Antagonistic activities of Trichoderma strains against chili anthracnose pathogen
Paper Details
Antagonistic activities of Trichoderma strains against chili anthracnose pathogen
Abstract
Antagonist activities of different Trichoderma strains were evaluated in vitro against Colletotrichum capsici, a causal agent of anthracnose fruit rot of chili. Dual culture test showed that Trichoderma strains effectively inhibited mycelia growth of the pathogen. T. harzianum IMI-392433 showed the highest inhibition (81.96 %) and mycelial overgrowth (78.98%). Also, metabolites having 80% concentration extracted from 30-day-old T. harzianum IMI-392433 revealed the highest PIRG (percentage inhibition of radial growth) value of 85.16 and 87.18% by using normal poison and modified bilayer poison agar technique, respectively. Further, metabolites extracted from 30-day-old T. harzianum IMI-392433 at a concentration of 2000 mgL-1 completely inhibited spore germination and germ tube elongation of the pathogen. Also, severity of anthracnose was significantly decreased as compared with the control (2% methanol) when chili fruits were soaked in 2000 mgL-1 of 30-day-old metabolites from T. harzianum strains. Importantly, metabolites extracted from T. virens IMI-392430 (71.09) and T. pseudokoningii IMI-392431 (69.52) were found to be most efficient in the inhibition of disease severity. Taken together, our data suggest that Trichoderma strains especially T. harzianum IMI-392433 is a potential antagonistic organism and can be used to control the anthracnose disease caused by C. capsici.
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Mohammad Ahsanur Rahman, Mohammad Anowar Razvy, Mohammad Firoz Alam (2013), Antagonistic activities of Trichoderma strains against chili anthracnose pathogen; IJMM, V1, N1, September, P7-22
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