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Diagnosis and management of diabetic nephropathy using novel biomarkers, CXCL16 and TNF-α receptors 1and 2

Research Paper | October 1, 2019

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Fahmy Tawfik Ali, Eman M. Abd El Azeem, Samia Abdelsalam Hassan, Hala Fawzy Hekal

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Int. J. Biosci.15( 4), 515-523, October 2019

DOI: http://dx.doi.org/10.12692/ijb/15.4.515-523


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CXCL16 is expressed at a low level in epithelial cells in the normal kidney and play a crucial role in regulating inflammation and tissue injury. The function of TNFα is mediated by two structurally distinct receptors, TNFR1 and TNFR2. The reliability of current tests in predicting the onset, progression and response to various regimens for diabetic nephropathy is still under debate; and it has engendered a search for more sensitive and specific biomarkers. This study was aimed to evaluate the role of some relevant biomarkers in the pathogenesis of DN and that potentially may be used to predict the onset and/or monitor the progression of nephropathy. A total of Seventy Egyptian subjects, including 28 patients with diabetic nephropathy (DN group), 20 type 2 diabetes mellitus patients (T2DM group), and 22 healthy controls of similar age and gender (control group), were enrolled in this study. Serum levels of CXCL16 and TNFR1 & TNFR2 were measured using ELISA technique. Diabetic and renal biomarkers were measured in all subjects. The results showed drastic elevation in the levels of CXCL16, TNFR1, TNFR2, kidney function tests (except eGFR was highly significant decreased) and lipid profile parameters in diabetic nephropathy patients when compared to the control group. CXCL16, TNFR1 and TNFR2 showed high accuracy in the nephropathy group. TNFRs 1 & 2 and CXCl16 may be early, highly sensitive and specific markers and they could be used as a useful promising biomarker for early detection of diabetic nephropathy.


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