Nasal microflora of marginalized adult populace in residential proximity to an abandoned dumpsite in Iligan City, Philippines
Paper Details
Nasal microflora of marginalized adult populace in residential proximity to an abandoned dumpsite in Iligan City, Philippines
Abstract
Nasal swab cultures of seventy adults who are proximal residents of the abandoned city dumpsite were collected in order to determine the colonizing microflora of their anterior nares. Nasal bacterial colonization rates of microorganisms were greater during wet season, which might be due to proliferation of microorganisms from fecal matter that were left behind when there is a hard rain wash. Conventional methods were employed for the morphological (growth patterns in nutrient agar plate, slant and nutrient broth) and cellular (Gram stain, simple sporulation stain and acid-fast stain) characterization of the 214 bacterial strains that were isolated for their preliminary classification. Physiological characterization was done through catalase and coagulase tests, blood agar hemolysis, mannitol salt and glucose fermentation reactions. The presumptively identified bacterial strains were Staphylococcus aureus (79), coagulase negative staphylococci (17), Streptococci (17), Corynebacterium (32), Bacillus (24), Micrococcus luteus (14), Mycobacterium (4) and Lactobacillus (27).
Abul S. 2010. Environmental and Health Impact Of Solid Waste Disposal At Mangwaneni Dumpsite In Manzini: Swaziland. Journal of Sustainable Development in Africa 12(7), 64-78.
Benson HJ. 2001. Microbiological Applications, Laboratory Manual in General Microbiology. 8th ed. The McGraw-Hill Companies, Inc. NY.
Carbohydrate Fermentation Test: Uses, Principle, Procedure and Uses. 2016. https://microbeonline.com/carbohydrate-fermentation-test-uses-principle-procedure-results/
Feldman C, Anderson R. 2013. Cigarette smoking and mechanisms of susceptibility to infections of the respiratory tract and other organ systems. Journal of Infection 67(3), 169-184. https://doi.org/10.1016/j.jinf.2013.05.004
Heikkinen T, Marttila J, Salmi AA, Ruuskanen O. 2002. Nasal Swab versus Nasopharyngeal Aspirate for Isolation of Respiratory Viruses. Journal of Clinical Microbiology 40(11), 4337–4339. http://dx.doi.org/10.1128/JCM.40.11.43374339.2002
Johnson MT. 2001. Hemolysis on Blood Agar. Medical Microbiology J621/LIFS675.
Kaiser GE. 2002. Staphylococcus aureus Growing on Mannitol Salt Agar. Doc Kaiser’s Microbiology.
Moreth-Kebernik U, Schmidt O. 1991. Identification of indoor rot fungi by taxon-specific priming polymerase chain reaction. Holzforschung 54, 1-8.
Nguyen DM, Mascola L, Bacroft E. 2001. Recurring Methicillin-resistant Staphylococcus aureus in Infections in a Football Team. Emerging Infectious Diseases 11(4).
Pukkala E, Ronka A. 2001. Increased Incidence of Cancer and Asthma in Houses Built on a Former Dump Site. Environmental Health Perspectives 109(11), 1121-1125.
Rafii F, Sutherland JB, Cerniglia CE. 2008. Effects of treatment with antimicrobial agents on the human colonic microflora. Therapeutics and Clinical Risk Management 4(6), 1343-1358.
Rasmussen TT, Kirkeby LP, Poulsen K, Reinholdt J, Kilian M. 2008. Resident aerobic microbiota of the adult human nasal cavity. Journal of Pathology, Microbiology and Immunology 108(10), 663-665. https://doi.org/10.1034/j.1600-0463.2000.d01-13.x
Reynolds J. 2012. Lab Procedures Manual. http://delrio.dcccd.edu/jreynolds/microbiology/2421/lab_manual/TOC.html
Ray MR, Mukherjee G, Roychowdhury S, Lahiri T. 2004. Respiratory and General Health Impairments of Rag Pickers in India: A Study in Delhi. International Archives of Occupational and Environmental Health 77(8), 595-598. http://dx.doi.org/10.1007/s00420-004-0564-8
Lucilyn L. Maratas, Ermee Gild S. Calumba, 2018. Nasal microflora of marginalized adult populace in residential proximity to an abandoned dumpsite in Iligan City, Philippines. Int. J. Biosci., 13(4), 230-236.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.