerigobius macrocephalus, Zebrus zebrus) from the Aegean Sea. Acta Adriatica 51(2),217- 222.

Bucol AA, Carumbana EE. 2010. Checklist of fishes found in the fresh and brackish waters of Negros and Siquijor, Philippines. Asian Scientific Journals 1,1.

Carpenter KE, Springer VG. 2005. Environmental Biology of Fishes 72, 467-480.

Chauhan T,Raijiv K. 2010. Molecular markers and their applications in fisheries and aquaculture. Advances in Bioscience and Biotechnology, 2010 1, 281-291.

Chen IS, Han CC, Fang LS. 1995. A new record of freshwater gobiid fish, Schismatogobius roxasi (Pisces, Gobiidae) from South-eastern Taiwan. Bulletin of the National Museum of Nature and Science6,135-137.

Chen IS, Shao KT. 1996. A taxonomic review of the gobiid fish genus Rhinogobius Gill, 1859, from Taiwan, with descriptions of three new species. Zoological Studies 35(3),200-214.

Chen IS, Hsu CH, Hui CF, Shao KT,  Miller PJFang LS. 1997. Sequence length and variation in the mitochondrial control region of two freshwater gobiid fishes belonging to Rhinogobius (Teleostei: Gobioidei).Journal of Fish Biology 53, 179–191.

Chen IS, Wu HL, Shao KT. 1999. A new species of Rhinogobius (Teleostei: Gobiidae) from Fujian Province, China. Ichthyological Research 46(2),171 -178.

Chen I, Kottelat M, Miller PJ. 1999. Freshwater gobies of the genus Rhinogobius from the Mekong basin in Thailand and Laos, with descriptions of three new species. Zoological Studies 38, 19-32.

Chen IS, Kottelat M. 2003. Three new freshwater gobies of the genus Rhinogobius (Teleostei: Gobiidae) from North-eastern Laos. The Raffles Bulletin of Zoology 51(1),87-95.

Chen IS, Tan HH. 2005. A new species of freshwater goby (Teleostei: Gobiidae: Stiphodon) from Pulau Tioman, Pahang, Peninsular Malaysia. The Raffles Bulletin of Zoology 53(2), 237-242.

Chen IS, Fang LS. 2006. A new species of Rhinogobius (Teleostei: Gobiidae) from the Hanjiang Basin in Guangdong Province, China. Ichthyological Reseach53(3), 247 – 253.

Chen IS, MillerPJ. 2008. Two new freshwater gobies of genus Rhinogobius (Teleostei: Gobiidae) in southern China around the northern region of the South China Sea. The Raffles Bulletin of Zoology 19, 225-232.

Conservation International Philippines, Department of Environment and Natural Resources-Protected Areas and Wildlife Bureau, Haribon Foundation for the Conservation of Nature. 2006. Priority Sites for Conservation in the Philippines: Key Biodiversity Areas. Quezon City, Philippines: Conservation International Philippines. Published online at

http://www.conservation.org/global/philippines.

Conservation International.2013. Biological diversity in the Philippines. Retrieved from http://www.eoearth.org/view/article/1506.48

Department of Environment and Natural Resources – Protected Areas and Wildlife Bureau and Haribon Foundation. 2006. Retrievedat

www.bmb.gov.ph.

Devi R, Boguskaya N. 2009.Rhinogobius brunneus. The IUCN Red List of Threatened Species 2009: e.T166994A6296768. Downloaded on 05 April 2018

http://dx.doi.org/10.2305/IUCN.UK.20092.RLTS.T166994A6296768.en

Froese R, Pauly D (Eds.).  2006.  FishBase.  World Wide Web electronic publication.  www.fishbase.org,version03/2006

Greenfield DW, Jewett SL. 2011. Eviota rubriceps, a new goby from the South-western Pacific Ocean, with comments on E. mikiae and E. raja (Teleostei: Gobiidae). Zootaxa 3134,53–62.

Herre AW. 1927. Gobies of the Philippines and the China Sea. Monographs of the Bureau of Science 23. Philippine Bureau of Science, Manila.

Hossain MY, Ohtomi J, Ahmed ZF, Ibrahim AHM, Jasmine S. 2009. Length-weight and morphometric relationships of the tank goby Glossogobius giuris (Hamilton, 1822) (Perciformes: Gobiidae) in the Ganges of Northwestern Bangladesh. Asian Fisheries Science 22(3), 961-969.

Huang SP, Chen LS. 2007. Three new species of Rhinogobius Gill, 1859 (Teleostei: Gobiidae) from the Hanjiang basin, Southern China. The Raffles Bulletin of Zoology 14,101-110.

Huckstorf V. 2012. Rhinogobius giurinus. The IUCN Red List of Threatened Species. Version 2014.2. www.iucnredlist.org

Jaafar Z, Larson HK. 2008. A new species of mudskipper, Periophthalmus takita (Teleostei: Gobiidae: Oxudercinae), from Australia, with a key to the genus. Zoological Science 25(9),946 – 952.

Kannan KK, Sureshkumar L, Ranjith KK, Joshi, Madan MS, John S. 2013. First record of the two-stripe goby, Valenciennea helsdingenii (Gobiidae, Gobiiformes) from the southeast coast of India. ZooKeys 323,91–97.

Keith P,  Vigneux E, Bosc P. 1999. Atlas des poissons et des crustacés d’eau douce de La Réunion. Muséum national d’Histoire naturelle, Paris. Patrimoines Naturels 39, 136.

