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Toxigenic diphtheria: A review of the current knowledge

By: Rehman Ali, Marina Khan, Muhammad Rooman

Key Words: Corynebacterium, Diphtheria, Pathogenesis, Exotoxin, Phospholipase D, Diphtheria toxin

Int. J. Biosci. 16(3), 518-535, March 2020.

DOI: http://dx.doi.org/10.12692/ijb/16.3.518-535

Certification: ijb 2020 0099 [Generate Certificate]

Abstract

Toxigenic diphtheria is the most feared and vaccine-preventable disease of global importance. It is one of the major health problems in developing countries and causes high morbidity and mortality. Three distinct species (Corynebacterium ulcerans, C. pseudotuberculosis, and C. diphtheriae) of the renowned genus Corynebacterium are responsible for this pathological condition. The review deals with the current advance knowledge concerning the etiology, transmission, distribution, pathogenesis, and control of the three pathogens. These pathogenic bacteria produce highly potent exotoxins: the major virulence factors. C. diphtheriae causes respiratory and cutaneous diphtheria most commonly in small children. C. pseudotuberculosis and C. ulcerans are the etiological agents of caseous lymphadenitis and zoonotic diphtheria, respectively. These species share particular features like specific cell wall composition (peptidoglycan, arabinogalactan and mycolic acid) and high G+C contents. These microorganisms are of considerable importance regarding the medical, veterinary, and biotechnological point of view. The toxins produced by toxigenic strains are highly potent and disseminate quickly, once disseminated then they could be highly lethal. Thus diphtheria toxoid and diagnosis followed by prompt treatment is the only way to decrease the rate of morbidity as well as mortality.

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Toxigenic diphtheria: A review of the current knowledge

Alcano EI. 1997. Fundamentals of Microbiology (5th Edition ed.): The Benjamin/Cummings Publisihing Company.

Ali R, Awan Z-u-R, Awan M-u-R. 2018. Epidemiology of Diphtheria in genral population of Bannu division and IDPs of North Waziristan Agency (NWA), Pakistan. FUUAST Journal of Biology 8(2), 305-310.

Allen CE, Schmitt MP. 2009. HtaA is an iron-regulated hemin binding protein involved in the utilization of heme iron in Corynebacterium diphtheriae. Journal of Bacteriology 191(8), 2638-2648.

Almeida S, Sousa C, Abreu V, Diniz C, Dorneles E, Lage AP, Barh D, Azevedo V. 2017. Exploration of nitrate Reductase metabolic pathway in Corynebacterium pseudotuberculosis. International journal of genomics.

Augustine JL, Renshaw H. 1986. Survival of Corynebacterium pseudotuberculosis in axenic purulent exudate on common barnyard fomites. American Journal of Veterinary Research 47(4), 713-715.

Barret N. 1986. Communicable disease assocaited with milk and dairy products in England and Wales: 1983-1984. Journal of Infection 12(3), 265-272.

Berger A, Lensing C, Konrad R, Huber I, Hogardt M, Sing A. 2012. Sexually transmitted diphtheria. Sexually Transmitted Infections, sextrans-2011-050418.

Bonmarin I, Guiso N, Le Flèche-Matéos A, Patey O, Patrick AG, Levy-Bruhl D. 2009. Diphtheria: a zoonotic disease in France? Vaccine 27(31), 4196-4200.

Bostock A, Gilbert F, Lewis D, Smith D. 1984. Corynebacterium ulcerans infection associated with untreated milk. Journal of Infection 9(3), 286-288.

Both L, Neal S, De Zoysa A, Mann G, Czumbel I, Efstratiou A. 2014. External quality assessments for microbiologic diagnosis of diphtheria in Europe. Journal of Clinical Microbiology 52(12), 4381-4384.

Broadway MM, Rogers EA, Chang C, Huang IH, Dwivedi P, Yildirim S, Schmitt MP, Das A, Ton-That H. 2013. Pilus gene pool variation and the virulence of Corynebacterium diphtheriae clinical isolates during infection of a nematode. Journal of Bacteriology 195(16), 3774-3783.

CDC. 2015. Epidemiology and prevention of vaccine preventable disease: Center for Disease Control and Prevention.

