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Effect of Plant Growth Promoting Rhizobacteria of the Growth of Cicer arietinum

By: Arooj Qaiser, Arsalan Fazal, Maryam Khan, Saba Shamim

Key Words: Plant growth promoting rhizobacteria (PGPR), Cicer arietinum L., Black chickpea, Biochemical tests, Bacillus velezensis.

Int. J. Biosci. 20(1),12-20, January 2022.

DOI: http://dx.doi.org/10.12692/ijb/20.1.12-20

Certification: ijb 2022 0017 [Generate Certificate]

Abstract

This research was conducted to isolate the rhizospheric bacteria of chickpea plant and to check their effect on its growth. Out of ten bacterial strains isolated, six were checked. They included two strains of Pseudomonas sp., three strains of Bacillus sp. and one strain of Brevibacterium sp. Out of all strains, one Bacillus strain showed good results. The 16s rRNA sequencing showed it Bacillus velezensis MN611255. Early germination, enhanced number of leaves, shoots, roots, and increase in their weight were notable features of B. velezensis as a PGPR. Furthermore, its effect on the flavonoids, total flavonoids, phenols, carbohydrates and chlorophyll content of chickpea plant was more pronounced as compared to the control. PGPR did not show siderophore production but were positive to indole acetic acid and phosphate solubilization. It can be concluded from the observations that indigenous isolated B. velezensis showed promising results as a PGPR. Field trials can help in further elaborating

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Effect of Plant Growth Promoting Rhizobacteria of the Growth of Cicer arietinum

Agbodjato NA, Noumavo PA, Baba-Moussa F, Salami HA, Sina H, Sèzan A, Bankolé H, Adjanohoun A, Baba-Moussa L. 2015. Characterization of potential plant growth promoting rhizobacteria Isolated from maize (Zea mays L.) in Central and Northern Benin (West Africa). Applied and Environmental Soil Science 2015, 1-9.

http://dx.doi.org/10.1155/2015/901656

Aron D. 1949. Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiology 24, 1-15.

http://dx.doi.org/10.1104/pp.24.1.1

Bric JM, Bostock RM, Silverstone SE. 1991. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane.  Applied and Environmental Microbiology 57, 535- 538.

Gaur PM, Tripathi S, Gowda CLL, Rao GVR, Sharma HC, Pande S, Sharma M. 2010. Chickpea seed production manual. Patancheru 502 324, Andra Pradesh, India. International Crops Research Institute for the Semi-Arid Tropics p 28.

Gopalakrishnan S, Vadlamudi S, Samineni S, Kumar CVS. 2016. Plant growth-promotion and biofortification of chickpea and pigeonpea through inoculation of biocontrol potential bacteria, isolated from organic soils. SpringerPlus 5(1), 1882-1893.

http://dx.doi.org/10.1186/s40064-016-3590-6

Hossain MA, Al- Raqmi KA, Al-Mijizy ZH, Weli

AM, Al- Riyami Q. 2013. Study of total flavonoids content and phytochemical screening of various leaves crude extracts of locally green Thymus vulgaris. Asian Pacific Journal of Tropical Biomedicine 3(9), 705-710.

http://dx.doi.org/10.1016/S2221-1691(13)60142-2

Hu QP, Xu JG. 2011. A simple double-layered chrome azurol S agar (SDCASA) plate assay to optimize the production of siderophores by a potential biocontrol agent Bacillus. African Journal of Microbiology Research 5(25), 4321-4327.

http://dx.doi.org/10.5897/AJMR11.238

Joseph B, Patra RR, Lawrence R. 2007. Characterization of plant growth promoting rhizobacteria associated with chickpea (Cicer arietinum L.). International Journal of Plant Production 1(2), 141-152.

http://dx.doi.org/10.22069/IJPP.2012.532

Kaun KB, Othman R, AbdulRahim K, Shamsuddin ZH. 2016. Plant growth promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilization of maize under greenhouse condition. PLoS One 11(3), 1-19.

http://dx.doi.org/10.1371/journal.pone.0152478

Kannahi M, Kowsalya M. 2013. Efficiency of plant growth promoting rhizobacteria for the enhancement Vigna mungo growth. Journal of Chemical and Pharmaceutical Research 5(5), 46-52.

