Genetic diversity and phylogeography of hawksbill turtle in the Persian Gulf

Paper Details

Research Paper 01/04/2014
Views (309) Download (12)
current_issue_feature_image
publication_file

Genetic diversity and phylogeography of hawksbill turtle in the Persian Gulf

M. Tabib, F. Frootan, M. Askari Hesni
J. Bio. Env. Sci.4( 4), 51-57, April 2014.
Certificate: JBES 2014 [Generate Certificate]

Abstract

Genetic diversity and phylogeography of Hawksbill sea turtle was studied by using sequencing of the mitochondrial DNA (D-loop and tRNA pro region). 45 dead embryos were collected from the Qeshm (25 samples) and Kish Islands (20 samples) in the Persian Gulf. Analysis of sequence diversity showed over 890 bp of the mtDNA D-loop and tRNA pro region revealed 5 polymorphism sites and 7 haplotypes. Two new haplotypes were submitted on NCBI gene bank as Iran3 with GU997696 accession number and Iran7 with JN627023 accession number. Haplotype (h) and nucleotide (π) diversity was (h= 0.313 and π= 0.0006) for Qeshm Island and (h= 0.111 and π=0.0002) for Kish Island, respectively. Total haplotype diversity was calculated as 0.212, which had demonstrated low genetic diversity in this area. The Fst and P-value showed that Hawksbill turtles of Qeshm and Kish Islands are different populations. A comparison of our data with previous studies showed a relationship between the Persian Gulf and Indo-Pacific Hawksbill turtles and also sharp relationship with east Atlantic. Hawksbill turtles of the Persian Gulf have been migrated from the Pacific and the Oman Sea into this area. As a result, evidence of populations migration was not found from the West.

VIEWS 16

Abreu-Grobois A, Horrocks J, Formia A, Leroux R, Velez-Zuazo X, Dutton PH, Soares L, Meylan A, Browne D. 2006. New mtDNA dloop primers which work for a variety of marine turtle species may increase the resolution capacity of mixed stock analysis. In: Frick MG, Panagopoulou A, Rees A, Williams KL (eds) 26th Annual Symposium on Sea Turtle Biology and Conservation, Crete, Greece, p. 179.

Bass AL. 1996. Application of isolation by distance models to hawksbill turtle (Eretmochelys imbricata) nesting sites in the caribbean. In: Proceedings of the International Symposium on Sea Turtle Conservation Genetics (Bowen BW and Witzell WN, eds.). NOAA Technical Memorandum. National Marine Fisheries Service, Southeast Fisheries Science Center, 12-14 September 1995, Miami.

Bjorndal KA. 1997. Foraging Ecology and Nutrition of Sea turtles. In Lutz, P.L.: Musick, J.L. (eds) The Biology of Sea turtles vol 1. CRC Press, Boca Raton, FL, p.199−231.

Blumenthal JM, Austin TJ, Bothwell JB, Broderick AC, Ebanks-Petrie G, Olynik JR, Orr MF, Solomon JL, Witt MJ, Godley BJ. 2009. Diving Behavior and Movements of Juvenile Hawksbill turtles ( Eretmochelys imbricata) on a Caribbean coral reef. Journal of Coral Reef Studies 28: 55–65.

Bolten AB, Bjorndal KA, Martins HR, Dellinger T, Biscoito MJ, Encalada SE, Bowen BW. 1998. Transatlantic developmental migrations of loggerhead sea turtles demonstrated by mtDNA sequence analyses. Journal of Ecological Applications. 8: 1–7.

Bowen BW, Bass AL, Garcia-Rodriguez A, Diez CE, Van Dam R, Bolten A, Bjorndal KA, Miyamoto MM, Ferl RJ. 1996. Origin of hawksbill turtles in a Caribbean feeding area as indicated by genetic markers. Journal of Ecological Applications. 6: 566-572.