Keith P,  Lord C, Lorion J,  Watanabe S, Tsukamoto K, Couloux A, Dettai A. 2010. Phylogeny and biogeography of Sicydiinae (Teleostei: Gobiidae) inferred from mitochondrial and nuclear genes. Marine Biology 158 (2),311 – 326.

Kok HM, Blaber SJM. 1976. A new freshwater goby (Teleostei: Gobiidae) from the Pongolo floodplain, Zululand, South Africa. Zoologica Africana 12(1), 163-168.

Kottelat M, Whitten AJ,Kartikasari SN, Wirjoatmodjo S. 1993. Freshwater fishes of Western Indonesia and Sulawesi. Periplus Editions, Hong Kong. 221 p.

Larson H. 2012. Glossogobius bicirrhosus. The IUCN Red List of Threatened Species. Version 2014.2. www.iucnredlist.org

Liu ZJ, Cordes JF. 2004.  DNA marker technologies and their applications in aquaculture genetics; a review. Aquaculture 238,1-37.

www.elsevier.com/locate/aquaonline

Masuda H, Amaoka K, Araga C, Uyeno T,  Yoshino T. 1984. The fishes of the Japanese Archipelago. Tokai University Press, Tokyo, Japan.

Miller PJ, Wongrat P. 1979. A new goby (Teleostei: Gobiidae) from the South China Sea and its significance for gobioid classification. Zoological Journal of the Linnean Society 67(3),239 – 257.

Myers P, Espinosa C, Parr CS, Jones T, Hammond GS, Dewey A. 2012. The Animal Diversity Web (online). Available at

http://animaldiversity.org

Nguyen VH. 2005. Freshwater fishes of Vietnam. Agricultural Publishing House 3.

Rasmussen RS, Morrissey M. 2008. DNA-Based Methods for the Identification of Commercial Fish and Seafood Species. Comprehensive Reviews in Food Science and Food Safety. Volume 7, Issue 3 June 2008 Pages 280–295.

Parenti LR, Maciolek JA. 1993. New Sicydiinae gobies from Ponape and Palau, Micronesia, with comments on systematics of the subfamily Sicydiinae (Teleostei: Gobiidae). Bulletin of Marine Science 53(3), 945–972. University of Miami – Rosenstiel School of Marine and Atmospheric Science.

Samonte IE. 2003.  Phylogenetic relationships of endemic Lake Victoria cichlids inferred from mitochondrial and nuclear DNA Data.  An unpublished dissertation.  .Eberhard Karls Universitat Tuebingen.

Sanda R, Kovacic M. 2009. Freshwater gobies in the Adriatic drainage basin of the Western Balkans. Annales – Series Historia Naturalis  19, 1-10.

Sato A, Takezaki N, Tichy H, Figueroa F, Mayer WE, Klein J. 2003. Origin and Speciation of Haplochromine Fishes in East African Crater Lakes Investigated by the Analysis of Their mtDNA, Mhc Genes, and SINEs. Molecular Biology and Evolution 20(9),1448–1462. 2003.

Serov DV, Nezdoliy  VK, Pavlov DS. 2006. The Freshwater Fishes of Central Vietnam. KMK Scientific Press Ltd., Moscow, Nha Trang.

Shibukawa K, Aonuma Y. 2007. Three new species of the deep-dwelling goby genus Obliquogobius (Perciformes: Gobiidae: Gobiinae) from Japan, with comments on the limits of the genus. Bulletin of the National Museum of Nature and Science, p 137–152.

Suk HY, Choe JC.2002a. Females prefer males with larger first dorsal fins in the common freshwater goby. Journal of Fish Biology 61,899-914.

Talwar PK, Jhingran AG. 1991. Inland fishes of India and adjacent countries. Volume 2, A.A. Balkema, Rotterdam.

UPLB Limnological Research Station. 2011. Freshwater fishes in Southern Luzon. University of the Philippines Los Baños, 4031 College, Laguna, Philippines p 1-35.

Vedra SA, Ocampo PP.2013. Morphology of Rhyacichthys aspro (Valenciennes 1837) in Mandulog river system, Iligan city, Northern Mindanao, Philipines. International Journal of Research in BioSciences 2(2),93-105.

Vedra SA, Ocampo PP, De Lara AV, Rebancos CM, Pacardo EP, Briones NM. 2013. Indigenous goby population in Mandulog River System and its conservation by communities in Iligan City, Philippines. Journal of Environmental Science and Management 16(2), 11-18.

Walberg MW, Clayton DA.1981.Nucleic Acids Research9(1981), 5411-5421.

Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding of Australia’s fish species. Philosophical Transactions of the Royal Society B 360, 1847–1857.

Xie S, Cui Y, Li Z. 2001. Small fish communities in two regions of the Liangzi Lake, China, with or without submersed macrophytes. Journal of Applied Ichthyology 17,89-92.

Xie ZY, Du JZ, Chen XQ, Wang YX, Murray BW. 2006. The significance of mitochondria control region (D-Loop) in intraspecific genetic differentiation of fish. Hereditas (Beijing)28,362-368.

Khristina G. Judan Cruz, Melinda N. Reyes, Perry Ayn Mayson A. Maza, Revin Arsee P. Cardona, Wilson R. Jacinto.
Mitochondrial gene marker sequences reveal identities of gobies at Minalungao National Park, Nueva Ecija, Philippines.
J. Bio. Env. Sci. 13(2), 258-264, August 2018.
https://innspub.net/jbes/mitochondrial-gene-marker-sequences-reveal-identities-gobies-minalungao-national-park-nueva-ecija-philippines/
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update