International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Role of biotechnology in protection of endangered medicinal plant, Bunium persicum; the most important herb of Iran

By: Yousef Emamipoor

Key Words: Bunium persicum, Somatic embryogenesis, Plant growth regulators, Corm derived callus

J. Bio. Env. Sci. 12(5), 312-324, May 2018.

Certification: jbes 2018 0051 [Generate Certificate]

Abstract

Bunium persicum (Boiss) Fedtsch is a valuable medicinal plant that is facing extinction. A study was conducted to adopt various strategies and techniques to conserve and protect the biodiversity of B. persicum. Application of corm explant from six-month-old seedling on MS medium supplemented with various auxins showed that corm derived callus on MS medium supplemented with 1.0mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D) by 74.9% induced somatic embryogenesis callus. The somatic embryos transferred to medium supplemented with different concentrations of benzylaminopurine (BAP), kinetin, spermidine, forchlorfenuron (CPPU), chlormequat chloride (CCC), paclobutrazol (PBZ), casein hydrolysate (CH), poly ethylene glycol (PEG) and banana powder, led to maximum plantlet regeneration, which was 65.8 ± 2.6 obtained in ½ MS medium supplemented with 20g/l banana powder. Consequently, induction of somatic embryogenesis under 1.0mg/L 2, 4-D was found to be more suitable than other auxins and capacity of banana powder for plantlet development by having indol acetic acid (IAA), cytokinins and gibberellins (GAs) was more than other additives and PGRs. Also, the effect of different concentrations of sucrose, BAP, PBZ and GA3 on size of B. persicum corms was investigated. Results showed that 90 g/L sucrose with 164.9 ± 2.8 mg corm fresh weight (FW) was the most suitable sucrose concentration for growth of corm and shoot numbers. The corms coated with sodium alginate and calcium nitrate to create artificial seeds. The seeds transferred to soil and produced seeds in the first year.

| Views 71 |

Role of biotechnology in protection of endangered medicinal plant, Bunium persicum; the most important herb of Iran

Abraham J, Cheruvathur MK, Mani B, Thomas TD. 2010. A rapid in vitro multiplication system for commercial propagation of pharmaceutically important Cyclea peltata (Lam) Hook & Thoms. based on enhanced axillary branching. Industrial Crops and Products 31, 92-98.

Ascough GD, Erwin JE, Staden JV. 2008. Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae. Plant Cell, Tissue and Organ Culture 95, 275-283.

Barciszewski J, Massino F, Clark BFC. 2007. Kinetin a multiactive molecule. International Journal of Biological Macromolecules 40, 182-192.

Baskin JM, Baskin CC. 2004. A classification system for seed dormancy. Seed Science Research 14(1-16).

Carter J, Gunawardena AHL. 2011. Regeneration of the aquatic monocot Aponogeton madagascariensis (lace plant) through callus induction. Aquatic Botany 94, 143-149.

Deo PC, Harding RM, Taylor M, Tyagi AP, Becker DK. 2009. Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta). Plant Cell, Tissue and Organ Culture 99, 61-71.

Deo PC, Taylor M, Harding RM, Tyagi AP, Becker DK. 2010. Initiation of embryogenic cell suspensions of taro (Colocasia esculenta var. esculenta) and plant regeneration. Plant Cell, Tissue and Organ Culture 100, 283-291.

El-Hilaly J, Hmammouchi M, Lyoussi B. 2003. Ethnobotanical studies and economic evaluation of medicinal plants in Taounate province. Journal of Ethnopharmacology 86, 149-158.

Emamipoor Y, Maziah M. 2014. An efficient method in breaking of dormancy from Bunium persicum (Boiss) Fedtsch seeds: a valuable herb of Middle East and Central Asia. Asian Pac J Trop Biomed 4(8), 642- 649.

Faravani M. 2004. Survey on treatment of seed amount, transplanting time of black zeera bulbs from nursery to field with best cultivation pattern. Iranian Journal of Pharmaceutical Research 3(2), 65-65.

