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Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions

By: Maiada Mohammed El-Dawayati, Hesham Sayed Ghazzawy, Muhammad Munir

Key Words: Date palm, Embryogenesis, Polyamines, Putrescine, Spermidine.

Int. J. Biosci. 12(1), 149-159, January 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.1.149-159

Certification: ijb 2018 0197 [Generate Certificate]

Abstract

Date palm is traditionally propagated by ground off-shoots, which are in a limited number and varied with the cultivar. The production of date palm through tissue culture is well established due to the advantages of rapid clonal production, genetically uniformity and pathogen-free plants. The source explants are grown in a solid or liquid media. The media formulation and amendments play a vital role to establish a successful tissue culture protocol for explant regeneration and multiplication. Therefore, the rational of present study was to enhance somatic embryos induction of date palm cv. Sewi from embryonic callus during maturation stage by adding polyamines and glutamine amino acid concentrations in solid or liquid media. Two types of polyamines, putrescine and spermidine (each at 100 mg.L-1 concentration) along with basic concentration of glutamine amino acid (B) (200 mg.L-1) or with the extra addition of glutamine concentration (EA) (100 mg.L-1) were included in the study to develop micropropagation cycle of date palm plantlets. Explants cultured on solid media showed excellent embryonic callus whereas liquid media was best for somatic embryos induction. The quantitative observation indicated that maximum number of somaticembryos (32.33) were counted in liquid mediacontaining glutamine (EA) with putrescine. On the other hand, highest embryonic callus fresh weight was produced by the explants contained spermidinealone (5.49 g) and glutamine (EA) + spermidine (5.30 g) in solid media. It was concluded that putrescine with glutamine (EA) orits combination with spermidinealong with glutamine (B) in the solid or liquid in-vitromedia were the best for embryonic callus maturation to enhance the somatic embryogenesis of date palm cv. Sewi.

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Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions

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Maiada Mohammed El-Dawayati, Hesham Sayed Ghazzawy, Muhammad Munir.
Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions.
Int. J. Biosci. 12(1), 149-159, January 2018.
https://innspub.net/ijb/somatic-embryogenesis-enhancement-date-palm-cultivar-sewi-using-different-types-polyamines-glutamine-amino-acid-concentration-vitro-solid-liquid-media-conditions/
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