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Salicylic acid pre-treatment effects on Beta vulgaris L. multigerm germination and germination indices

By: Sandip Palve, Digambar Ahire, Yogesh Gahile

Key Words: Germination percentage, Height reduction, Relative injury rate, Salicylic acid, Seed vigor.

Int. J. Biosci. 20(1),59-71, January 2022.

DOI: http://dx.doi.org/10.12692/ijb/20.1.59-71

Certification: ijb 2022 0022 [Generate Certificate]

Abstract

The response of Beta vulgaris L. seeds to pre-treatment with salicylic acid (SA) was investigated at the early seedling stage and focused on germination indices to evaluate seed quality at a particular treatment. To achieve this goal, beet seeds were treated with various concentrations of salicylic acid (0.4, 0.8, 1.2, 1.6, 2.0, 2.4, 2.8, 3.2, 3.6, 4.0, 4.4, 4.8, 5.2, 5.6, 6.0 and 6.4 mM) along with control. The experiment was conducted with a dark red variety of beet seeds. Seeds exhibited more tolerance up to 2.0 mM SA treatment. High concentrations (>2.0 mM) of SA delayed the mean germination time of seeds and increased relative injury rate and seedling height reduction. However, germination percentage, germination index, seed vigor, water uptake percentage reduced significantly. Total inhibition of seed germination was found at 5.2 to 6.4 mM SA treatment. Rudimentary seedling growth was observed from 3.6 mM SA to further treatments. From morphological results, this study proposes that up to 2.0 mM of salicylic acid treatment may be helpful in seed germination and seedling growth of the beet. Also, this study might be helpful for further research on the influence of salicylic acid on the growth and development of beets.

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Salicylic acid pre-treatment effects on Beta vulgaris L. multigerm germination and germination indices

Dempsey DMA, Shah J, Klessig DF. 1999. Salicylic acid and disease resistance in plants. Critical Reviews in Plant Sciences 18(4), 547-575.

Durrant WE, Dong X. 2004. Systemic acquired resistance. Annual Review of Phytopathology 42, 185-209.

Dempsey DMA, Klessig DF. 2017. How does the multifaceted plant hormone salicylic acid combat disease in plants, and are similar mechanisms utilized in humans?. BMC biology 15(1), 1-11.

Achard P, Cheng H, De Grauwe L, Decat J, Schoutteten H, Moritz T, Harberd NP. 2006. Integration of plant responses to environmentally activated phytohormonal signals. Science 311(5757), 91-94.

Vlot AC, Dempsey DMA, Klessig DF. 2009. Salicylic acid, a multifaceted hormone to combat disease. Annual review of phytopathology 47, 177-206.

Wolters H, Jürgens G. 2009. Survival of the flexible: hormonal growth control and adaptation in plant development. Nature Reviews Genetics 10(5), 305-317.

Ariizumi T, Steber CM. 2007. Seed germination of GA-insensitive sleepy1 mutants does not require RGL2 protein disappearance in Arabidopsis. The Plant Cell, 19(3), 791-804.

Robert C, Noriega A, Tocino Á, Cervantes E. 2008. Morphological analysis of seed shape in Arabidopsis thaliana reveals altered polarity in mutants of the ethylene signaling pathway. Journal of plant physiology 165(9), 911-919.

Seo M, Nambara E, Choi G, Yamaguchi S. 2009. Interaction of light and hormone signals in germinating seeds. Plant molecular biology 69(4), 463.

Borsani O, Valpuesta V, Botella MA. 2001. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiology 126, 1024–1030.

Rajjou L, Belghazi M, Huguet R, Robin C, Moreau A, Job C, Job D. 2006. Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. Plant Physiology 141(3), 910-923.

Alonso-Ramırez A, Rodrıguez D, Reyes D. 2009. Jimé nez JA, Nicolá s G, Ló pez-Climent M, et al. Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds. Plant Physiol 150(3), 1335-44.

Khan MIR, Fatma M, Per TS, Anjum NA, Khan NA. 2015. Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants. Frontiers in plant science 6, 462.

