Biochemical and cultural characterization of Xanthomonas axonopodis pv. citri isolated from infected citrus plants from Bahawalpur, Pakistan
Paper Details
Biochemical and cultural characterization of Xanthomonas axonopodis pv. citri isolated from infected citrus plants from Bahawalpur, Pakistan
Abstract
Citrus is an important fruit grown all over the world, but it is vulnerable to attack of Xanthomonas axonopodis pv. citri (Xac), causative agent of citrus canker. It is documented as most devastating disease leading to great economic losses by affecting overall fruit production in citrus. In contemporary study Xac was isolated from the infected plant samples which were collected from Bahawalpur by using nutrient agar medium. The bacterium was purified on nutrient agar medium by streaking method. Confirmation of Xac was done by its morphological, biochemical and cultural features. It showed positive result in KOH solubility test, starch hydrolysis test, casein hydrolysis, catalase test, and citrate utilization test while it expressed negative result in fluorescent pigmentation, Staining test, indole production and Kovac’s oxidase test. The bacterium colonies appeared as mucoid, convex, yellow to orange color on NA medium; flattened or slightly raised, bright to pale yellow color, mucoid colonies on YDCA medium. This study is helpful for researchers and scientist for accurate identification of Xac which is necessary for in-vitro evaluation of different chemicals to find out most affective chemical against canker disease in field.
Al-Snafi AE. 2016. Nutritional value and pharmacological importance of citrus species grown in Iraq. IOSR Journal of Pharmacy 6(8), 76-108.
Aneja K. 2003. Experiments in microbiology, plant pathology and biotechnology. New Age International, New Delhi.
Bhure SS, Bramhankar SB, Thakur KD, Wasnik DG, Pawar RD, Labhasetwar AA, Kakad SA, Ravali T, Sarode CA. 2019. Physiological and biochemical characterization of Xanthomonas axonopodis pv. citri: A gram negative bacterium causing citrus canker. International Journal of Chemical Studies 7(1), 1941-1944.
Cappuccino JG, Sherman N. 2008. Microbiology: A Laboratory Manual. Pearson Benjamin Cummings, San Francisco, CA, USA.
Cowan S, Steel K. 1974. Manual for identification for medical bacteria. 2nd Cambrige Uni. Press, England.
Ference CM, Gochez AM, Behlau F, Wang N, Graham JH, Jones JB. 2018. Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management. Molecular plant pathology 19(6), 1302-1318.
Garibaldi J. 1967. Media for the enhancement of fluorescent pigment production by Pseudomonas species. Journal of bacteriology 94(5), 1296-1299.
Gottig N, Garavaglia B, Garofalo C, Zimaro T, Sgro G, Ficarra F, Gehring C. 2010. Mechanisms of infection used by Xanthomonas axonopodis pv. citri in citrus canker disease. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology 1(13), 196-204.
Gottwald TR, Graham JH, Schubert TS. 2002. Citrus canker: the pathogen and its impact. Plant Health Progress 3(1), 15.
Graham JH, Gottwald TR, Cubero J, Achor DS. 2004. Xanthomonas axonopodis pv. citri: factors affecting successful eradication of citrus canker. Molecular plant pathology 5(1), 1-15.
Hall GS. 2013. Bailey & Scott’s Diagnostic Microbiology, 13th Edn. In: American Society for Clinical Pathology.
Isenberg HD, Sundheim LH. 1958. Indole reactions in bacteria. Journal of bacteriology 75(6), 682.
Jabeen R, Iftikhar T, Batool H. 2012. Isolation, characterization, preservation and pathogenicity test of Xanthomonas oryzae pv. oryzae causing BLB disease in rice. Pak. J. Bot 44(1), 261-265.
Jabeen T, Arshad HMI, Saleem K, Ali S, Ullah E, Naureen S, Babar MM. 2016. Morphological and biochemical characterization of Xanthomonas axonopodis pv. citri isolates causing citrus canker disease in Pakistan. PSM Microbiology 1(1), 10-17.
King EO, Ward MK, Raney DE. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. The Journal of laboratory and clinical medicine 44(2), 301-307.
Kovacs N. 1956. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178(4535), 703.
Li J, Wang N. 2011. Genome-wide mutagenesis of Xanthomonas axonopodis pv. citri reveals novel genetic determinants and regulation mechanisms of biofilm formation. PLoS One 6(7), e21804.
Mahmood R, Rehman A, Ahmad M. 2014. Prospects of biological control of citrus insect pests in Pakistan. Journal of Agricultural Research 52(2).
