International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Utilization of Bacillus thuringiensis in controlling armyworms (Spodoptera litura) on tomato (Solanum lycopersicum) plants

By: Akhmad Rizali

Key Words: Bacillus thuringiensis, Armyworm, Tomato.

J. Bio. Env. Sci. 14(4), 118-123, April 2019.

Certification: jbes 2019 0147 [Generate Certificate]

Abstract

Tomato plants are horticultural commodities that can provide benefits to farmers, beside the market demand that continues to increase, the cultivation method is easy. Tomatoes are also one type of vegetable plant that contains many vitamins and proteins that have been known by elderly people. Numerous chemical insecticides have been used in order to control pests, which damage for agriculture. While they are too expensive in the developing countries and harmful to both human and the environment. In addition, target insect pests rapidly develop biological resistance especially at higher rates of application. The chemical insecticides are still contributing to human life enormously, but they have been distributed in ecological system of organisms including human beings because of their low specific toxicity to any organism and their low specific toxicity to any organism and their slight decomposition in nature. An alternative control is needed with microbial insecticide which is using B. thuringiensis B. thuringiensis used in this study is B. thuringiensis which is already commercial.Then carried out purification as follows B. thuringiensis concentration of 5g per liter of water, 10g per liter of water, 15g per liter of water, 20g per liter of water. In treatment B. thuringiensis10g per liter of water can stop eating at 2 hours after application, and has been able to control as much as 75 percent.

| Views 60 |

Utilization of Bacillus thuringiensis in controlling armyworms (Spodoptera litura) on tomato (Solanum lycopersicum) plants

Aguskrisno. 2011. Use of Bacillus thuringiensis as a biopesticide. http://education. Kompasian.com. 30 Desember 2011.

Aroson A. 2002. Sorulation and delta-endotoxin synthesis by Bacillus thuringiensis. Cell Mol. Life Sci 59, 417-425.

Asano S, Bando H, Iizuka T. 1993. Amplification and identification of cryII genes from Baccilusthuringiensis by PCR procedures. J. Seric. Sci. Jpn 62, 223-227.

Asano S. 1996. Identification of cry gene from Bacillus thuringiensis by PCR and isolation of unique insecticidal bacteria. Mem. Fac. Agric. Hokkaido Univ 19, 529-563.

Baba F, Asano S, Iizuka T. 1990. Purification of crystals from Bacillus thurigiensisby using Percoll. J. Sci. Jpn 59, 487-489.

Balarman K, Hoti SL, Manonmani LM. 1981. An Idigenous virulent strain of Bacillus thuringiensis, highly pathogenic and specific to mosquitoes. Current Science 50, 199-200.

Boonserm P, Mo, Angsuthana M, Sombat, C, Lescar J. 2006.Structure of the functional form of the mosquito larvicidal cry 4Aa toxin from Bacillus thuringiensis at 2.8-angstrom resolution. J. Bacteriol 188, 3391-3401.

Bourquet S. 2004. Resistance to Bacillus thuringiensis toxin in the Eruropean corn borer: what chance for Bacillus thuringiensis maize?. Physiol. Entomol 29, 251-256.

Bulla LA, Jr, Kramer KJ, Davidson LI. 1977. Characterization of the enmocidalparasporal crystal of Bacillus thuringiensis. J. Bacteriol 130, 375-383.

Delucca AJII, Simonson JG, Larson AD. 1981. Bacillus thuringiensis distribution in soils of the United States.Canadian J. Maicrobiol 27, 865-870.

Dulmage HT. 1992. Insecticidal activity of Bacillus thuringiensis and their potential for pest control in Microbial control for pests and plant diseases and plant diseases 1970-1980 (Ed.H.D Burges). Acad. Press. N.Y. PP.

Golberg LJ, Margalit J. 1977. A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaeniaunguiculata, Culexunivitattus, Aedesaegypti and Culexpipiens, Mosq. New 37, 355-358.

Hasinu JV. 2009. Isolation and B.thuringiensis pathogenicity test against Crocidolomia binotalis Zell. Agriculture cultivation journal 5(2), 84-88.

Hastowo S, Lay BW, Ohba M. 1992. Naturally occurring Bacillus thuringiensis in Indonesia. J. Appl. Bacteriol 73, 108-113.

Heimpel AM. 1967. A critical review of Bacillus thuringiensisBerl.And other crystalliferous bacteria. Ann. Rev. Entomol 12, 287-322.

Held GA, Kawanishi CY, Huang YS. 1990. Characterization of the parasporal inclusion of Bacillus thuringiensis subsp. Kyushuensis. J. Bacteriol. 481-483.

