International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Determination of phenolic compounds and evaluation of the antioxidant properties of four plants from Burkina Faso

By: Windmi Kagambega, Lassina Ouattara, Crépin I. Dibala, Judith N. Semporé, Mamoudou H. Dicko

Key Words: Phenolic compounds, Antioxidant activities, Daniella oliveri, Sclerocarya birrea, Maranthes polyandra, Ptelopsis suberosa.

Int. J. Biosci. 17(1), 95-105, July 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.1.95-105

Certification: ijb 2020 0006 [Generate Certificate]

Abstract

Daniella oliveri (Caesalpiniaceae), Sclerocarya birrea (Anacardiaceae), Maranthes polyandra (Chrysobalanaceae), Ptelopsis suberosa (Combretaceae) have long been regarded as the medicinal plants. This research work was designed to evaluate the polyphenolic content, and antioxidant activity of extracts and fractions of these four plants of Burkina Faso. The extraction of the bioactive compounds was carried out by hydroethanolic maceration followed by a liquid-liquid fractionation with solvents of increasing polarities. Total phenols and flavonoids were determined by the Folin-Ciocalteu reagent and aluminum trichloride spectrophotometer, respectively. The antioxidant activities of the samples were evaluated by various in vitro assays like total reducing power, DPPH scavenging and ABTS radical cation decolorization assays. The highest extraction yields were obtained with ethyl acetate and butanol fractions. Of these four plants, only the species Ptelopsis suberosa had the highest levels of phenolic compounds. The total phenol and total flavonoid contents were respectively 22.37 ± 0.21 mg EAG / 100 mg and 16.05 ± 0.13 mg EQ / 100 mg hydroethanolic extract, 21.31 ± 0.21 mg EAG / 100 mg and 11.82 ± 1.98 mg EQ / 100 mg ethyl acetate fraction and 18.07 ± 0.41 mg EAG / 100 mg and 11, 22 ± 1.31 mg EQ / 100 mg butanolic fraction. The low levels of phenolic compounds were obtained with the species Maranthes polyandra. The results of the antioxidant activities showed that the hydroethanolic extract of Ptelopsis suberosa and the fraction of ethyl acetate of Maranthes polyandra present the best antioxidant activities with respectively an IC50 of 0.36 ± 0.19 and 0.47 ± 0.12 μg/mL and an inhibitory power of the radical ABTS of 32.28 ± 0.68 and 34.81 mg /g. The present study demonstrated that these four plants are an important source of phenolic compounds and have interesting antioxidant properties.

| Views 68 |

| Views 68 |

Determination of phenolic compounds and evaluation of the antioxidant properties of four plants from Burkina Faso

Agbangnan DCP. 2011. Extraction et concentration d’extraits polyphénoliques naturels, bioactifs et fonctionnels par procédés membranaires: Caractérisation des structures moléculaires d’extraits du sorgho rouge (sorghum caudatum) du Bénin. Thèse de doctorat, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Bénin, p 355

Arvouet-Grand A, Vennat B, Pourrat A, Legret P. 1994. Standardisation d’un extrait de propolis et identification des principaux constituants. Journal de Pharmacie de Belgique 49(6), 462-468.

Autran JC. 2010. Le guide de phytothérapie créole : Se soigner par les plantes créoles. Ile Reunion, édition Orphie, p 371.

Bonnefoy M, Drai J, Kostka T. 2002. Les antioxydants pour retarder les effets du vieillissement, faits et perspectives: Peut-on prévenir le vieillissement ? Presse Medical 31(24), 1174-1184.

Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of à Free Radical Method to Evaluate Antioxidant Activity. Food Science and Technology-Lebensmittel-Wissenschaft & Technologie 28(1), 25-30.

https://doi.org/10.1016/S0023-6438(95)80008-5

Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M, Abdelly C. 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. C R Biologies 331, 372-379.

Hamza OJM, Van den Bout-van den Beukel Carolien JP, Matee Mecky IN, Moshi Mainen J, Mikx Frans HM, Selemani Haji O, Mbwambo Zakaria H, Van der Ven André JAM, Verweij Paul E. 2006. Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections. Journal of Ethnopharmacology 108, 124-132.

https://doi.org/10.1016/j.jep.2006.04.026

Huang D, Ou B, Prior B. 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry 53(6), 1841-1856

https://doi.org/10.1021/jf030723c

Iwueke AV, Nwodo OFC. 2008. Antihyperglycaemic effect of aqueous extract of Daniella oliveri and Sarcocephalus latifolius roots on key carbohydrate metabolic enzymes and glycogen in experimental diabetes. Biokemistri. 20, 63–70.

