Phytochemical study of three endemic brooms in Algeria: Genista numidica Spach, Genista ferox Poiret and Genista tricuspidata Desf

Paper Details

Research Paper 01/09/2017
Views (226) Download (7)
current_issue_feature_image
publication_file

Phytochemical study of three endemic brooms in Algeria: Genista numidica Spach, Genista ferox Poiret and Genista tricuspidata Desf

Samira Ati, Bennadja Salima, Boumaraf Warda
Int. J. Biosci.11( 3), 178-183, September 2017.
Certificate: IJB 2017 [Generate Certificate]

Abstract

Genista numidica, Genista ferox and Genista tricuspidata are three endemic plants belonging to the family Fabaceae and which remain very little studied. The purpose of this work is to evaluate the antioxidant power of methanolic extracts of the flowers and the leaves of these species according to the DPPH free radical scavenging method while determining their total phenol, total flavonoids and tannin contents (the secondary metabolite), Flavonoids were estimated by the aluminum chloride (AlCl3) method, the total phenols were determined with Folin-Ciocalteu reagent and the tannins were estimated by the vanillin in acid medium method. The investigations showed that G. numidica is the most rich in polyphenols (113 ± 3.87mg and 79.6 ± 3.49 mg) of gallic acid equivalent and it’s the most flavonoid-rich with 85.47 ± 1.51mg/g and 87.31mg/g of quercetin equivalent in flowers and leaves  respectively.  However, G. ferox was the most-rich in tannins in both leaves and flowers with 39.81 ± 0.32 mg/g and 44.36 mg /g of catechin equivalent respectively.  G. tricuspidata showed a high level in the flowers extract (39.36 ± 0.32mg / g) and G.numidica gave 37.77 ± 0.64mg / g in the leaves extract. The antioxidant activity was the best in leaves and flowers extracts of G. ferox (IC50 = 0.50 ± 0.006 mg/ml and 0.59 ± 0.02mg /ml) as well as G.numidica leaf extract (0.55 ± 0.03 mg /ml). These results showed that this species  would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds.

VIEWS 12

Benhamou N. 2011. Antioxidant activity of extracts of the phenolic compounds of ten medicinal plants in the West and Southwest of Algeria, PhD thesis in biology, Tlemcen, Algérie, P 54. 

Bolland GM, Donnely DMX. 1998. Isoflavonoids and Related Compounds, journal Natural Product Reports 15, 241-260. https://doi.org/10.1039/A815241Y.

Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology 28. 25-30.18. https://doi.org/10.1016/S0023-6438(95)80008-5

Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M and Abdelly C. 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. Compte Rendue Biologies. 331, 372-379. https://doi.org/10.1016/j.crvi.2008.02.008

Ghasemzadeh A, Jaafar Hawa ZE, Rahmat A. 2011. Effects of solvent type on phenolics and flavonoids content and antioxidant activities in two varieties of young ginger (Zingiber officinale Roscoe) extracts. Journal of Medicinal Plants Research.; 5(7): 1147-1154. ISSN 1996-0875 ©2010 Academic Journals. http://www.academicjournals.org/JMPR

Giachi I, Manunta A., Morelli I, Pistelli L. 2002.  Flavonoîds and isoflavonoîds from Genista morisii, Biochimical systematics and Ecology 30. 801-803. https://doi.org/10.1016/S0305-1978(01)00116-8

Halliwell B, Gutteridge J. 1999. The Free radicals in biology and medicine, Oxford  Oxford University Press 2015.

Julkunen-Titto R. 1985. Phenolic constituents in the leaves of northern willows : methods for the analysis of certain phenolics. Journal of Agricultural and Food chemistry, 33, 213-217. https://doi.org/10.1021/jf00062a013.

Kirch J, Vert M, Watzingh H, Greinwald R, Grygan FC. 1995. AlKaloîdal variation in Genista lobelii S.I (Fabaceae), Biochemical systematic and Ecology vol 23 ,N°6, 635-643 p. https://doi.org/10.1016/0305-1978(95)00037-2

Ordon Ez AAL, Gomez JD, Vattuone MA, Isla MI. 2006. Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chemistry 97, 452-458 https://doi.org/10.1016/j.foodchem.2005.05.024.

Pistelli L, Bertoli A, Giachi I, Morelli I, Rubiolo P, Bicchi C. 2001. Quinolizidine alkaloids from Genista ephedroide, biochemical and systematic and Ecology 29. 137-141p.183 https://doi.org/10.1016/S0305-1978(00)00046-6

Quezel P, Santa. 1962 et 1963 .Nouvelle flore de l’Algérie et des région désertiques méridionales.Tome I .C.N.P.S.Paris.

Singleton VL, Orthofer R, Lamuela- Raventos RM. 1999. Analysis of total phenols and other antioxidants by means of Folin- Ciocalteu reagent 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1

Sanchez-Moreno C. 2002. Methods used to evaluate the free radical scavenging activity in foods and biological systems International Journal of Food Science and Technology 8, 121-137. https://doi.org/10.1106/108201302026770