Screening of Lipote (Syzigium currannii) pure fruit extract for use as cheek cell and onion cell stain
Paper Details
Screening of Lipote (Syzigium currannii) pure fruit extract for use as cheek cell and onion cell stain
Abstract
The effectiveness of lipote fruit (Syzygium currannii) pure extract for use in staining cheek cells and onion cells were studied to obtain non-toxic, environmentally friendly, and low-cost dyes for use in staining. Syzygium currannii is a small to medium-sized tree growing up to 14 meters tall. The fruit extract of Lipote has anthocyanins which are categorized as ternatins. A number of histological techniques have been identified to be used to provide a nuclear stain consisting of natural phenolic compounds, structurally related to anthocyanins. The objective of this study was to determine the effectiveness of lipote fruit extract on cheek cell and onion cell staining. Dye extracts from Syzygium currannii were used to stain cheek cells and onion cells using the existing standard staining procedures with little modification. The prepared extracts had an affinity for the cell membrane and nucleus. From the results of the Mann- Whitney U test, the stains had significant results at a P-value of 0.001413 as the highest. Therefore, this study shows that dye extracts from Syzygium currannii could be used for cheek cell and plant cell staining. Results of the stain on onion cells have the most significant result compared with the cheek cell stain results.
Chacon N. 1995. Phytochemical, microbiological and pharmacological screening of the alcoholic extract from the bark of lipote (Syzygium polycephaloides) C.B. Rob (Family Myrtaceae)/ Centro Escolar Univ., Manila, Mar.
Chew Weng C, Abdullah R, Saad S. 2014. Alternative staining using extracts of hibiscus (Hibiscus rosa-sinensis L.) and red beet (Beta vulgaris L.) in diagnosing ova of intestinal nematodes (Trichuris trichiura and Ascaris lumbricoides) 1, 14-18
Khoo HE, Azlan A, Tang ST, Lim SM. 2017. Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food & nutrition research 61(1), 1361779. https://doi.org/10.1080/16546628.2017.1 9
Macfoy C. 2004. Ethnobotany and Sustainable Utilization of Natural Dye Plants in Sierra Leone. Economic Botany 58, S66-S76.10.1663/00130001 (2004)58[S66:EASUON]2.0.CO;2.
Nguyen D. 2017. What Is the Advantage of Using Stains to Look at Cells? Sciencing.com. Copyright 2017 Leaf Group Ltd. https://sciencing.com /advantage-using-stains-look-cells-19317.html
Onslow M. 2013. The Anthocyanin Pigment of Plants. Google Books. Page 32. Retrieved from: books.google.com.ph/books
Rosemary B, Abraham A, Ademola A. 2012. Staining effect of Hibiscus sabdariffa extract on sperm cell morphology of Sprague-Dawley rats, Journal of Histotechnology 35(3), 110-113 (2012)
Santiago D, Garcia V, Dizon E, Merca F. 2007. Antioxidant Activities, Flavonol and Flavanol Content of Selected Southeast Asian Indigenous Fruits. Philippine Agricultural Scientist 90(2), 123-130
Suebkhampet J, Pongsiwa A. 2012. Effect of using aqueous crude extract from butterfly pea flowers (Clitoria ternatea L.) As A dye on animal blood smear staining. Suranaree J. Sci. Technol 19(1), January – March 2012
Ma Chrischelle F. Bullecer, Romnick Z. Hidalgo (2022), Screening of Lipote (Syzigium currannii) pure fruit extract for use as cheek cell and onion cell stain; JBES, V20, N5, May, P52-56
https://innspub.net/screening-of-lipote-syzigium-currannii-pure-fruit-extract-for-use-as-cheek-cell-and-onion-cell-stain/
Copyright © 2022
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0