Mendonça L, Zambolim L, Badel J. 2017. Bacterial citrus diseases: major threats and recent progress. Journal of Bacteriology & Mycology 5(4).
Mohammadi M, Mirzaee M, Rahimian H. 2001. Physiological and biochemical characteristics of Iranian strains of Xanthomonas axonopodis pv. citri, the causal agent of citrus bacterial canker disease. Journal of Phytopathology 149(2), 65-75. https://doi.org/10.1046/j.1439-0434.2001.00570.x
Muthukumar A, Udhayakumar R, Naveenkumar R. 2017. Role of bacterial endophytes in plant disease control. In Endophytes: Crop Productivity and Protection (pp. 133-161).
Naseer MAR, Mubashir M, Muhammad A, Sarfraz H, Abid M. 2019. Effect of marketing channel choice on the profitability of citrus farmers: evidence form Punjab-Pakistan. Pakistan Journal of Agricultural Sciences 56(4), 1003-1011.
Niphadkar NP, Burks TF, Qin J, Ritenour MA. 2013. Estimation of citrus canker lesion size using hyperspectral reflectance imaging. International Journal of Agricultural and Biological Engineering 6(3), 41-51.
Patil A, Ambadkar C, Kanase K, Kashid V. 2017. Cultural and Morphological Characteristics of Different Xanthomonas axonopodis pv. punicae Isolates on Nutrient Agar Media. International Journal of Current Microbiology and Applied Sciences 6(11), 1678-1683.
Prakash M, Karmegam N. 2012. In-vitro antibacterial activity of certain plant extracts against plant disease causing bacteria isolated from citrus plant. International Journal of Current Microbiology and Applied Sciences 1(1), 1-11.
Ryu E. 1940. A Simple Method of Differentiation Between Gram-positive and Gram-negative Organisms Without Staining. Kitasato Archives of Experimental Medicine 17, 58-63.
Salle A. 1961. Fermentation of carbohydrates and related compounds. Laboratory manual on Fundamental Principles of Bacteriology. 5th ed. McGraw Hill Book Company, Inc. New York, USA 94-98.
Schaad N, Postnikova E, Lacy G, Sechler A, Agarkova I, Stromberg P, Vidaver A. 2005. Reclassification of Xanthomonas species pathogenic on citrus. Systematic and Applied Microbiology 28, 494-518.
Schaad NW, Jones JB, Chun W. 2001. Laboratory guide for the identification of plant pathogenic bacteria. American Phytopathological Society (APS Press).
Schaad N, Stall R. 1988. Xanthomonas. Laboratory guide for identification of plant pathogenic bacteria 2, 81-94.
Schubert TS, Rizvi SA, Sun X, Gottwald TR, Graham JH, Dixon WN. 2001. Meeting the challenge of eradicating citrus canker in Florida again. Plant Disease 85(4), 340-356.
Simione F, Brown E, Buck C. 1991. ATCC preservation methods. American Type Culture Collection.
Sujatha B, Sai Gopal D. 2010. Isolation and characterization of Xanthomonas axonopodis from citrus aurantifolia christm (Swingle). The Bioscan 5(3), 373-376.
Suslow T, Schroth M, Isaka M. 1982. Application of a rapid method for Gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology (USA).
Ullah MI, Riaz M, Arshad M, Khan AH, Afzal M, Khalid S, Mehmood N, Ali S, Khan AM, Zahid SMA, Riaz M. 2019. Application of Organic Fertilizers Affect the Citrus Leafminer, Phyllocnistis citrella (Lepidoptera: Gracillariidae) Infestation and Citrus Canker Disease in Nursery Plantations. International journal of insect science 11, 1179543319858634
Verma J, Singh R. 1976. Races of Xanthomonas malvacearum, loss in their virulence and the protective effect of avirulent strains, heat-killed cells and phylloplane bacteria. Journal of Plant Diseases and Protection 83(12), 748-757.
Muhammad Arslan Mahmood, Muhammad Naveed Aslam, Muhammad Atiq, Nasir Ahmed Rajput, Muhammad Usman, Arslan Sharif, Kiran Fatima, Muhammad Zeeshan Talib, Naveed Aslam, Hamza Tariq (2020), Biochemical and cultural characterization of Xanthomonas axonopodis pv. citri isolated from infected citrus plants from Bahawalpur, Pakistan; JBES, V16, N3, March, P70-79
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