Iizuka T, Ishino M, Nakajima T. 1982. Comparative morphology of Parasporal crystal and characterization of plasmid DNA from various subspecies of entomopathogenic bacteria, Bacillus thuringiensis. J. Fac. Agric. Hokkaido Univ 13, 423-431.

Iizuka T, Sasaki J, Asano S, Bando H. 1995. Comparative studies on isolation and identification of Bacillus thuringiensis. Biotechnology and Enviro. Benefits, Vol I, 143-153.

Iizuka T, Yamamoto T. 1984. Serological properties of the mosquitocidal protein of Bacillus thuringiensis and the morphology of its parasoral crystal. J. fac. Hokkaido Univ 62, 98-114.

Ishii, T, Ohba M. 2013. Investigation of mosquito-specific larvicidal activity of a soil isolate of Bacillus thuringiensi serovar Canadensis. Curr. Microbiol 35, 40-43.

Kalman S, Kiehne KK, Libs JL, Yamamoto T. 1993. Cloning of novel cry IC-type gene from a strain Bacillus thuringiensis subs. Galleriae. Appl. Enviro. Microbio 59, 1131-1137.

Kawalek MD, Benjamin S, Lee HL, Gill SS. 1995.  Isolation and identification of novel toxin from a new mosquitocidal isolate from Malaysia, Bacillus thuringiensis subsp. Jegathesan. Apl. Enviro. Microbiol 2965-2969.

Kim K, Ohba H, aizawa K. 1984. Purification of the toxic protein from Bacillus thuringiensis serotype 10 isolate demonstrating a preferential larvicidal activity to mosquito. J. Invertebr. Pathol. 44, 214-219.

Kreig A, Huger A, Langenbruch G, Schentter W. 1983. Bacillus thurigiensis isolate with activity againtColeoptera. In Biotechnology in invertebrate pathology and cell culture. Karl Maramorosch (ed.) p. 101-114.

Laemmli UK. 1970. Cleavage of structural proteins during tha assembly of the head of bacteriophage T4. Nature (London) 277, 680-685).

Lee SG, Eckblad W, Bulla LA. 1985. Diversity of protein inclusion bodies and identification of mosquitocidal protein in Bacillus thuringiensis  subsp. Israelensis. Biochem. Biophys. Res. Commun 126, 953-960.

Lopez-Pazos SA, Martinez JM, Castilo AX, Samanca JAC. 2009.  Present and significant of Bacillus thuringiensis Cry proteins associated with the Andean weevil Premnotry pesvorax (Coleotera: Curculionidae).

Ohba M, Aizawa K. 1986. Insect toxicity of Bacillus thuringiensis isolated from soils of Japan. J. Invertebr. Pathol 47, 12-20.

Padua LE, Ohba M, Aizawa K. 1984. Isolation of a Bacillus thuringiensis strain (serotype 8a:8b) highly and selectively toxic against mosqouito larvae. J. Invertebr. Pathol 44, 12-17.

Pfannenstiel MA, Ross EJ, Kramer VC, Nickerson KW. 1984. Toxicity and composition of protease-inhibited Bacillus thuringiensis var. israelensiscrystal. FEMS Microbio.Lett 21, 39-42.

Poopathi S, Abidha S. 2010. Mosquitocidal bacterial toxin (Bacillus spaeharicusand Bacillus thuringiensis serovar israelensis): mode of action, cytopathological effects and mechanism of resistance.

Rizali A, Shin-ichiro Asano, Ken Sahara, Hisanori Bando, Bibiana W, Lay, Sugyo Hastowo and Toshihiko Iizuka. 1998. Novel Bacillus thuringiensisserovaraizawai strains isolated from mulberry leaves in Indonesia. Appl. Entomol. Zool 33(1), 111-114.

Rizali A. 2017. Occurrence of Bacillus thuringiensis from different plant areas on South Kalimantan, Indonesia. JBES 11(6), 53-58.

Steinkraus DC, Young SY. 2018. Bacillus thuringiensis for use against armyworm, Pseudaletia unipuncta (Lapidoptera: Noctuidae), On Wheat. Home Vol 82 No. 2.

Syukur M, Helfi ES, Rudi H. 2015. Planting tomatoes in the rainy season. Jakarta.

Akhmad Rizali.
Utilization of Bacillus thuringiensis in controlling armyworms (Spodoptera litura) on tomato (Solanum lycopersicum) plants.
J. Bio. Env. Sci. 14(4), 118-123, April 2019.
https://innspub.net/jbes/utilization-bacillus-thuringiensis-controlling-armyworms-spodoptera-litura-tomato-solanum-lycopersicum-plants/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update