Ghoncheie M, Emamipoor Y. 2007. Collection, identification, evaluation and protection of medicinal plant’s seeds for gene bank Final reports of research studies. Tehran, Iran: Research Institute of Forests and Rangelands.

Hussain I, Chaudhry Z, Muhammad A, Asghar R, Naqvi SMS, Rashid H. 2006. Effect of chlorocholine chloride, sucrose and BAP on in vitro tuberization in potato (Solanum tuberosum L. cv. cardinal). Pakistan Journal of Botany 38(2), 275-282.

Khan MY, Aliabbas S, Kumar V, Rajkumar S. 2009. Recent advances in medicinal plant biotechnology. Indian Journal of Biotechnology 8(9-22).

Khosravi M. 2005. Intercropping Black Zira (Bunium persicum) with Saffron and annual crops: Agroecological and economic perspetives. Doctor Of Phylosophy PhD thesis, University of Ferdosi, Mashhad, Iran.

Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N. 2010. Chilli peppers – A review on tissue culture and transgenesis. Biotechnology Advances 28, 35-48.

Medina RD, Flachsland EA, Gonzalez AM, Terada G, Faloci MM, Mroginski LA. 2009. In vitro tuberization and plant regeneration from multinodal segment culture of Habenaria bractescens Lindl., an Argentinean wetland orchid. Plant Cell, Tissue and Organ Culture 97, 91-101.

Moon HK, Stomp M. 1997. Effects of medium components and light on callus induction, growth, and frond regeneration in Lemna gibba (Duckweed). In Vitro Cellular and Developmental Biology–Plant 33, 20-25.

Pezhmanmehr M, Hassani ME, Jahansooz F, Najafi AA, Sefidkon F, Mardi M, Pirseiedi M. 2009. Assessment of genetic diversity in some Iranian populations of Bunium persicum using RAPD and AFLP markers. Iranian Journal of Biotechnology 7(2), 93-100.

Prammanee S, Thumjamras S, Chiemsombat P, Pipattanawong N. 2011. Efficient shoot regeneration from direct apical meristem tissue to produce virus-free purple passion fruit plants. Crop Protection 30, 1425-1429.

Russell-Smith J, Karunaratne NS, Mahindapala R. 2006. Rapid inventory of wild medicinal plant populations in Sri Lanka. Biological Conservation 132, 22-32.

Sharma RK, Sharma S, Sharma SS. 2006. Seed germination behaviour of some medicinal plants of Lahaul and Spiti cold desert (Himachal Pradesh): implications for conservation and cultivation. [Research Communications]. Current Science 90(8), 1113-1118.

Silveira V, Floh EIS, Handro W, Guerra MP. 2004. Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda. Plant Cell, Tissue and Organ Culture 76, 53-60.

Singh J, Tiwari KN. 2010. High-frequency in vitro multiplication system for commercial propagation of pharmaceutically important Clitoria ternatea L.—A valuable medicinal plant. Industrial Crops and Products 32, 534-538.

Thatoi H, Patra JK. 2011. Biotechnology and Pharmacological Evaluation of Medicinal Plants: An Overview, Journal of Herbs, Spices & Medicinal Plants 17(3), 214-248.

Wakhlu AK, Nagari S, Barna KS. 1990. Somatic embryogenesis and plant regeneration from callus cultures of Bunium persicum Boiss. Plant Cell Reports 9, 137-138.

Zhang S, Liu N, Sheng A, Ma G, Wu G. 2011. Direct and callus-mediated regeneration of Curcuma soloensis Valeton (Zingiberaceae) and ex vitro performance of regenerated plants Scientia Horticulturae 130, 899-905.

Yousef Emamipoor.
Role of biotechnology in protection of endangered medicinal plant, Bunium persicum; the most important herb of Iran.
J. Bio. Env. Sci. 12(5), 312-324, May 2018.
https://innspub.net/jbes/role-biotechnology-protection-endangered-medicinal-plant-bunium-persicum-important-herb-iran/
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update