Khan MK. 2010. Polyphénols d’Agrumes (flavanones): extraction de glycosides de la peau d’orange, synthèse de métabolites chez l’homme (glucuronides) et étude physico-chimique de leur interaction avec le sérum albumine. Thèse de doctorat, Université d’Avignon et des Pays de Vaucluse, p 169.

Koffi E, Sea T, Dodehe Y, Soro S. 2010. Effect of solvent type on extraction of polyphenols from twenty-three Ivorian plants. J. Animal & Plant Sci. 5(3), 550-558.

Koudoro YA, Wotto DV, Konfo TRC, Agbangnan DCP, Sohounhloue CKD. 2015. Phytochemical screening, antibacterial and anti-radical activities of Daniellia oliveri trunk bark extracts used in veterinary médicine against gastrointestinal diseases in Benin. International Journal of Advanced Research 3(10), 1190-1198.

Kubola J, Siriamornpun S. 2008. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chemistry 110(4), 881-890.

Lagnika L. 2005. Etude phytochimique et activité biologique de substances naturelles isolées de plantes béninoises. Thèse de doctorat, Université Louis Pasteur Strasbourg, p 249.

Li HY, Hao ZB, Wanga XL, Lei Huang L, Li JP. 2009. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource Technology 100(2), 970-974.

https://doi.org/10.1016/j.biortech.2008.07.021

Mahmoudi S, Khali M, Mahmoudi N. 2013. Etude de l’extraction des composés phénoliques de différentes parties de la fleur d’artichaut (Cynara scolymus L.). Revue « Nature & Technologie ». B- Sciences Agronomiques et Biologiques 09, 35-40.

Millogo H. 2008. Etude des paramètres phytochimiques et des activités biologiques de Parkia biglobosa (Jacq) Benth (Mimosaceae). Thèse de Doctorat d’Etat es Sciences Naturelles, Université de Ouagadougou, p 169.

Mohammedi Z, Atik F. 2011. Impact of solvent extraction type on total polyphenols content and biological activity from Tamarix aphylla (L.) karst. Inter J Pharma Bio Sci 2(1), 609-615.

Mundi AD, Umar AZ, Musa YM, Adamu RS, Bamayi LJ. 2012. Preliminary Phytochemical Determination of Some Botanical Bark Powders. Publication of Nasarawa State University Keffi 8(1), 91-104.

Nacoulma OG. 1996. Plantes médicinales et Pratiques médicales Traditionnelles au Burkina Faso: cas du plateau central/ Tome II. Thèse d’Etat en Biochimie, Université de Ouagadougou (Burkina Faso), p 328.

Ouôba P, Lykke AM, Boussim J, Guinko S. 2006. La flore médicinale de la Forêt Classée de Niangoloko (Burkina Faso). Etudes flor. vég. Burkina Faso 10, 5-16.

Re R., Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicinal 26(9-10), 1231-1237.

https://doi.org/10.1016/s0891-5849(98)00315-3

Ribereau GP. 1968. Les composés phénoliques des végétaux. Dunod, Paris, 254 p.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalceu Reagent. Methods in Enzymology 299, 152-178.

Sripad G, Prakash V, Narasinga RMS. 1982. Extractability of polyphenols of sunflower seed in various solvents. Journal of Bioscience 4, 145-152.

Velazquez E, Tournier HA, Mordujovch de Buschhiazzo P, Saavedra G, Schinella GR. 2003. Antioxidant activity of Paraguayan plant extracts. Fitoterapia 74, 91-97.

Verma B, Hucl P, Chibbar RN. 2009. Phenolic acid composition and antioxidant capacity of acid and alkali hydrolysed wheat bran fractions. Food Chemistry 116, 947-954.

Zirihi G, Kra AKM, Guede-Guina F. 2003. Évaluation de l’activité antifongique deMicroglossa pyrifolia (Lamarck) O. Kantze (Astéracée) «PYMI» sur la croissance in vitro de Candida albicans. Revue de Médecine et pharmacie Afrique 17, 11- 18.

Windmi Kagambega, Lassina Ouattara, Crépin I. Dibala, Judith N. Semporé, Mamoudou H. Dicko.
Determination of phenolic compounds and evaluation of the antioxidant properties of four plants from Burkina Faso.
Int. J. Biosci. 17(1), 95-105, July 2020.
https://innspub.net/ijb/determination-phenolic-compounds-evaluation-antioxidant-properties-four-plants-burkina-faso/
Copyright © 2020
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update