Microbial abundance, physicochemical properties and heavy metals of mangrove swamp water of river Ethiope Sapele, in comparison with the source at Umutu

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Research Paper 11/03/2024
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Microbial abundance, physicochemical properties and heavy metals of mangrove swamp water of river Ethiope Sapele, in comparison with the source at Umutu

Osayamen Collins Udinyiwe, Andrew Edosa Omoregie
Int. J. Micro. Myco.18( 3), 1-14, March 2024.
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Mangrove swamps are distinct and diverse coastal ecosystems located in tropical and subtropical regions. This study was carried out to evaluate the microbial abundance, heavy metals, and physicochemical properties of mangrove swamp water of River Ethiope Sapele, in comparison with the source at Umutu. The microbial isolates were identified using standard microbiological techniques and the values for physicochemical and heavy metal parameters were obtained using standard methods. The total heterotrophic bacteria counts ranged from 5.7±0.9 x 105 cfu/mL to 10.3±1.2 x 105 cfu/mL, while the total fungi counts ranged from 3.3±0.3 x 105 cfu/mL to 5.0±1.5 x 105 cfu/mL. The probable bacterial isolates identified were Pseudomonas aeruginosa, Acinetobacter calcoaceticus, Rhodococcus pneumonia, Bacillus subtillis, Proteus mirabilis, Chromatium okenii, Serratia marcescens, Pseudomonas fluorescens, Escherichia coli. While the probable fungal isolates were Aspergillus niger, Candida utilis., Epicoccum spp., Aspergillus flavus and Rhodococcus glutinis. The Physicochemical analysis carried out revealed values for respective parameters to include pH, ranging from 5.07±0.21 to 6.47±0.39, temperature (26.37±0.09 oC to 26.70±0.51 oC), and BOD (1.08±0.02 mg/L to 2.67±0.16 mg/L). While the heavy metal analysis revealed values to include Cadmium ranging from 0.15±0.03 mg/L to 2.59±0.07a mg/L, Chromium (0.16±0.02 mg/L to 1.23±0.04 mg/L), and Zinc (0.46±0.02 mg/L to 8.47±0.07ab mg/L). Most of the physicochemical and heavy metals parameters analyzed did not fall within the standard values set by World Health Organization for drinking water. This study was able to reveal that there was more pollution at the Sapele axis of River Ethiope than its source at Umutu, due to human activities, including the release of untreated waste into the river from surrounding industries and municipalities.


Abbas S, Ahmed I, Kudo T, Iida, T, Ali GM, Fujiwara T. 2014. Heavy metal-tolerant and psychro-tolerant bacterium Acinetobacter pakistanensis sp. isolated from a textile dyeing wastewater treatment pond. Journal of Agricultural Sciences 51, 593-606.

Abowei JFN, George, ADI. 2009. Some physical and chemical characteristics in Okpoka creek, Niger Delta. Research Journal of Environment and Earth Science 1, 45-53.

Aburto-Oropeza O, Ezcurra E, Danemann G, Valdez V, Murray J. 2008. Mangroves in the Gulf of California increase fishery yields. Proceedings of the National Academy of Sciences of the United State of America 105, 10456–10459.

Adesakin TA, Oyewale AT, Bayero U, Mohammed AN, Aduwo IA, Ahmed PZ, Barje IB. 2020. Assessment of bacteriological quality and physicochemical parameters of domestic water sources in Samaru community, Zaria, Northwest Nigeria. Heliyon 6(8), 4773.

Adeyemi-Ale OA, Aladesida AA., Esenowo IK. 2014. The effect of detergent on the physicochemical characteristics and plankton diversity of Osere stream, Ilorin, Kwara, Nigeria. Journal of Applied Science and Environmental Management 18(1), 109-113.

Adjarho UB, Esenowo IK, Ugwunba AAA. 2013. Physico-chemical parameters and micro-invertebrate fauna of Ona River at Oluyole Estate, Ibadan, Nigeria. Research Journal of Environmental and Earth Science 5(1), 671-676.

Ajuzie CU, Osaghae RA. 2011. Bacteriological and physicochemical properties of hair salon waste water and contaminated soil in Benin metropolis. African Journal of Biotechnology 10(11), 2066-2069.

Akankali JA, Davies IC, Saro-Wiwa PB. 2020. Impact of Abattoir Waste on the Water Quality of Okpoka Creek, Niger Delta. Journal of Wetland and Waste Management 4(1), 56-65

Akatah HA, Omonigho S, Ibrahim AGG, Seyinde DO. 2018. Physicochemical and microbiological analysis of Ikpoba River Water in Benin City, Edo State, Nigeria. International Research Journal of Biological Sciences 7(5), 1-6.

