home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Chromium-tolerant bacteria in diversified soil microbial community in the bank of tannery waste water discharging canal of East Calcutta, West Bengal

Paper Details

Research Paper 01/03/2014
Views (324) Download (14)
current_issue_feature_image
publication_file

Chromium-tolerant bacteria in diversified soil microbial community in the bank of tannery waste water discharging canal of East Calcutta, West Bengal

Piku Sen, Anandita Pal, Budhhadeb Chattopadhyay, D. Pal
J. Bio. Env. Sci.4( 3), 233-238, March 2014.
Certificate: JBES 2014 [Generate Certificate]
Key: BioremediationChromate-reducing microbial strainChromium (6+)Cr resistant bacteriaMicrobial community

Abstract

Studies have revealed that East Calcutta Wetlands harbor a variety of microbial population possessing diverse genetic characters with versatile enzymatic and metabolic activities. They carry out bioremediation of different toxins, pollutants, heavy metals etc. Due to the discharge of improperly treated effluents from tanneries and other industries in and around Calcutta City, carcinogenic chromium (Cr6+) contamination of both surface water and ground water has been reported in the East Calcutta Wetland area. A few examples in microbial diversity in this area include Rhodococcus sp., Bacillus sp., Pseudomonus sp., Azotobacter sp., Aeromonas sp. etc. The primary objective of this study was to isolate and identify a potent chromate-reducing bacterial strain. Cr6+ analysis was done and the bacterial population was enumerated by analyzing soil samples from different locations. The majority of the chromate-resistant bacteria isolates from the tannery effluents enriched soil showed a minimum inhibitory concentration (MIC) of Cr6+ ranging from 50 to 750 mg l-1. About 39.47% of the total 38 isolates of bacterial strains were able to grow at 200 mg l– 1 Cr6+. The potent Cr6+-resistant isolates showed a very high tolerance level to 750 mg l-1 and were able to show 100% Cr6+ reduction up to 200 mg l-1 within 48 h. The present study conclusively demonstrates the ability of native microbial population present in tannery effluent to reduce Cr6+ compounds. Furthermore, all the isolates have shown great potential for bioremediation of Cr6+-containing wastes. It is also reported that plant roots release some inorganic and organic compounds which aid the microbial community in the bioremediation of heavy metal pollutants in the soil. This approach permits the selection of bacterial strains which could be used for specific environmental cleanup operations.

VIEWS 17
Cover PDF

Katz SA, Salem H. (1993). “The toxicology of chromium with respect to its chemical speciation: a review.” Journal of Applied Toxicology 13(3), 217-24.

Carventes C, Campose Gracia J, Devars S, Guierrez-Corona F, Loza-Tavera H, Torres-Guzman JC, Moreno-Sanchez R. 2001. Interactions of chromium with microorganisms and plants. FEMS Microbiology Reviews 25, 335-347.

Camargo FAO, Bento FM, Okeke BC, Frankenberger WT. (2003)a. Chromate reduction by chromium –resistant bacteria isolated from soils contaminated with dichromate. Journal of Environmental Quality 32, 1228-1233.

Kamaludeen SP, Arunkumar KR, Avudainayagam S, Ramasamy K. 2003. Bioremediation of chromium contaminated environments., Indian of Journal Experimental Biology 41, 972-985.

Losi ME, Amrein C, Frankenberger WT. 1994a. Environmental biochemistry of chromium, Reviews of Environmental Contamination and Toxicology 36, 91-121.

Alvarez  AH, Moreno-Sanchez R, Cervantes C. 1999. Chromate efflux by means of ChrA chromate resistance protein from Pseudomonas aeruginosa. Journal of Bacteriology 181, 7398-7400.

Pattanapipitpaisal P, Brown NL, Macaskie LE. 2001. Chromate reduction and 16SrRNA identification of bacteria isolated from a Cr(VI)-contaminated site, Applied Microbiology and Biotechnology 57, 257-261.

Wang P, Mori T. 1990. Membrane –associated chromate reductase activity from Enterobacter cloacae, Journal of Bacteriology 172, 1670-1672.

Clark DP. 1994. Chromate reductase activity of Enterobactor aero- genes is induced by nitrite. FEMS Microbiology Letter 122, 233-237.

Shen H, Wang YT. 1994. Biological reduction of chromium by E. coli. Journal of Environmental Engineering 120, 560-560.

Campos J, Martinez-Pacheco M, Cervantes  C. 1995. Hexavalent Chromium reduction by a chromate resistant Bacillus sp. Strain, Antonie Von . Leeuwenhoek 68, 203-208.

Basu M, Bhattacharyya S, Paul AK. 1997, Isolation and characterization of chromium resistant bacteria from tannery effluent. Bulletin of Environmental Contamination and Toxicology 58, 535-542.

APHA. 1995. Standars methods for the examination of water and waste water , nineteenth ed. American Public Health Association, Washington, DC.

Isaac RA, Kerber JD. 1971. Atomic absorption Biochemistry and flame photometry: techniques and uses in soils, plant, and water analysis. In Instrumental Methods for Analysis of Soils and Plant Tissue (ed. L.M. Walsh), 17-37 p. Soil Science Society of America, Madison, Wisconsin.

Mergeay M. 1995. Heavy metal resistances in microbial ecosystems . In: Akkermans ADL, van Elsas JD, de Bruijin FJ(eds) Molecular microbial ecology manual, Dordrecht: Kluwer Academic Publishers, p6.1.7/1- 6.1.7/17.

Katiyar SK, Katiyar R. (1997). Microbes in control of heavy metal pollution. Advance Microbiology Biotechnology 19, 330-344.

Carvantes C, Campos-Garcia J, Devars S, Gutierrez-Corona F, Loza-Tavera H, Torres-Guzman JC, Moreno-Sanchez R. 2001. Interactions of chromium with microorganisms and plants. FEMS Microbiology Review 25, 335–347

Camargo FAO, Bento FM, Okeke BC, Frankenberger WT. 2003a. Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate. Journal of Environmental Quality 32, 1228–1233.

Kamaludeen SP, Arunkumar KR, Avudainayagam S, Ramasamy K. 2003. Bioremediation of chromium contaminated environments, Indian Journal of Experimental Biology 41, 972–985.

Quintelas C, Sousa E, Silva F. 2006, Competitive biosorption of ortho-cresol, phenol, chlorophenol and chromium (VI) from aqueous solution by a bacterial biofilm supported on granular activated carbon, Process Biochemistry 41, 2087–2091.

Sierra-Alvarez R.  2007.  Fungal  bioleaching  of metals in preservative-treated wood,Process Biochemistry 42, 798–804.

Oliveira EA, Montanher SF, Andrade AD. 2005. Equilibrium studies for the sorption of chromium and nickel from aqueous solutions using raw rice bran, Process Biochemistry40, 3485–3490.

Ramırez-Ramırez R, Calvo-Mendez C, Avila-Rodrıguez M. 2004. Cr (VI) reduction in a chromate-resistant strain of Candida maltosa isolated from the leather industry, Antonie Von . Leeuwenhoek 85, 63–68.

Submit Manuscript