Bowen BW, Bass AL, Soares L, Toonen RJ. 2005. Conservation implications of complex population structure: lessons from the loggerhead turtle (Caretta caretta). Journal of Molecular Ecology. 14: 2389–2402.

Bowen BW, Karl SA. 2007. Population genetics and phylogeography of sea turtles. Journal of Molecular Ecology. 16: 4886-4907.

Broderick D, Moritz C, Miller JD, Guine M. 1994. Genetic studies of the Hawksbill turtle (Eretmochelys imbricate): evidence for multiple stocks in Australian waters. Pacific Conservation Biology. 1: 123-131.

Broderick D, Moritz C. 1996. Hawksbill Breeding and Foraging Populations in the Indo-Pacific Region. In: Proceedings of the International Symposium on Sea Turtle Conservation Genetics (Bowen BW and Witzell WN, eds.). NOAA Technical Memorandum. National Marine Fisheries Service, Southeast Fisheries Science Center, 12-14 September 1995, Miami.

Carr AF. 1967. So Excellent a Fish: A Natural History of Sea Turtles. Scribner, New York.

Fitzsimmons NN, Limpus CJ, Norman JA, Goldizen AR, Miller JD, Moritz C. 1997a. Philopatry of male marine turtles inferred from mitochondrial DNA markers. Proceedings of the National Academy of Sciences, USA, 94: 8912–8917.

Fitzsimmons NN, Moritz C, Limpus CJ, Pope L, Prince R. 1997b. Geographic structure of mitochondrial and nuclear gene polymorphisms in Australian green turtle populations and male-biased gene flow. Journal of Genetics 147: 1843–1854.

Groombridge B, Luxmoore R. 1989. The Green Turtle and Hawksbill (Reptilia: Cheloniidae): World Status, Exploitation, and Trade. CITES Secretatiat, Lausanne.

Hendrickson JR. 1958. The green sea turtle, Chelonia mydas (Linn.) in Malaya and Sarawak. Proceedings of the Zoological Society of London, 130: 455–535.

Hillis DM, Moritz C. 1990. Molecular Taxonomy. Sinauer Associates Inc. Publishers, Sanderland.

IUCN (International Union for Conservation of Nature). 2010. The IUCN red list of threatened species, Version 2010.1. Available:http: //www. iucnredlist.org.

Kazemi Nezhad SR, Modheji E, Zolgharnein H. 2012. Polymorphism analysis of Mitochondrial Dna control region of Hawksbill turtles (Eretmochelys imbricata) in the Persian Gulf. Journal of Fisheries and Aquatic Science. 7(5): 339- 345.

Kinunen W, Walczak PS. 1971. Persian Gulf Sea Turtle Nesting Surveys. Job Completion Rept., Division of Research Development, F7-F50.

Koike H, Okayama T, Baba Y, Diaz R. 1996. Conservation Genetics for the CITES-Listed Animals – Mitochondrial DNA Analysis Using the Scutes of Hawksbill Turtles. International Symposium on Network and Evolution of Molecular Information, 20-22 April 1996, Tokyo.

Lara-Ruiz P, Lopez GG, Santos FR, Soares LS. 2006. Extensive hybridization in hawksbill turtles (Eretmochelys imbricata) nesting in Brazil revealed by mtDNA analyses. Conservation Genetics. 7: 773-781.

Maffucci  F,  Kooistra  WHCF,  Bentivegna  F. 2006. Natal origin of loggerhead turtles, Caretta caretta, in the neritic habitat off the Italian coasts, Central Mediterranean. Biological Conservation. 127: 183-189.

Mobaraki A. 2004a. Nesting of the hawksbill turtle in Shidvar Island, Hormozgan Province, Iran. Mar. Marine Turtle Newsletter. 103: p. 13.

Mohd Arshaad W, Syed Abdul Kadir SA. 2010. Identification of the stock population of green and hawksbill turtles in the southeast asian region. (unpublished).