Malamy J, Klessig DF. 1992. Salicylic acid and plant disease resistance. The Plant Journal 2(5), 643-654.

Hayat Q, Hayat S, Irfan M, Ahmad A. 2010. Effect of exogenous salicylic acid under changing environment: a review. Environmental and experimental botany 68(1), 14-25.

Rivas-San Vicente M, Plasencia J. 2011. Salicylic acid beyond defense: its role in plant growth and development. Journal of experimental botany 62(10), 3321-3338.

Miura K, Tada Y. 2014. Regulation of water, salinity, and cold stress responses by salicylic acid. Frontiers in plant science 5, 4.

Guan L, Scandalios JG. 1995. Developmentally related responses of maize catalase genes to salicylic acid. Proceedings of the National Academy of Sciences 92(13), 5930-5934.

Nishimura N, Kitahata N, Seki M, Narusaka Y, Narusaka M, Kuromori T, Hirayama T. 2005. Analysis of ABA hypersensitive germination2 revealed the pivotal functions of PARN in stress response in Arabidopsis. The Plant Journal 44(6), 972-984.

Xie Z, Zhang ZL, Hanzlik S, Cook E, Shen QJ. 2007. Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid-inducible WRKY gene. Plant molecular biology 64(3), 293-303.

Gairola KC, Nautiyal AR, Dwivedi AK. 2011. Effect of temperatures and germination media on seed germination of Jatropha curcas Linn. Advances in bioresearch 2(2), 66-71.

Li Y. 2008. Effect of salt stress on seed germination and seedling growth of three salinity plants. Pakistan journal of biological sciences 11(9), 1268-1272.

Ellis RH, Roberts EH. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology (Netherlands).

AbdulBaki AA, Anderson JD. 1973. Vigor determination in soybean seed by multiple criteria 1. Crop science 13(6), 630-633.

Tsegay BA, Gebreslassie B. 2014. The effect of salinity (NaCl) on germination and early seedling growth of Lathyrus sativus and Pisum sativum var. abyssinicum. African Journal of Plant Science 8(5), 225-231.

Islam MM, Karim MA. 2010. Evaluation of rice (Oryza sativa L.) genotypes at germination and early seedling stage for their tolerance to salinity. The agriculturists 8(2), 57-65.

Yousof FI, El-Saidy AEA. 2014. Application of salicylic acid to improve seed vigor and yield of some bread wheat cultivars (Triticum aestivum L.) under salinity stress. Research Journal of Seed Science 7(2), 52-62.

Obroucheva NV, Antipova OV. 2004. The role of water uptake in the transition of recalcitrant seeds from dormancy to germination. Russian Journal of Plant Physiology 51(6), 848-856.

Anaya F, Fghire R, Wahbi S, Loutfi K. 2018. Influence of salicylic acid on seed germination of Vicia faba L. under salt stress. Journal of the Saudi Society of Agricultural Sciences 17(1), 1-8.

Bahrani A, Pourreza J. 2012. Gibberellic acid and salicylic acid effects on seed germination and seedlings growth of wheat (Triticum aestivum L.) under salt stress conditions. World Applied Science Journal 18(5), 633-641.

Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova DR. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant science 164(3), 317-322.

Wu L, Guo X, Harivandi MA. 1998. Allelopathic effects of phenolic acids detected in buffalograss (Buchloe dactyloides) clippings on the growth of annual bluegrass (Poa annua) and buffalograss seedlings. Environmental and Experimental Botany 39(2), 159-167.

Guan L, Scandalios JG. 1995. Developmentally related responses of maize catalase genes to salicylic acid. Proceedings of the National Academy of Sciences 92(13), 5930-5934.

McCue P, Zheng Z, Pinkham L, Shetty K. 2000.A model for enhanced pea seedling vigor following low pH and salicylic acid treatments. Process Biochemistry 35(6), 603-613.

De Los Reyes BG, Myers SJ, McGrath JM. 2003. Differential induction of glyoxylate cycle enzymes by stress as a marker for seedling vigor in sugar beet (Beta vulgaris). Molecular Genetics and Genomics 269(5), 692-698.