Lewis G, Schrire B, MacKinder B, Lock M. 2005. Legumes of the world. Royal Botanical Gardens, Kew Publishing, UK.

Lwin KM, Myint MM, Tar T, Aung WZM. 2012. Isolation of plant hormone (indole-3-acetic acid- IAA) producing rhizobacteria and study on their effect on maize seedling. Engineering Journal 16(5), 137-144. http://dx.doi.org/10.4186/ej.2012.16.5.137

Morel MA, Brana V, Aung WZM. 2012. Legume crops, importance and use of bacterial inoculation to increase production, crop plant. Intech. p 217-241. http://dx.doi.org/10.5772/37413

Nautiyal CS. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170, 265-270.

http://dx.doi.org/10.1111/j.1574-6968.1999.tb13383.x

Pindi PK, Sultana S, Vootla PK. 2014. Plant growth regulation of Bt-cotton through Bacillus species. 3Biotech 4, 305-315.

http://dx.doi.org/10.1007/s13205-013-0154-0

Prabhavathi RM, Parsad MP, Jayaramu M. 2016. Studies on qualitative and quantitative phytochemical analysis of Cissus quadlangularis. Advances in Applied Science Research 7(4), 11-17.

Prathibha KA, Siddalingeshwara KG. 2013. Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescence as rhizobacteria on seed quality of sorghum. International Journal of Current Microbiology and Applied Sciences 2(3), 11-18.

Rahman AHM, Parvin MIA. 2014. Study of medicinal uses of Fabaceae family at Rajshahi, Bangladesh. Research in Plant Sciences 2(1), 6-8. http://dx.doi.org/10.12691/plant-2-1-2

Raaijmakers JM, Weller DM. 2002. Exploiting genotypic diversity of 2,4-diacetylphloroglucinol-producing Pseudomonas spp.: characterization of superior root-colonizing P. fluorescens strain Q8r1-96. Applied and Environmental Microbiology 56, 2545-2554.

http://dx.doi.org/10.1128/AEM.67.6.2545-2554.2001

Rajalakshmi K, Banu N. 2014. Extraction of chlorophyll from medicinal plants. International Journal of Chemical and Pharmaceutical Research 4(11), 209-212.

Rayavarapu VGB, Padmavathi T. 2016. Bacillus sp. as potential plant growth promoting rhizobacteria. International Journal of Advancements in Life Sciences 9(1), 29-36.

Silva-Beltrán NP, Ruiz-Cruz S, Cira-Chávez LA, Estrada-Alvarado MI, Ornelas-Paz JJ, López-Mata MA, Del-Toro-Sánchez CL, Ayala-Zavala JF, Márquez-Ríos E. 2015. Total phenolic, flavonoid, tomatine, and tomatidine contents and antioxidant and antimicrobial activities of extracts of tomato plant. International Journal of Analytical Chemistry 2015, 1-10.

http://dx.doi.org/10.1155/2015/284071

Yadav AN, Verna P, Singh B, Chauahan VS, Suman A, Saxena AK. 2017. Plant growth promoting bacteria: biodiversity and multifunctional attributes for sustainable agriculture. Advances in Biotechnology and Microbiology 5(5), 1-16.

http://dx.doi.org/10.19080/AIBM.2017.05.555671

Arooj Qaiser, Arsalan Fazal, Maryam Khan, Saba Shamim.
Effect of Plant Growth Promoting Rhizobacteria of the Growth of Cicer arietinum.
Int. J. Biosci. 20(1),12-20, January 2022.
https://innspub.net/ijb/effect-of-plant-growth-promoting-rhizobacteria-of-the-growth-of-cicer-arietinum/
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