Monozon- Argello C, Rico C, Marco A, Lopez P, Lopez- Jurado LF. 2010. Genetic characterization of eastern Atlantic hawksbill turtles at a foraging group indicates major undiscovered nesting populations in the region. Journal of Experimental Marine Biology and Ecology . 387: 9-14.

Nishizawa H, Okuyama J, Abe O, Kobayashi M, Arai N. 2012. Mitochondrial DNA Variation in Hawksbill Turtles (Eretmochelys imbricata) Nesting on Ishigaki Island, Japan. Marine Turtle Newsletter. 132: 1-2.

Okayama T, Diaz R, Koike H, Diez CE. 1996. Mitochondrial DNA analysis of the Hawksbill turtle. I. Haplotype detection among samples in the Pacific and Atlantic Oceans. International Symposium on Network and Evolution of Molecular Information, 20-22 April 1996, Tokyo.

Okayama T, Diaz-Fernandez R, Baba Y, Halim M. 1999. Genetic diversity of the hawksbill turtle in the Indo- Pacific and Caribbean regions. Chelonian Conservation and Biology. 3: 362-367.

Owens DW, Grassman MA, Hendrickson JR. 1982. The imprinting hypothesis and sea turtle reproduction. Journal of Herpetologica, 38: 124–135.

Pritchard PCH, Mortimer JA. 1999. Taxonomy, external morphology, and species identification. In: K.L.Eckert etal, (eds). Research and management techniques for the conversation of sea turtles. IUCN/SSC Marine Turtle Specialist Group Publication 4: 21-38.

Saccone C, Giorgi CO, Gissi C, Pesole G, Reyes A. 1999. Evolutionary genomics in metazoa: the mitochondrial DNA as a model system. Journal of Genetics 238: 195-209.

Schneider S, Roessli D, Escoffier L. 2000. Arlequin: a software for population genetics data analysis, version 2.000. Genetic and Biometry Laboratory, Departement of Anthropology, University of Geneva, Switzerland.

Tabib M, Zolgharnein H, Mohammadi M, Salari-Aliabadi MA, Qasemi A, Roshani S, Rajabi Maham H, Frootan F. 2011. mtDNA variation of the critically endangered hawksbill turtle (Eretmochelys imbricata) nesting on Iranian islands of the Persian Gulf. Genetics and Molecular Research (3): 1499-1503. Available:http://www.funpecrp. com.br.

Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4. Molecular Biology and Evolution. 24: 1596-1599.

Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research. 22: 4673-4680.

Troëng S, Rankin E. 2005. Long-term conservation efforts contribute to positive green turtle Chelonia mydas nesting trends at Tortuguero, Costa Rica. Biological Conservation 121: 111–116.

Trujillo-Arias N, Amorocho D, Lopez DC, Mejia-Ladino LM. 2011. Caracterizacion genetica de la Tortuga carey (Eretmochelys imbricata) en Colombia, basada en la region control de and mt. CONGRESO: III Congreso Colombiano de Zoología LUGAR DE CELEBRACIÓN: Colombia. AÑO:. 21-26 novomber 2010. Colombia.

Van dam RP, Diez CE, Balazs GH, Colon LAC, McMillan WO, Schroeder B. 2008. Sex-specific migration patterns of hawksbill turtles breeding at Mona Island, Puerto Rico. Journal of Endangered Species Research. 4: 85–94.

Velez-Zuazo X, RamosWD, Van Dam RP, Diez CE, Serrano X, Abreu- Grobois A, McMillan WO. 2008. Dispersal, recruitment and migratory behavior in a hawksbill sea turtle population. Journal of Molecular Ecology 17: 839- 853.

Wolf PG, Soltis PS. 1991. Genetic divergence and relationships in the Ipomopsis aggregata complex and related species (Polemoniaceae). American Journal of Botany 72: 232-244.

Wright S. 1951. The genetical structure of populations. Annals of Eugenics Journal 15: 323-354.