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Thermal stability of two xylanases from Macrotermes subhyalinus little soldier: kinetic and thermodynamic analysis

By: Jean Bedel Fagbohoun, Mankambou Jacques Gnanwa, Detto Karamoko Bi Eugene Toma Zan, Soumaila Dabonne, Lucien Patrice Kouame

Key Words: Xylanases, Kinetic parameters, Macrotermes subhyalinus, Thermodynamic parameters

Int. J. Biosci. 12(1), 65-75, January 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.1.65-75

Certification: ijb 2018 0187 [Generate Certificate]

Abstract

The knowledge on thermal inactivation kinetics of enzymes is necessary to allow their adequate utilization as natural biopreservatives in the food industry and technology applications. In this work, the kinetics of thermal inactivation was studied for the previously purified and characterized xylanases Xyl1 and Xyl2 from Macrotermes subhyalinus little soldier. Samples of xylanases were treated at different time-temperature combinations in the range of 5-60 min at 50-65°C and the kinetic and thermodynamic parameters for xylanases were calculated. The inactivation kinetic follows a first-order reaction with k-values between 0.0192 ± 0.0002 to 0.0405 ± 0.0003 and 0.0119 ± 0.0005 to 0.0418 ± 0.0004 min-1 for Xyl1 and Xyl2, respectively. Activation energy (Ea) and Z-values were estimated to 48.08 ± 1.84 kJ mol-1 and 43.47 ± 3.02°C for Xyl1, 75.52 ± 3.52 kJ.mol-1 and 27.77 ± 1.87°C for Xyl2. The catalytic reactions of both xylanases are endothermic due to positive enthalpy. The high value obtained for the variation in enthalpy of activation indicates that a high amount of energy is required to initiate denaturation, probably due to the molecular conformation of xylanases. All results suggest that both xylanases are relatively resistant to long heat treatments up to 50°C.

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Thermal stability of two xylanases from Macrotermes subhyalinus little soldier: kinetic and thermodynamic analysis

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Jean Bedel Fagbohoun, Mankambou Jacques Gnanwa, Detto Karamoko Bi Eugene Toma Zan, Soumaila Dabonne, Lucien Patrice Kouame.
Thermal stability of two xylanases from Macrotermes subhyalinus little soldier: kinetic and thermodynamic analysis.
Int. J. Biosci. 12(1), 65-75, January 2018.
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