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Salt tolerant strains of Sinorhizobium meliloti improved the forage yield and nutrient contents of Medicago sativa under salt toxicity of soil

By: Alireza Tavasolee, Nader Mirfakhraei, Kazem Khavazi, Hossein Besharati, Hassan Monirifar

Key Words: Salinity, Alfalfa, Nutrients, Sinorhizobium meliloti, Forage yield.

J. Bio. Env. Sci. 14(2), 123-130, February 2019.

Certification: jbes 2019 0130 [Generate Certificate]


Salt stress extremely disrupts the growth and developments of plants, whereas nutrients play a vital role in regulating plant responses to salinity stress. Bacteria is attracting increasing attention in recent years as a potential soil amendment under stress condition. This study addressed the use of salt tolerant strains (three strain) of Sinorhizobium meliloti to mitigate salt-stressed soil (7.34 dS m-1) and evaluated the levels of some nutrients in alfalfa (Medicago sativa L.) ecotypes (Kara yonje, Hamedani and Malek kandi). All of the Sinorhizobium meliloti strains under the salt stress, improved the forage yield in ecotype of Hamedani. In ecotype of Malek kandi, inoculation of seeds by strains 1 and 3 increased the dry weight of forage in compared to the non-inoculated crops, strain 2 did not show a tangible effect on forage yield. The most beneficial effect of Sinorhizobium meliloti on rising fresh and dry weights of alfalfa ecotypes occurred in ecotype of Hamedani with strain 2. Inoculation of alfalfa ecotypes by Sinorhizobium meliloti exposed various results depends to ecotypes, but in general in all of the ecotypes, Sinorhizobium meliloti improved the manganese content in plant tissues. This nutrient showed a significant correlation with alfalfa growth under salt stress.

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Salt tolerant strains of Sinorhizobium meliloti improved the forage yield and nutrient contents of Medicago sativa under salt toxicity of soil

Bao AK, Du BQ, Touil L, Kang P, Wang QL, Wang SM. 2016. Co‐expression of tonoplast Cation/H+ antiporter and H+‐pyrophosphatase from xerophyte Zygophyllum xanthoxylum improves alfalfa plant growth under salinity, drought and field conditions. Plant biotechnology journal 14, 964-975.

Benidire L, Lahrouni M, El Khalloufi F, Göttfert M, Oufdou K. 2017. Effects of Rhizobium leguminosarum Inoculation on Growth, Nitrogen Uptake and Mineral Assimilation in Vicia faba Plants under Salinity Stress. Journal of Agricultural Science and Technology 19, 889-901.

Carrasco-Gil S, Rios JJ, Álvarez-Fernández A, Abadía A, García-Mina JM, Abadía J. 2016. Effects of individual and combined metal foliar fertilisers on iron-and manganese-deficient Solanum lycopersicum plants. Plant and soil 402, 27-45.

Egamberdieva D, Lugtenberg B. 2014. Use of plant growth-promoting rhizobacteria to alleviate salinity stress in plants. In Use of Microbes for the Alleviation of Soil Stresses, Volume 1. Springer New York p. 73-96.

Fageria NK. 2016. The use of nutrients in crop plants. CRC press.

Farhangi-Abriz S, Ghassemi-Golezani K. 2018. How can salicylic acid and jasmonic acid mitigate salt toxicity in soybean plants? Ecotoxicology and Environmental Safety 147, 1010-1016.

Farhangi-Abriz S, Torabian S. 2017. Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress. Ecotoxicology and environmental safety 137, 64-70.

Farhangi-Abriz S, Torabian S. 2018 a. Biochar improved nodulation and nitrogen metabolism of soybean under salt stress. Symbiosis 74, 215-223.

Farhangi-Abriz S, Torabian S. 2018b. Effect of biochar on growth and ion contents of bean plant under saline condition. Environmental Science and Pollution Research 25, 11556-11564.

Ghassemi-Golezani K, Farhangi-Abriz S. 2018. Foliar sprays of salicylic acid and jasmonic acid stimulate H+-ATPase activity of tonoplast, nutrient uptake and salt tolerance of soybean. Ecotoxicology and environmental safety 166, 18-25.

Gupta B, Huang B. 2014. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International journal of genomics.

