Amelioration of myocardial apoptosis by using Ocimum basilicum in rats after exposure to electromagnetic field (EMF): light and transmission microscopic study

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Research Paper 15/10/2012
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Amelioration of myocardial apoptosis by using Ocimum basilicum in rats after exposure to electromagnetic field (EMF): light and transmission microscopic study

Amir Afshin Khaki, Arash Khaki
Int. J. Biosci.2( 11), 1-10, October 2012.
Certificate: IJB 2012 [Generate Certificate]

Abstract

Apoptotic cell death plays a pivotal role in the development of heart failure. Exposure to EMF that arises from electronic means has harmful effect on public health, and cause to cell injury like apoptosis Medicinal use of ocimum basilicum dates back to ancient Iran, China and India. It has been used since ancient time as medicinal and food origin as an antioxidant’s ocimum basilicum has a useful effect on many tissues as a protective on emf harmful Sid effects.Wistar male rat (n=40) were allocated into four groups, control (n=10) and test groups (n=30), that subdivided into groups of 3, the extract group were received of ocimum basilicum extract (1.5g/kg body), second extract group were received of ocimum basilicum extract (1.5g/kg body) and emf group that exposed to50 Hz for 40 consequence day. for 40 consequence day. Animals were kept in standard conditions. In end of study the heart tissue of Rats in whole groups were removed and prepared for pathology and biochemical analysis. Serum MDA, percentage of apoptotic cells and artery hyperemia significantly were increased in experimental group that has exposed to 50Hz EMF (p<0.05).the level of TAC, GPX in groups which received 1.5g/kg body ocimum basilicum extract significantly were increased (p<0.05) in comparison to control group. Morphology of heart in both experimental and control group were similar. Results revealed that administration of 1.5g/kg body of ocimum basilicum extract significantly decreased the apoptotic rate and protects myocardial cells by presents its antioxidant role.

VIEWS 9

Andrzejak R, Poreba R, Poreba M, Derkacz A, Skalik R, Gac P, Beck B, Steinmetz-Beck A, Pilecki W. 2008. The Influence of the Call with a Mobile Phone on Heart Rate Variability Parameters in Healthy Volunteers. Industrial Health 46(4), 409–417.

Arora R, Gupta D, Chawla R, Sagar, Sharma A, Kumar R, Prasad J, Singh S, Samanta N, Kumar Sharma R.2005. Radioprotection by Plant Products: Present Status and Future Prospects. Phytother. Res 19, 1–22.

Basilico FC. 1999. Cardiovascular disease in athletes. Am J Sports Med 27(1), 108-121.

Braunwald, E, Bristow, MR. 2000. Congestive heart failure: fifty years of progress. Circulation 102, IV14-IV23

Bellieni C V, Acampa M, Maffei M, Maffei S, Perrone S, Pinto I, Stacchini N , Buonocore G. 2008. Electromagnetic fields produced by incubators influence heart rate variability in newborns. Arch Dis Child Fetal Neonatal Ed 93(4), F298-301.

Blettner M, Schlehofer B, Breckenkamp J. 2009. Mobile phone base stations and adverse health effects: phase 1 of a population-based, cross-sectional study in Germany. Occupational and Environmental Medicine 66(2), 118-123.

Chien KR. 1999. Stress pathways and heart failure. Cell. 98, 555-558.

Erogul O, Oztas E, Yildirim I, Kir T, Aydur E, Komesli G, Irkilata HC, Limak MK, Peker AF. 2006. Effects of electromagnetic radiation from cellular phone on human sperm motility: an in vitro study. Arc Med Res. 37, 840-3.

Frahm J, Lantow M, Lupke M, Weiss DG, Simko M. 2006. Alteration in cellular functions in mouse macrophages after exposure to 50 Hz magnetic fields. J Cell Biochem. 99, 168-177.

Gülçin I, Elias R, Gepdiremen A, Boyer L. 2006. Antioxidant activity of lignans from fringe tree (Chionanthus virginicus L.). Eur. Food Res. Technol. 223, 759–767.

Gülçin I, Elmastat MY, Aboul-Enein H.2007.Determination of Antioxidant and Radical Scavenging Activity of Basil (Ocimum basilicum L. Family Lamiaceae) Assayed by Different Methodologies”. Phytotherapy Research Phytother Res. 21, 354–361.

Hasani-Ranjbar SH, Larijani B, Abdollahi M. 2009. A Systematic Review of the Potential Herbal Sources of Future Effective Drugs in Oxidant-Related Diseases. Inflammation & Allergy – Drug Targets 8, 2-10.

Irmak M K, Fadillioglu E, Gule¸ M,Erdogan H, Yagmurca M, Akyol O. 2002. Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits. Cell Biochem. Funct. 20, 279-283

Jeong J H, Kim J S, Lee B C, Min YS, Kim DS, Ryu JS, Soh KS, Seo KM, Sohn UD. 2004. Influence of exposure to electromagnetic field on the cardiovascular system”.Autonomic & Autacoid, Pharmacology 25, 23-17.

Jauchem JR. 2007. Effects of low-level radio-frequency (3 kHz to 300GHz) energy on human cardiovascular, reproductive, immune, and other systems: A review of the recent literature .Int. J. Hyg. Environ. Health 211, 1–29.

