home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Efficacy of different essential oils, fungicides and biocontrol agents against Aspergillus niger the causal agent of fruit rot in Pomegranate

Paper Details

Research Paper 01/03/2020
Views (495) Download (26)
current_issue_feature_image
publication_file

Efficacy of different essential oils, fungicides and biocontrol agents against Aspergillus niger the causal agent of fruit rot in Pomegranate

Ghulam Hussain Jatoi, Shar Muhammad, Wazir Ali Metlo, Laith Khalil Tawfeeq Al-Ani, Haseenullah, Manzoor Ali Abro, Muswar Ali Gadhi, Naveed Wahid Awan, Manzoor Ahmed Reki
Int. J. Biosci.16( 3), 51-65, March 2020.
Certificate: IJB 2020 [Generate Certificate]
Key: Aspergillus nigerBiocontrolEssential oilsFungicidesPomegranate

Abstract

Different essential oils, fungicides and biocontrol agents against Aspergillus niger the causal agent of fruit rot in pomegranate. The importance of survey and sampling were done and the pathogenicity test against Aspergillus niger was performed. The antifungal potential of different essential oils like Laung, Turpentine, Castus root, Neem, Gulab and Khashkhas was carried out at different doses i.e. 5% 10% and 15% find out the effective oil for the growth inhibition of Aspergillus niger effect of some fungicides viz., Melodyduo, Topsin-M, Prevail, Antracol, and Cabriotop against the causal pathogen by food poisoning method at 3 different concentration (100, 200, 300 ppm). Disease incidence was recorded in Killi Oryagi (40%) followed by Killi Murtath (20%), Killi Pattankot (18%) and Killi Zangiwal (14%). Minimum disease incidence was recorded in Killi Lashti (8%). Injection method of inoculation showed a higher percentage of rotting (7.0%) as compared to the cut method of inoculation (4.05%). Minimum colony growth of Aspergillus niger (0.10, 0.20 and 0.30%) examined Laung at the dosage Turpentine (57.33, 45.52 and 25.13%), Gulab (41.50, 35.50 and 29.50%), Castus root (65.57, 44.45 and 32.96%), Neem oil (45.00, 42.00 and 37.00%) Maximum colony growth of Aspergillus niger (49.00, 45.00 and 41.00%) was observed Minimum linear colony growth of Aspergillus niger as observed for Prevail (13.20, 4.72 and 0.25%) at various concentrations respectively followed by Topsin-M (28.50, 20.50 and 11.50%), Cabriotop (33.00, 26.50 and 13.50%), Antracol (49.84, 33.54 and 21.96%), Alliete (44.09, 32.65 and 22.83%) and maximum growth of fungus were determined under Melodyduo (57.16, 45.31 and 37.42%). The fungus growth was observed up to 90% under control.

VIEWS 30
Cover PDF

Aly K, Shaukat SS, Mian S. 2011. Management of plant nematodes associated with pomegranate (Punica granatum L.) using oil-cakes in Balochistan, Pakistan. Indian Journal of Nematology 41, 1-3.

Bardas G, Tzelepis G, Lotos L, Karaoglanidis G. 2009. First report of Botrytis cinerea causing gray mold of pomegranate (Punica granatum) in Greece. Plant disease 93, 1346.

Bharani RA, Namasivayam SKR. 2016. Pomegranate (Punica granatum L.) Peel Extract-A Study On Potential Source Of Pharmacological Activities. International Journal of Pharma and Bio Sciences 7, 282-90.

Cavanagh H, Wilkinson J. 2002. Biological activities of lavender essential oil. Phytotherapy research 16, 301-8.

Chami F, Chami N, Bennis S, Bouchikhi T, Remmal A. 2005. Oregano and clove essential oils induce surface alteration of Saccharomyces cerevisiae. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 19, 405-8.

Da Silva JaT, Rana TS, Narzary D, Verma N, Meshram DT, Ranade SA. 2013. Pomegranate biology and biotechnology: a review. Scientia Horticulturae 160, 85-107.

Dorman H, Deans SG. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88, 308-16.

Gross PM. 2007. Superfruits take center state. NPI Center. Retrieved October 17, 2007.

Guynot M, Ramos A, Seto L, Purroy P, Sanchis V, Marin S. 2003. Antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. Journal of Applied Microbiology 94, 893-9.