Habib SH, Kausar H, Saud HM. 2016. Plant growth-promoting rhizobacteria enhance salinity stress tolerance in okra through ROS-scavenging enzymes. BioMed research international.

Hanson A. 2015. Evaluating Physiological Responses of Ten Alfalfa (Medicago sativa subsp. falcata) Germplasm to Drought Treatments. The Journal of Undergraduate Research 13, 1-10.

Latrach L, Farissi M, Mouradi M, Makoudi B, Bouizgaren A, Ghoulam C. 2014. Growth and nodulation of alfalfa-rhizobia symbiosis under salinity: electrolyte leakage, stomatal conductance, and chlorophyll fluorescence. Turkish Journal of Agriculture and Forestry 38, 320-326.

López-Gómez M, Hidalgo-Castellanos J, Muñoz-Sánchez JR, Marín-Peña AJ, Lluch C, Herrera-Cervera JA. 2017. Polyamines contribute to salinity tolerance in the symbiosis Medicago truncatula-Sinorhizobium meliloti by preventing oxidative damage. Plant Physiology and Biochemistry 116, 9-17.

Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651-681.

Nelson DW, Sommers LE. 1973. Determination of Total Nitrogen in Plant Material 1. Agronomy Journal 65, 109-112.

Palma F, López-Gómez M, Tejera NA, Lluch C. 2014. Involvement of abscisic acid in the response of Medicago sativa plants in symbiosis with Sinorhizobium meliloti to salinity. Plant Science 223, 16-24.

Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and environmental safety 60, 324-349.

Paul EA. 2014. Soil microbiology, ecology and biochemistry. Academic press.

Putnam DH, Benes S, Galdi G, Hutmacher B, Grattan S. 2017. April. Alfalfa (Medicago sativa L.) is tolerant to higher levels of salinity than previous guidelines indicated: Implications of field and greenhouse studies. In EGU General Assembly Conference Abstracts (Vol. 19, p. 18266).

Rivero RM, Mestre TC, Mittler RON, Rubio F, GarciaSanchez F, Martinez V. 2014. The combined effect of salinity and heat reveals a specific physiological, biochemical and molecular response in tomato plants. Plant, cell & environment 37, 1059-1073.

Roumiantsevaml, Muntyan VS. 2015. Root nodule bacteria Sinorhizobium meliloti: tolerance to salinity and bacterial genetic determinants. Microbiology 84, 303-318.

Sabagh AE, Sorour S, Ragab A, Saneoka H, Islam MS. 2017. The Effect of Exogenous Application of Proline and Glycine Betaineon the Nodule Activity of Soybean Under Saline Condition. Journal of Agriculture Biotechnology 2.

Sheldon AR, Dalal RC, Kirchhof G, Kopittke PM, Menzies NW. 2017. The effect of salinity on plant-available water. Plant and Soil 418, 477-491.

Shrivastava P, Kumar R. 2015. Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi journal of biological sciences 22, 123-131.

Song Y, Nakajima T, Xu D, Homma K, Kokubun M. 2017. Genotypic variation in salinity tolerance and its association with nodulation and nitrogen uptake in soybean. Plant Production Science 20, 490-498.

Tandon HLS, Cescas MP, Tyner EH. 1968. An acid-free vanadate–molybdate reagent for the determination of total phosphorus in soils. Soil Science Society of America Journal 32, 48–51.

Tedersoo L, Brundrett MC. 2017. Evolution of ectomycorrhizal symbiosis in plants. In Biogeography of Mycorrhizal Symbiosis. Springer, Cham p. 407-467.

Wang Y, Zhang Z, Zhang P, Cao Y, Hu T, Yang P. 2016. Rhizobium symbiosis contribution to short-term salt stress tolerance in alfalfa (Medicago sativa L.). Plant and soil 402, 247-261.

Warnke AH, Ruhland CT. 2016. The effects of harvest regime, irrigation, and salinity on stem lignocellulose concentrations in alfalfa (Medicago sativa L.). Agricultural Water Management 176, 234-242.

Alireza Tavasolee, Nader Mirfakhraei, Kazem Khavazi, Hossein Besharati, Hassan Monirifar.
Salt tolerant strains of Sinorhizobium meliloti improved the forage yield and nutrient contents of Medicago sativa under salt toxicity of soil.
J. Bio. Env. Sci. 14(2), 123-130, February 2019.
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