Anatomical and genetical variability of brinjal (Solanum melongena L.) varieties based on RAPD marker

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Research Paper 30/09/2021
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Anatomical and genetical variability of brinjal (Solanum melongena L.) varieties based on RAPD marker

S.N Sima, Irin Lithy
Int. J. Biosci.19( 3), 157-165, September 2021.
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Abstract

The present investigation was aimed at studying the anatomical characters, quantitative measurement and anatomical features of the stem and leaf of five local varieties Golmakra, Debjhuri hajari, Golapi F1, Kanta begun and China of Solanum melongena of Solanaceae family. Genetic variation was assessed by RAPD DNA fingerprinting method with different primers. The anatomical investigation was conducted by cutting transverse slices from the materials, staining them using double-staining methods, and observing the findings using a powerful compound microscope. Four primers and RAPD molecular markers were used for the molecular analysis. The epidermis was documented as a single layer, according to anatomical study. In the stem, bi-collateral vascular bundles were visible and typically aligned in radial symmetry. RAPD based dendrogram were generated by the UPGMA method and cluster analysis revealed that distinct clusters were found. The preliminary results indicated both genetical and anatomical relationship of stem and leaf of brinjal varieties could be potential sources of germplasm for their use in variety improvement.

VIEWS 23

Ali Z, Xu ZL, Zhang DY, He XL, Bahadur S, Yi JX. 2011. Molecular diversity analysis of eggplant (Solanum  melongena L.) genetic resources. Genetics and Molecular Research 10(2), 1141-1155.

Afzal MA, Haque MM, Shanmugasundaram S. 2004. Random  Amplified Polymorphic DNA (RAPD) analysis of selected mungbean (Vigna radiata L. Wilczek cultivars.  Asian Journal of Plant Sciences 3(1), 20-24.

Barrell PJ, Meiyalaghan S, Jac.obs JME, Conner AJ.  2013. Applications of biotechnology and genomics in potato  improvement. Plant Biotechnology Journal 11, 907-920.

BBS. 2012. Statistics Year Book of Bangladesh, Bangladesh Bureau of Statistics, Ministry of Planning, Government of the people’s Republic of  Bangladesh, Dhaka.

Collonnier C, Fock I, Mariska I, Servaes A, Vedel F, Siljak-Yakovlev S, Souvannavong V. Sihachakr D. 2003. GISH confirmation of somatic hybrids between Solanum melongena  L. and S. torvum: assessment of resistance to  both fungal and bacterial wilts. Plan Physiol. Biochem 41(5), 459-470.

Cipriani G, Di Bella R, Testolin R. 1996. Screening RAPD primers for molecular taxonomy and cultivar fingerprinting I the genus Actinidia. Euphytica 90(2), 169-174.

Gleddie S, Keller W, Setterfield G. 1986. Eggplant.In: Evans, D.A.,Sharp W.R. (Eds.), Handbook of Plant Cell Culture, Techniques for Propagation and Breeding. MacMillan, New York, 3, 500-511 p.

Harish DK, Agasimani AK, Imamsaheb SJ, Patil Satish S.  2011. Growth and yield parameters in brinjal as influenced by organic nutrient management and plant protection conditions. Agricultural Science Research Journal 2(2), 221-225.

Hinata H. 1986. Eggplant (Solanum melongena L.).In: Bajaj, Y.P.S. (Ed.), Biotechnology in Agriculture and Forestry, Crop I. Springer, Berlin, 2, 363-370 p.

Jones N, Ougham H, Thomas H. 1997. Genome Mapping,  molecular markers and Marker assisted selection in crop  plants. New Phytologist 137, 165-177.

Khan MA, Rather TH, Dar MA, Kudesia R. 2013. Genetic diversity analysis of brinjal (Solanum melongena L.) accessions using RAPD markers. International Journal of Current Research 5(5), 1223-1223.

Kumar G, Meena BL, Kar R, Tiwari 2008. Morphological diversity inbbrinjal (Solanum  melongena L.) germplasm  accessions. Plant Genetic Resources 6(3), 232-236.

Rafalski JA, Tingey SV. 1993. Genetic diagnostics in plant  breeding: RAPDs, microsatellites and machines. Trends  Genet  9, 275-279.

Rashid M. 1993. Sabjee Bighan. Bangla   Academy, Dhaka 137-14 p.

Spooner DM, Knapp S. 2013. Solanum stipuloideum Rusby, the correct name for Solanum circaeifolium Bitter. American Journal of Potato Research 90(4), 301305.

Sing A, Borthakur A, Deka PC. 2001. Biotechnology of horticultural crops.2nd Eds. Vol.1.Naya Prakash.206 Bidhan Sarani, Kolkata, India p 35‐39.

Sarathbabu B, Varaprasad KS, Charaabarty SK, Sivaraj N.  1999. Status of resistant germplasm in eggplant (Solanum melongena L.) and wild Solanum species. Indian Journal of Plant Genetic Resources 12, 56-64.

Shaha MG. 1989. Combining ability estiamates and hybrid vigour in eggplant (Solanum melongena L.).M.Sc.(Ag) Thesis,  Department of Horticulture, BAU, Mymensingh.

Sihachakr D, Daunay MC, Serraf I, Chaput MH, Mussio I, Haricourt R, Rotino L, Ducreux G. 1994. Somatic  hybridization of eggplant (Solanum  melongena  L.) with its  close and wild relatives. In: Bajaj YPS (ed.) Biotechnology in Agriculture and Forestry: Somatic Hybridization in Crop Improvement, Springer, Berlin, 255-278 p.

Sneath PHA, Sokal RR. 1973. Numerical taxonomy: the principles and practice of numerical classification. San Francisco: Freeman. 573 p.

Thompson HC. 1951. Vegetable crops, McGraw Hill Book.Co.  New York. 368-370 p.

Thompson HC, Kelly WC. 1957. Vegetable crops, McGraw Hill Book. Co. New York. 500-505 p.

Tosti N, Negri V. 2002. Effiency of three PCR-based markers in assessing genetic variation among cowpea (vigna ungiculata spp unguiculata) landraces. Genome 45, 268-275.

Williams JGK, Hanafey MK, Rafalski JA, Tingey SV. 1990. Genetic analysis using random amplified polymorphic DNA markers.Methods lEnzymology 1218, 704-740.