Arimoro FO, Iwegbue CMA, Osiobe O. 2018. Effects of industrial waste water on the physical and chemical characteristics of a tropical coastal river. Resource Journal of Environmental Sciences 2, 209-220.

Asibor G, Edjere O, Ebighe D. 2016. Leachate characterization and assessment of surface and groundwater water qualities near municipal solid waste dumpsite at Okuvo, Delta State, Nigeria. Ethiopian Journal of Environmental Studies and Management 9(4), 523-533.

Association of Official Analytical Chemist (A.O.A.C). 2012. Official Methods of Analysis. (19th edition) Washington, DC. 121-130p.

Atuanya EI, Iserhienrhien GO, Emejulu MJ. 2023. Effect of produced formation water on soil nitrifying bacteria and earthworms. Journal of Applied Science and Environmental management 27(4), 869-876.

Azubuike CC, Chikere CB, Okpokwasili GC. 2016. Bioremediation techniques-classification based on site of application: Principles, advantages, limitations and prospects. World Journal of Microbiology and Biotechnology 32, 180-185

Barry DA, Frick P.J, Grooms T. 2000. Analytical approximations for real values of the Lambert W-Function. Mathematics and computers in simulation 53, 95-103.

Ben-Coker MO. 2012. Effect of slaughter house discharge on water quality of Ikpoba River, Nigeria. Journal of Bioscience Technology 52(1), 1-4.

Cheesebrough M. 2000. District Laboratory Practices in Tropical Countries (2nd edition) UK: Cambridge, Cambridge University Press

Cheesebrough M. 2006. District Laboratory Practices in Tropical Countries (2nd edition) London: Cambridge University Press. 62p.

Chikere CB, Okpokwasili GC, Ichiakor O. 2009. Characterization of hydrocarbon utilizing bacteria in tropical marine sediments. African Journal of Biotechnology 8(11), 541-2544.

Chitmanat C, Traichaiyaporn S. 2010. Spatial and temporal variations of physicochemical water quality and some heavy metals in water, sediments and fish of the Mae Kuang River, Northern Thailand. International Journal of Agriculture and Biology 12(6), 816-820.

Deborah JM, Dammo MN, Watila S, Sangodoyin AY. 2017. Impacts of effluents on River Nggada water quality in Maiduguri, Nigeria. Advancement in Scientific and Engineering Research 3(1), 1-9.

Deng SJ, Wang K, Hong Y, Qi GJ. 2009. Spatio-temporal dynamics and evolution of land use change and landscape pattern in response to response to rapid urbanization. Landscape Urban Planning 92, 187-198.

Duke NC, Meynecke JO, Dittmann S, Ellison AM, Anger A. 2007. World without Mangroves. Science 317, 41–42.

Edjere O, Ibezute AC, Ibezute SU. 2016. Impact of Brewery Factory Effluent on the Physicochemical Characteristics of Ikpoba River in Edo State, Nigeria. Resources and Environment 6(3), 53-62.

Edokpayi CA, Osimen EC. 2001. Hydrobiological studies on Ibiekuma River at Ekpoma, southern Nigeria, after impoundment: the faunal characteristics. African journal of Science and Technology 2(1), 72-81.

Ehiagbonare JE, Enabulele SA, Babatunde BB, Adjarhore R. 2009. Effect of cassava effluent on okada denizens. Scientific Research and Essay 4(4), 310-313.

El-Deek AA, El-Deen MS, Hamdy SM, Asar MA, Yakout HM, Attia YA. 2013. Effect of different dietary levels of NaCl and KCl on the performance of broiler chicks fed plant diets. Egypt Poultry Science 29, 907-921.

Eneji IS, Aguda PO, Sha’ato R. 2012. Spatial and temporal variation in water quality of River Benue, Nigeria. Journal of Environmental Protection 3, 1-7.

Enemuor SC, Victor PI, Oguntibeju OO. 2012. Microbial contamination of currency counting machines and counting room environment in selected commercial banks. Scientific Research and Essay 7(14), 1508-1511.

Ezekiel EN, Hart AI, Abowei JFN. 2011. The physical and chemical condition of Sombreiro River, Niger Delta, Nigeria. Research journal of environment and Earth Science 3(4), 327-340.

Falkowski PG, Fenchel T, Delong EF. 2008. The microbial engines that drive Earth’s biogeochemical cycles. Science 320, 1034-1039.

