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Morphology of erythrocyte and hematological parameters of red tilapia (Oreochromis sp.) after challenge with Streptococcus agalactiae

By: Van-Thanh Vo, Thi-Hieu Tran, Thi-Tu-Quyen Huynh, Huyen Nguyen Thi Thuong

Key Words: Hematological parameters, Morphology of erythrocyte, Streptococcus agalactiae, Red tilapia.

Int. J. Biosci. 20(1),116-122, January 2022.


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The study focused on investigating the morphology of erythrocyte and some hematological parameters (including Hct index, Hb value, RBC count, WBC & thrombocyte count) on red tilapia (Oreochromis sp.) infected Streptococcus agalactiae before infection (control), five days post-infection and ten days post-infection. The experiment was arranged with two treatments containing control treatment and infection treatment. Each treatment has 3 replications with 3 tanks/treatment (n=10 fish). In this study, the hematological indexes both enhanced while the size of the erythrocyte reduced over five days and ten days of infection. Immature erythrocytes and abnormal erythrocytes are abundantly present in the peripheral blood of infected red tilapia, explaining the decrease in mean erythrocyte size and the RBC count increases.

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Morphology of erythrocyte and hematological parameters of red tilapia (Oreochromis sp.) after challenge with Streptococcus agalactiae

Alsaid M, Abuseliana AF, Daud HH, Mustapha NM, Bejo SK, Abdelhadi YM, Hamdan RH. 2014. Haematological, biochemical and clinical signs changes following experimental infection of Streptococcus agalactiae in red hybrid tilapia (Oreochromis sp.). Aquacultura Indonesiana 15(2), 86–93.

Amal NAM, Zamri-Saad M. 2011. Streptococcosis in Tilapia (Oreochromis niloticus): A review. Pertanika Journal of Tropical Agricultural Science 34(2), 195–206.

Boerlage AS, Dung TT, Hoa TTT, Davidson J, Stryhn H, Hammell KL. 2017. Production of red tilapia (Oreochromis spp.) in floating cages in the Mekong Delta, Vietnam: mortality and health management. Diseases of Aquatic Organisms 124, 131–144.

Bondad-Reantaso MG, Subasinghe RP, Arthur JR, Ogawa K, Chinabut S, Adlard R, Tan Z, Shariff M. 2005. Disease and health management in Asian aquaculture. Veterinary Parasitology 132(3–4), 249–272.

Campbell TW, Ellis CK. 2007. Avian and Exotic Animal Hematology and Cytology (Third Edition). Iowa State University Press.

Clauss TM, Dove ADM, Arnold JE. 2008. Hematologic Disorders of Fish. Veterinary Clinics of North America: Exotic Animal Practice 11(3), 445–462.

Duncan PL, Lovell RT. 1994. Influence of vitamin C on the folate requirement of channel catfish, Ictalurus punctatus, for growth, hematopoiesis, and resistance to Edwardsiella ictaluri infection. Aquaculture 127(2–3), 233–244.

FAO. 2020. 2020. FAO.

Farahi A, Kasiri M, Sudagar M, Iraei MS, Shahkolaei MD. 2010. Effect of garlic (Allium sativum) on growth factors, some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss). AACL Bioflux 3(4), 317–323.

Groff JM, Zinkl JG. 1999. Hematology and Clinical Chemistry of Cyprinid Fish: Common Carp and Goldfish. Veterinary Clinics of North America: Exotic Animal Practice 2(3), 741–776.

Guo JJ, Kuo CM, Chuang YC, Hong JW, Chou RL, Chen TI. 2012. The effects of garlic-supplemented diets on antibacterial activity against Streptococcus iniae and on growth in orange-spotted grouper, Epinephelus coioides. Aquaculture 364–365, 33–38.

Hrubec TC, Cardinale JL, Smith SA. 2000. Hematology and plasma chemistry reference intervals for cultured Tilapia Oreochromis hybrid. Veterinary Clinical Pathology 29(1), 7–12.

Huu Thinh Nguyen, Kanai K, Yoshikoshi K. 2001. Experimental Streptococcus iniae infection in Japanese Flounder Paralichthys olivaceus. Fish Pathology 36, 40–41.

Klesius PH, Shoemaker CA, Evans JJ. 2008. Streptococcus: A worldwide fish health problem. 8th International Symposium on Tilapia in Aquaculture 83–107.

Martins ML, Vieira FN, Jerônimo GT, Mouriño JLP, Dotta G, Speck GM, Bezerra AJM, Pedrotti FS, Buglione-Neto CC, Pereira G. 2009. Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with Enterococcus sp. Fish Physiology and Biochemistry 35(1), 219–222.

Miao W, Wang W. 2020. Trends of Aquaculture Production and Trade: Carp, Tilapia, and Shrimp. Asian Fisheries Science 33S.

Miwa S, Inouye K. 1999. Histopathological Study of the Flounder with Anemia Found in Various Places in Japanese Coastal Waters. Fish Pathology 34(3), 113–119.

Mjoun K, Rosentrater K, Brown ML. 2010. TILAPIA: Profile and Economic Importance. Fact Sheets.

Natt MP, Herrick CA. 1952. A New Blood Diluent for Counting the Erythrocytes and Leucocytes of the Chicken. Poultry Science 31(4), 735–738.

Nya EJ, Austin B. 2009. Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum).Journal of Fish Diseases 32(11), 963–970.

Subasinghe RP. 2005. Epidemiological approach to aquatic animal health management: opportunities and challenges for developing countries to increase aquatic production through aquaculture. Preventive Veterinary Medicine 67, 117–124.

Suwannasang A, Dangwetngam M, Issaro A, Phromkunthong W, Suanyuk N. 2014. Pathological manifestations and immune responses of serotypes Ia and III Streptococcus agalactiae infections in Nile tilapia (Oreochromis niloticus). 8.

Tavares-Dias M, Moraes FR de, Martin ML, Santana ÁE. 2002. Haematological changes in Oreochromis niloticus (Osteichthyes: Cichlidae) with gill Ichthyophthiriasis and Saprolegniosis. Fisheries Institute Bulletin, São Paulo 28(1), 1–9.

Van-Thanh Vo, Thi-Hieu Tran, Thi-Tu-Quyen Huynh, Huyen Nguyen Thi Thuong.
Morphology of erythrocyte and hematological parameters of red tilapia (Oreochromis sp.) after challenge with Streptococcus agalactiae.
Int. J. Biosci. 20(1),116-122, January 2022.
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