Halmer P. 2004. Methods to improve seed performance in the field. Handbook of seed physiology 125-65.

Patel PK, Hemantaranjan A. 2013. Differential sensitivity of chickpea genotypes to salicylic acid and drought stress during preanthesis: effects on total chlorophyll, phenolics, seed protein, and protein profiling. The Bioscan 8(2), 569-574.

Lin KH, Huang SB, Wu CW, Chang YS. 2019. Effects of salicylic acid and calcium chloride on heat tolerance of poinsettia. Hortscience 54(3), 499-504.

Li Z, Xu J, Gao Y, Wang C, Guo G, Luo Y, Hu J. 2017. The synergistic priming effect of exogenous salicylic acid and H2O2 on chilling tolerance enhancement during maize (Zea mays L.) seed germination. Frontiers in plant science 8, 1153.

Yaldagard M, Mortazavi SA, Tabatabaie F. 2008. Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. African Journal of Biotechnology 7(14).

Ahmad F, Iqbal S, Khan MR, Abbas MW, Ahmad J, Nawaz H, Ali M. 2019. 22. Influence of seed priming with salicylic acid on germination and early growth of sesame. Pure and Applied Biology (PAB), 8(2), 1206-1213.

Alamri SA, Siddiqui MH, Al-Khaishani MY, Ali HM. 2018. Response of salicylic acid on seed germination and physio-biochemical changes of wheat under salt stress. Acta Scientific Agriculture 2(5), 36-42.

Liu L, Xia W, Li H, Zeng H, Wei B, Han S, Yin C. 2018. Salinity inhibits rice seed germination by reducing α-amylase activity via decreased bioactive gibberellin content. Frontiers in Plant Science 9, 275.

Gossett DR, Millhollon EP, Lucas MC. 1994. Antioxidant response to NaCl stress in salt‐tolerant and salt‐sensitive cultivars of cotton. Crop science 34(3), 706-714.

Ma X, Zheng J, Zhang X, Hu Q, Qian R. 2017. Salicylic acid alleviates the adverse effects of salt stress on Dianthus superbus (Caryophyllaceae) by activating photosynthesis, protecting morphological structure, and enhancing the antioxidant system. Frontiers in plant science 8, 600.

Jini D, Joseph B. 2017. Physiological mechanism of salicylic acid for alleviation of salt stress in rice. Rice Science 24(2), 97-108.

Boukraâ D, Benabdelli K, Belabid L, Bennabi, F. 2013. Effect of salinity on chickpea seed germination pre-treated with salicylic acid. Scientific Journal of Biology Science 2(4), 86-93.

Szalai G, Páldi E, Janda T. 2005. Effect of salt stress on the endogenous salicylic acid content in maize (Zea mays L.) plants. Acta Biologica Szegediensis 49(1-2), 47-48.

Jam BJ, Shekari FARID, Azimi MR, Zangani, Esmaeil. 2012. Effect of priming by salicylic acid on germination and seedling growth of safflower seeds under CaCl2 stress. International Journal of Agricultural Research 2, 1097-1105.

Fariduddin Q, Hayat S, Ahmad A. 2003. Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity, and seed yield in Brassica juncea. Photosynthetica 41(2), 281-284.

Kaur S, Gupta AK, Kaur N. 2002. Effect of Osmo-and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant growth regulation 37(1), 17-22.

Baninasab B. 2010. Induction of drought tolerance by salicylic acid in seedlings of cucumber (Cucumis sativus L.). The Journal of Horticultural Science and Biotechnology 85(3), 191-196.

El-Tayeb MA. 2005. Response of barley grains to the interactive e. ect of salinity and salicylic acid. Plant growth regulation 45(3), 215-224.

Sandip Palve, Digambar Ahire, Yogesh Gahile .
Salicylic acid pre-treatment effects on Beta vulgaris L. multigerm germination and germination indices.
Int. J. Biosci. 20(1),59-71, January 2022.
https://innspub.net/ijb/salicylic-acid-pre-treatment-effects-on-beta-vulgaris-l-multigerm-germination-and-germination-indices/
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