Gaherwar S, Kulkarni P. 2012. Studies on removal of toxic heavy metals from water by Eichhornia crassipes. Indian Journal of Science and Research 3(2), 95-103.

Gulla S, Waghray K. 2011. Effect of storage on physicochemical characteristics and fatty acid composition of selected oil blends. Journal of Lipid Science 3(1), 35-46.

Hou LJ, Liu M, Xu SY, Ou DN, Yu J, Cheng SB, Lin X, Yang Y. 2007. The effects of semi-lunar spring and neap tidal change on nitrification, denitrification, and N2O vertical distribution in the intertidal sediments of the Yangtze estuary, China. Estuary Coastal Shelf Science 73, 607–616.

Hu B, Liao J, Zhang Q, Ding S, He M, Qiao Y, Zhang Z, Shang C, Chen S. 2022. Diversity and vertical distribution of sedimentary bacterial communities and its association with metal bioavailability in three distinct mangrove reserves of South China. Water 14, 971-974.

Ibiene AA, Agbeyi EV, Okonko IO. 2012. Bacteriological assessment of drinking water sources in Opuraja community of Delta State, Nigeria. Nature and Science 10(1), 36-41.

Ikomi RB, Arimoro FO, Odihirin OK. 2005. Composition, Distribution and Abundance of Macroinvertebrates of the Upper reaches of River Ethiope, Delta State, Nigeria. Zoologist 3, 68-81.

Iloba KI. 2012. The physicochemical characteristics and plankton of Ethiope River, Nigeria. Ph.D Thesis, Delta State University Abraka, Delta State. 444p.

Iwegbue CMA, Eghwrudje MO, Nwajei GE, Egboh SHO. 2007. Chemical speciation of heavy metals in the Ase River sediments, Niger Delta, Nigeria. Chemical Speciation and Bioavailability 19(13), 119-129.

Kolawole F., Akinwande BA, Ade-Omowaye BI. 2020. Physicochemical properties of novel cookies produced from orange fleshed sweet potato cookies enriched with sclerotium of edible mushroom (Pleurotus tuberregium). Journal of the Saudi Society of Agricultural Sciences 19(2), 174- 178.

Kwadzah TK, Iorhemen OT. 2015. Assessment of the Impact of Abattoir Effluent on the Water Quality of River Kaduna, Nigeria. World Journal of Environmental Engineering 3(3), 87-94.

Liu M, Huang H, Bao S, Tong Y. 2019. Microbial community structure of soils in Bamenwan mangrove wetland. Science Republican 9, 8406-8410.

Masese FO, Raburu PO, Mwasi BN, Etiegni L. 2011. Effects of Deforestation on Water Resources: Integrating Science and Community Perspectives in the Sondu-Miru River. 1-14p.

McMichael AJ, Kjellstrom T, Smith K. 2001. Environmental health. In International Public Health. Merson MH, Black RE, Mills AJ (Eds.). Gaithersburg. 379-438p.

Milićević D, Niksić M, Baltić T, Vranić D. 2010. Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms. World Journal of Microbiology and Biotechnology 26, 1715-1720.

Morrison G, Fatoki OS, Persson L, Ekberg A. 2001. Assessment of the impact of point source pollution from the Keiskammahoeh Sewage Treatment Plant on the Keiskamma river: pH, electrical conductivity, oxygen demanding substance (COD) and nutrients, Water SA 27, 75-78.

Nwaugo VO. 2008. Impact of cassava mill effluent (CME) on soil physiochemical and microbial community structure and functions. Nigerian Journal of Microbiology 22(1), 1681–1688.

Odekina MU, Davies IC, Akoko S, Vincent-Akpu, IF. 2021. Bioaccumulation of Heavy Metals in Periophthalmus papillio, Sediment and Interstitial Water from Isaka-Bundu Water Front in Rivers State. Academic Journal of Current Research 8(11), 19-38.

Ogbeibu, AE, Omoigberale MO. 2005 Environmental impacts of oil exploration and production on the rotifers of Osse River, Southern Nigeria. African Journal of Environmental Pollution and Health 4, 72-80.

Ogbeibu 2005. Biostatistics: A Practical Approach to Research and Data Handling. Mindex Publishing Company Limited. Benin city, Edo State. 264p.