CerdeñoTárraga A, Efstratiou A, Dover L, Holden M, Pallen M, Bentley S, Besra G, Churcher C, James K, De Zoysa A. 2003. The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129. Nucleic Acids Research 31(22), 6516-6523.

Cetinkaya B, Karahan M, Atil E, Kalin R, De Baere T, Vaneechoutte M. 2002. Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR. Veterinary Microbiology 88(1), 75-83.

Chang C, Mandlik A, Das A, TonThat H. 2011. Cell surface display of minor pilin adhesins in the form of a simple heterodimeric assembly in Corynebacterium diphtheriae. Molecular Microbiology 79(5), 1236-1247.

Colombo AV, Hirata Jr R, de Souza CMR, Monteiro-Leal LH, Previato JO, Formiga LCD, Andrade AFB, Mattos-Guaraldi AL. 2001. Corynebacterium diphtheriae surface proteins as adhesins to human erythrocytes. FEMS Microbiology Letters 197(2), 235-239.

De Carpentier J, Flanagan P, Singh I, Timms M, Nassar W. 1992. Nasopharyngeal Corynebacterium ulcerans: a different diptheria. The Journal of Laryngology & Otology 106(9), 824-826.

de Mattos Guaraldi AL, Hirata R, de Carvalho Azevedo VA. 2014. Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis—general aspects Corynebacterium diphtheriae and Related Toxigenic Species (pp. 15-37).

de Oliveira Moreira L, Andrade AFB, Vale MD, Souza SMS, Hirata R, Asad LMOB, Asad NR, Monteiro-Leal LH, Previato JO, Mattos-Guaraldi AL. 2003. Effects of iron limitation on adherence and cell surface carbohydrates of Corynebacterium diphtheriae strains. Applied and Environmental Microbiology 69(10), 5907-5913.

De Zoysa A, Hawkey PM, Engler K, George R, Mann G, Reilly W, Taylor D, Efstratiou A. 2005. Characterization of toxigenic Corynebacterium ulcerans strains isolated from humans and domestic cats in the United Kingdom. Journal of Clinical Microbiology 43(9), 4377-4381.

Dias AAdSd, Santos LS, Sabbadini PS, Santos CS, Junior S, Correa F, Napoleão F, Nagao PE, Villas-Bôas MHS, Hirata Junior R. 2011. Corynebacterium ulcerans diphtheria: an emerging zoonosis in Brazil and worldwide. Revista de Saúde Publica 45(6), 1176-1191.

Divino-Goes K, de Moraes-Pinto M, Dinelli M, Casagrande S, Bonetti T, Andrade P, Weckx L. 2007. Prevalence of diphtheria and tetanus antibodies and circulation of Corynebacterium diphtheriae in São Paulo, Brazil. Brazilian Journal of Medical and Biological Research 40(12), 1681-1687.

Dorella FA, Pacheco LGC, Oliveira SC, Miyoshi A, Azevedo V. 2006. Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Veterinary Research 37(2), 201-218.

Efstratiou A, Engler KH, Dawes CS, Sesardic D. 1998. Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic Corynebacteria. Journal of Clinical Microbiology 36(11), 3173-3177.

Efstratiou A, Engler KH, Mazurova IK, Glushkevich T, Vuopio-Varkila J, Popovic T. 2000. Current approaches to the laboratory diagnosis of diphtheria. Journal of Infectious Diseases 181(Supplement_1), S138-S145.

Efstratiou A, George R. 1999. Laboratory guidelines for the diagnosis of infections caused by Corynebacterium diphtheriae and C. ulcerans. Communicable Disease and Public Health 2, 250-257.

Engler K, Efstratiou A, Norn D, Kozlov R, Selga I, Glushkevich T, Tam M, Melnikov V, Mazurova I, Kim V. 2002. Immunochromatographic strip test for rapid detection of diphtheria toxin: description and multicenter evaluation in areas of low and high prevalence of diphtheria. Journal of Clinical Microbiology 40(1), 80-83.

Gaspar AH, Ton-That H. 2006. Assembly of distinct pilus structures on the surface of Corynebacterium diphtheriae. Journal of Bacteriology 188(4), 1526-1533.