Jauchem JR, Ryan KL, Frei MR, Dusch SJ, Lehnert HM, Kovatch RM. 2001. Repeated exposure of C3H/HeJ mice to ultra-wideband electromagnetic pulses: lack of effects on mammary tumors. Radiat Res 155, 369-377.

Javanmardi J, Stushno C, Locke E, Vivanco JM. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry 83, 547–550.

Kaya I, Yiğit N, Benli M. 2008. Antimicrobial Activity of Various Extracts of Ocimum Bsilicum and Observation of the inhibition effects on bacterial cells by use of scanning electron microscopy. Afr. J. Trad. CAM 5 (4), 363 – 369.

Khaki A, Heidari M, Ghaffari Novin M, Khaki AA. 2008. Adverse effects of Ciprofloxacin on testis apoptosis and sperm parameters in rats. Iran. J.Reprod. Med (6), 71-76.

Khaki A, Fathiazad F, Nouri M,Khaki AA. 2011. Effect of Ocimum basilicum on apoptosis in testis of rats after exposure to electromagnetic field. African Journal of Pharmacy and Pharmacology 5(12), 1534-1537.

Khaki A, Imani SAM, Golzar F. 2012. Effects of rosmarinic acid on male sex hormones (testosterone-FSH-LH) and testis tissue apoptosis after exposure to electromagnetic field (EMF) in rats. African Journal of Pharmacy and Pharmacology 6, 248 – 252,

Kruma Z, Andjelkovic M, Verhe R, Kreicbergs V. 2008. PHENOLIC COMPOUNDS IN BASIL, OREGANO AND THYME. Coupure Links 653, B-9000.

Lefkowitz RJ, Rockman HA, Koch WJ. 2000. Catecholamines, cardiac beta-adrenergic receptors, and heart failure. Circulation 101, 1634-1637.

Luo W. 1994.Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of beta-agonist stimulation. Circ. Res. 75, 401-409.

Lis-Balchin M, Buchbauer G, Ribisch K, Wenger MT. 1998.Comparative antibacterial effects of novel Pelargonium essential oils and solvent extracts. Lett. Appl. Microbiol 27, 135-141.

Marks AR. 2002. Ryanodine receptors, FKBP12, and heart failure. Front. Biosci 7, 970-977.

Meral I, Mert H, Nihat M, Deger Y, Yorukc I, Yetkind A, Keskine S. 2007. Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs. BRAIN RESEARCH 1169, 120– 124.

Mezei G, Cher D, Kelsh M, Edinboro CH, Chapman P, Kavet R . 2005. Occupational Magnetic Field Exposure, Cardiovascular Disease Mortality and Potential Confounding by Smoking. AEP 15(8), 622–629.

Niwano Y, Saito K, Yoshizaki F, Kohno M, Ozawa T. 2011. Extensive screening for herbal extracts with potent antioxidant properties”. J. Clin. Biochem. Nutr 48, 1 – 83.

Regula KM,Kirshenbaum LA. 2005. Apoptosis of ventricular myocytes: a means to an end. J Mol Cell Cardiol. 38, 3–13.

Supawan  B,  Chanida  P,  Nijsiri  R.  2007. Chemical compositions and antioxidative activities of essential oils from four Ocimum species endemic to Thailand. J .Health Res. 21(3), 201-6.

Roshangar B, Soleimani-Rad J, Ansaree R, Roshangar L. 2012. Effect of low frequency Electromagnetic Field on cardiovascular system: An ultrastructural and immunohistochemical study.Annals of Biological Research 3 (1), 81-87.

Sgherri C, Pinzino C, Navari-Izzo F, Izzo R. 2011. Contribution ofmajor lipophilic antioxidants to the antioxidant activity of basil extracts: an EPR study. J Sci Food Agric. 91, 1128–1134.

Sharifian A. 2009. Effect of extremely low frequency magnetic field on antioxidant activity in plasma and red blood cells in spot welders Int Arch Occup Environ Health 82(2), 259-66

Sharma M, Kishore K, Gupta S.K, Joshi S, S. Arya DH. 2001. Cardio protective potential of Ocimum sanctum in isoproterenol induced myocardial infarction in rats. Molecular and Cellular Biochemistry 225, 75–83

Tada H, Murakami Y, Shimomura K, Ishimaru K. 1996. Rosmarinic acid and related phenolics in hairy root cultures of Ocimum basilicum. Phytochemistry 42, 431-434.

Taimor G, Hofstaetter B, Piper HM. 2000. Apoptosis induction by nitric oxide in adult cardiomyocytes via cGMP-signaling and its impairment after simulated ischemia. Cardiovasc Res. 45, 588–94.

Wencker D, Chandra M, Nguyen K, Miao W, Garantziotis S, Factor SM, Shirani J, Armstrong RC, Kitsis RN. 2003. A mechanistic role for cardiac myocyte apoptosis in heart failure. J Clin Invest 111, 1497 –1504

Yoshikawa T, Naito Y, Kishi A. 1993. Role of active oxygen, lipid peroxidation, and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats. Gut 34(6), 732– 737.