Ogboghodo IA, Oluwafemi AP, Ekeh SM. 2006. Effects of polluting soil with cassava mill effluent on the bacteria and fungi populations of a soil cultivated with maize. Environmental Monitoring and Assessment 116, 419-425

Oluyemi EA, Adekunle AS, Adenuga AA, Makinde W.O. 2010. Physico-chemical properties and heavy metal content of water sources in Ife North Local Government Area of Osun State, Nigeria. African Journal of Environmental Science Technology 4(10), 671-697.

Onojake MC, Sikoki FD, Babatunde BB, Akpiri RU, Akpuloma D, Omokheyeke O. 2015. Bioaccumulation of heavy metals in two matrices of the Bonny/New Calabar River Estuary in Niger Delta, Nigeria. Ocean Science Journal 50(2), 203-208.

Onojake MC, Ukerun SO, Iwuoha GA. 2011. Statistical approach for evaluation of the effects of industrial and municipal wastes on Warri Rivers, Niger Delta, Nigeria. Water Quality, Exposure, and Health 3(2), 91-99.

Osuji LC, Onojake CM. 2006. Field reconnaissance and estimation of petroleum hydrocarbon and heavy metal contents of soils affected by the Ebocha-8 oil spillage in Niger Delta, Nigeria. Journal of environmental Management 79(2), 133-139.

Pahalawattaarachchi V, Purushothaman CS, Vennila A. 2009. Metal phytoremediation potential of Rhizophora mucronata (Lam.). Indian Journal of Marine Sciences 38(2), 178–83.

Salisu MA, Noordin W, Sulaiman Z, Hilim RA. 2017. Influence of soilless potting media on growth and vegetative traits of immature rubber (Hevea brasiliensis). Bangladesh Journal of Botany 46(1), 451-457.

Scott WS, Pepper IL, Gerba CP. 2005. Contribution of drinking water to the weekly intake of heterotrophic bacteria from diet in the United States. Water Research 39(1), 257-263.

Shaikh K, Gachal GS, Memon SQ, Ahmed N, Sodho MYS. 2014. Assessment of amphibian environment through Physicochemical analysis in Pakistan 28p.

Thatoi H, Behera BC, Mishra RR, Dutta SK. 2013. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: A review. Ann Microbiology 63, 1–19.

Udochukwu U, Atuanya EI, Abidemi JM. 2017. Ecological effects of plastic composed water on soil biological sentinels. Nigerian Journal of Scientific Research 16(1), 1- 3

Udotong IR, Udotong JIR, John OUM. 2015. Delineation of oil-polluted sites in Ibeno LGA, Nigeria, using Geophysical techniques. International Journal of Environmental Chemical, Ecological 9(6), 617-623.

United States Environmental Protection Agency (USEPA). 2012. Recreational Water Quality Criteria. Quality Assurance Guidance 20, 12-58.

Upadhyay VP, Ranjan R, Singh JS. 2002. Human-mangrove conflicts: the way out. Current Science 83(11), 1328–1336.

Usoro E, Enewan U, Thomas AH. 2013. Seasonal variation of physicochemical parameters of water and sediments from Iko River, Nigeria. Journal of Environmental and Earth Sciences 3(8), 96-110.

Walters BB, Ronnback P, Kovacs JM, Crona B, Hussain SA, Badola R, Primavera JH, Barbier E, Dahdouh-Guebas F. 2008. Ethnobiology, socioeconomic and management of mangrove forests: A review. Aquatic Botany 89(2), 220–36.

World Health Organization (WHO). 2000. Guidelines for Drinking Water Quality (2nd Edition). Geneva

World Health Organization (WHO). 2009. Guidelines for Drinking Water Quality (4th Edition). Geneva

World Health Organization (WHO). 2011.  Guidelines for Drinking Water Quality (4th edition). Switzerland: WHO Library Cataloguing-in-Publication

Wu J, Guo Y, Liu Y, Chen F. 2009. Rapid determination of oil content in seed of Jatropha curcas by NMR. Journal of Biobased Material and Bioenergy 4, 436-439.

Wu Q, Li HR, Wang J, Paulussen Y, He M, Wang B, Wang Z. 2006. Monitoring and Predicting Land use change in Beijing using Remote sensing and GIS. Landscape and Urban Planning 78, 322-333.

Yakasai IA, Salawu F, Musa H. 2004. Concentrations of lead in Ahmadu Bello University dam, raw, treated (Tap) and Abucons pure waters. Chemical Class Journal 1, 86-90.

Zhang Z, Qu C, Zhang K. 2020. Adaptation to Extreme Antarctic Environments Revealed by the Genome of a Sea Ice Green Alga. Current Biology 30, 3330-3341.