Gilbert R, Stewart. 1926. Corynebacterium ulcerans: a pathogenic microorganism resembling Corynebacterium diphtheriae. Journal of Laboratory and Clinical Medicine 12, 756-761.

Guiso N. 2015. Impact of vaccination on epidemiology of diphtheria and pertussis. Vaccine Research 2(1), 6-8. doi: 10.18869/ acadpub. vacres.

Hadfield TL, McEvoy P, Polotsky Y, Tzinserling VA, Yakovlev AA. 2000. The pathology of diphtheria. Journal of Infectious Diseases 181, 116-120.

Hantke K. 2001. Iron and metal regulation in bacteria. Current Opinion in Microbiology 4(2), 172-177.

Hatanaka A, Tsunoda A, Okamoto M, Ooe K, Nakamura A, Miyakoshi M, Komiya T, Takahashi M. 2003. Corynebacterium ulcerans Diphtheria in Japan. Emerging Infectious Diseases 9(6), 752-753.

Henrisksen S. 1955. Some bacteriophages of Corynebacterium ulcersans and their lack of effect on toxigenicity. Acta Pathologica Microbiologica Scandinavica 37(1), 65-70.

Holmes RK. 2000. Biology and molecular epidemiology of diphtheria toxin and the tox gene. Journal of Infectious Diseases 181, 156-167.

Jamal S, Tiwari S, Silva A, Azevedo V. 2017. Pathogenesis of Corynebacterium diphtheriae and available vaccines; an overview. Glob J Infect Dis Clin Res 3(1), 020-024.

Join-Lambert OF, Ouache M, Canioni D, Beretti J-L, Blanche S, Berche P, Kayal S. 2006. Corynebacterium pseudotuberculosis necrotizing lymphadenitis in a twelve-year-old patient. The Pediatric infectious disease journal 25(9), 848-851.

Karakus R, Caglar K, Aybay C. 2007. Evaluation of human antibody responses to diphtheria toxin subunits A and B in various age groups. Clinical Microbiology and Infection 13(11), 1065-1071.

Kaufmann D, Ott P, Zbinden R. 2002. Laryngopharyngitis by Corynebacterium ulcerans. Infection 30(3), 168-170.

Keslin MH, McCoy EL, McCusker JJ, Lutch JS. 1979. Corynebacterium pseudotuberculosis: a new cause of infectious and eosinophilic pneumonia. The American journal of medicine 67(2), 228-231.

Kolodkina V, Titov L, Sharapa T, Grimont F, Grimont PA, Efstratiou A. 2006. Molecular epidemiology of C. diphtheriae strains during different phases of the diphtheria epidemic in Belarus. BMC Infectious Diseases 6(1), 129.

Kolybo D, Labyntsev A, Korotkevich N, Komisarenko S, Romaniuk S, Oliinyk O. 2013. Immunobiology of diphtheria. Recent approaches for the prevention, diagnosis, and treatment of disease. Biotechnologia Acta 6(4).

Kraeva L, ZhN M, Radchenko A. 2007. Etiologic role of Corynebacterium non diphtheriae in patients with different pathology. Zhurnal mikrobiologii, epidemiologii, i immunobiologii (5), 3-7.

Labyntsev А, Kolybo D, Yurchenko E, Kaberniuk A, Korotkevych N, Komisarenko S. 2014. Effect of the T-domain on intracellular transport of diphtheria toxin. Ukrainian biochemical journal (86,№ 3), 77-87.

Liu D, Chan W, Fan D, Lam D. 2005. An infected hydrogel buckle with Corynebacterium pseudotuberculosis. British Journal of Ophthalmology 89(2), 245-246.

Liu Y, Li SY. 2009. Urokinase-targeted recombinant bacterial protein toxins—a rationally designed and engineered anticancer agent for cancer therapy. Frontiers of Biology in China 4(1), 1-6.

Lopez J, Wong F, Quesada J. 1966. Corynebacterium pseudotuberculosis. First case of human infection. American Journal of Clinical Pathology 46(5), 562-567.

