Histological study of leaves symptoms of Brassica napus and Raphanus brassica infected by Alternaria brassicae
Paper Details
Histological study of leaves symptoms of Brassica napus and Raphanus brassica infected by Alternaria brassicae
Abstract
Alternaria leaf spot can be a devastating disease as the mechanism by which the fungus Alternaria brassicae (Berk.) Sacc. spread in canola is not yet fully understood. It is essential to understand the infection process and mechanisms of resistance by studying biotic and abiotic factors. In the present study, two abiotic elicitors-Salicylic acid (200 ppm), and Milor MZ (Mancozeb 64% + Metalaxyl 8% WP) fungicide (100 ppm) along with the biotic elicitor Pseudomonas fluorescens (PF83), were examined to induce systemic resistance in Brassica napus AACC (2n=38) and Raphanus brassica AARR (2n=38) against Alternaria leaf spot disease. The histological study revealed that R. brassica exhibited resistance to Alternaria leaf spot disease. Fungal colonies were observed on R. brassica leaves at 120 hours post-inoculation (hpi), and Similar observations were seen on B. napus leaves after 24 hpi. However, signs of the pathogen, including hyphae, conidia, appresoria, and dead cells, were present on the surface of the infected leaf in both genotypes but were not observed in plants treated with biotic and abiotic elicitors. Thus, the biotic elicitors PF83 and the abiotic elicitors SA and Milor MZ significantly reduced (P≤0.5) lesion sizes of pathogen at 24, 84, and 120 hpi in both genotypes. Finally, the results suggested that R. brassica produced resistance to Alternaria leaf spot. Additionally, the application of PF83 isolate is considered a useful tool for enhancing protection methods for canola genotypes.
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Jawadayn Talib Alkooranee (2025), Histological study of leaves symptoms of Brassica napus and Raphanus brassica infected by Alternaria brassicae; IJAAR, V26, N1, January, P8-14
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