Hebert T, Clayton C.  1963. Pomegranate fruit rot caused by Coniella granati. Plant Disease Reporter 47, 222-3.

Inouye S, Tsuruoka T, Watanabe M. 2000. Inhibitory effect of essential oils on apical growth of Aspergillus fumigatus by vapour contact. Mycoses 43,  17-23.

Inouye S, Uchida K, Abe S. 2006. Vapor activity of 72 essential oils against a Trichophyton mentagrophytes. Journal of Infection and Chemotherapy 12, 210-6.

Jain K, Desai N. 2018. Pomegranate the cash crop of India: a comprehensive review on agricultural practices and diseases. International J Health Science Res 8, 315-36.

Jamadar M, Jawadagi R, Sataraddi A, Patil D, Patil R. 2011. Status of pomegranate diseases of northern Karnataka in India. Acta horticulturae 890, 501-7.

Jeff-Agboola Y, Onifade A, Akinyele B, Osho I. 2012. In vitro antifungal activities of essential oil from Nigerian Medicinal Plants against toxigenic Aspergillus flavus. Journal of Medicinal Plants Research 6, 4048-56.

Li Y, Gao F, Gao F, Shan F, Bian J, Zhao C, 2009. Study on the interaction between 3 flavonoid compounds and α‐amylase by fluorescence spectroscopy and enzymatic kinetics. Journal of Food Science 74, C199-C203.

Mirabolfathy M, Groenewald J, Crous P, 2012. First report of Pilidiella granati causing dieback and fruit rot of pomegranate (Punica granatum) in Iran. Plant Disease 96, 461.

Moller W, Devay J. 1968. Carrot as a species-selective isolation medium for Ceratocystis fimbriata. Phytopathology 58, 123-4.

Munhuweyi K, Lennox CL, Meitz-Hopkins JC, Caleb OJ, Opara UL. 2016. Major diseases of pomegranate (Punica granatum L.), their causes and management—A review. Scientia Horticulturae 211, 126-39.

Pawar V, Thaker V. 2006. In vitro efficacy of 75 essential oils against Aspergillus niger. Mycoses 49, 316-23.

Pina‐Vaz C, Gonçalves Rodrigues A, Pinto E. 2004. Antifungal activity of Thymus oils and their major compounds. Journal of the European Academy of Dermatology and Venereology 18, 73-8.

Pitt JI, Hocking AD. 1997. Aspergillus and related teleomorphs. In. Fungi and food spoilage. Springer, 339-416.

Rajendiran R, Jegadeeshkumar D, Sureshkumar B, Nisha T. 2010. In vitro assessment of antagonistic activity of Trichoderma viride against post harvest pathogens. Journal of Agricultural Technology 6, 31-5.

Remmal A, Bouchikhi T, Rhayour K, Ettayebi M, Tantaoui-Elaraki A. 1993. Improved method for the determination of antimicrobial activity of essential oils in agar medium. Journal of Essential Oil Research 5, 179-84.

Sabulal B, Dan M, Kurup R, Pradeep NS, Valsamma RK, George V. 2006. Caryophyllene-rich rhizome oil of Zingiber nimmonii from South India: Chemical characterization and antimicrobial activity. Phytochemistry 67, 2469-73.

Sharma N, Jain A. 1978. Two new fruit rot diseases of pomegranate (Punica granatum L.) caused by Coniella spp.[India]. Current Science (India).

Snowdon AL. 1990. A colour atlas of post-harvest diseases and disorders of fruits and vegetables.

Suhr KI, Nielsen PV. 2003. Antifungal activity of essential oils evaluated by two different application techniques against rye bread spoilage fungi. Journal of Applied Microbiology 94, 665-74.

Tantaoui-Elaraki A, Beraoud L. 1994. Inhibition of growth and aflatoxin production in Aspergillus parasiticus by essential oils of selected plant materials. Journal of environmental pathology, toxicology and oncology: official organ of the International Society for Environmental Toxicology and Cancer 13, 67-72.

Thomidis T, Exadaktylou E. 2011. First report of Pilidiella granati on pomegranate with symptoms of crown rot in the prefecture of Xanthi, Greece. Plant disease 95, 79.

Tomás-Barberán FA, Ruiz D, Valero D. 2013. Health benefits from pomegranates and stone fruit, including plums, peaches, apricots and cherries. Bioactives in Fruit: Health Benefits and Functional Foods, 125-67.

Submit Manuscript