Maheriya K, Pathak GH, Chauhan AV, Mehariya MK, Agrawal PC. 2014. Clinical and epidemiological profile of diphtheria in Tertiary Care Hospital. Gujarat Medical Journal 69(2), 105-108.

Mandlik A, Das A, Ton-That H. 2008a. The molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria. Proceedings of the National Academy of Sciences 105(37), 14147-14152.

Mandlik A, Swierczynski A, Das A, Ton-That H. 2008b. Pili in Gram-positive bacteria: assembly, involvement in colonization and biofilm development. Trends in Microbiology 16(1), 33-40.

Mandlik A, Swierczynski A, Das A, TonThat H. 2007. Corynebacterium diphtheriae employs specific minor pilins to target human pharyngeal epithelial cells. Molecular Microbiology 64(1), 111-124.

Massenburg D, Han JS, Liyanage M, Patton WA, Rhee SG, Moss J, Vaughan M. 1994. Activation of rat brain phospholipase D by ADP-ribosylation factors 1, 5, and 6: separation of ADP-ribosylation factor-dependent and oleate-dependent enzymes. Proceedings of the National Academy of Sciences 91(24), 11718-11722.

Mattos-Guaraldi AL, Moreira LO, Damasco PV, Hirata Júnior R. 2003. Diphtheria remains a threat to health in the developing world: an overview. Memorias do Instituto Oswaldo Cruz 98(8), 987-993.

Maximescu P, Oprisan A, Pop A, Potorac E. 1974. Further studies on Corynebacterium species capable of producing diphtheria toxin (C. diphtheriae, C. ulcerans, C. ovis). Microbiology 82(1), 49-56.

McKean SC, Davies JK, Moore RJ. 2007. Expression of phospholipase D, the major virulence factor of Corynebacterium pseudotuberculosis, is regulated by multiple environmental factors and plays a role in macrophage death. Microbiology 153(7), 2203-2211. doi: doi:10.1099/mic.0.2007/005926-0

Meera M, Rajarao M. 2014. Diphtheria in Andhra Pradesh–a clinical-epidemiological study. International Journal of Infectious Diseases 19, 74-78.

Meinel DM, Konrad R, Berger A, König C, Schmidt-Wieland T, Hogardt M, Bischoff H, Ackermann N, Hörmansdorfer S, Krebs S. 2015. Zoonotic transmission of toxigenic Corynebacterium ulcerans strain, Germany, 2012. Emerging Infectious Diseases 21(2), 356.

Mikhailovich VM, Melnikov VG, Mazurova IK, Wachsmuth IK, Wenger JD, Wharton M, Nakao H, Popovic T. 1995. Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994. Journal of Clinical Microbiology 33(11), 3061-3063.

Morris AJ, Frohman MA, Engebrecht J. 1997. Measurement of phospholipase D activity. Analytical Biochemistry 252(1), 1-9.

Moussa IM, Ali MS, Hessain AM, Kabli SA, Hemeg HA, Selim SA. 2016. Vaccination against Corynebacterium pseudotuberculosis infections controlling caseous lymphadenitis (CLA) and oedematousskin disease. Saudi Journal of Biological Sciences 23(6), 718-723.

Nielsen BP, Scherling B, Scheibel, JH, Frederiksen W. 1991. Diphtheria in Denmark 1956-1989. Occurence of Corynebacterium diphtheirae and other diphtheria toxigenic bacteria Ugeskrift for Laeger 153(11), 769-772.

NIH. 2015. Field Epidemiology & Disease Surveillance Division. Pakistan: National Institute of Health.

Paton M, Sutherland S, Rose I, Hart R, Mercy A, Ellis T. 1995. The spread of Corynebacterium pseudotuberculosis infection to unvaccinated and vaccinated sheep. Australian Veterinary Journal 72(7), 266-269.

Paton M, Walker S, Rose I, Watt G. 2003. Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccines in sheep flocks. Australian veterinary journal 81(12), 91-95.

Peel MM, Palmer GG, Stacpoole AM, Kerr TG. 1997. Human lymphadenitis due to Corynebacterium pseudotuberculosis: report of ten cases from Australia and review. Clinical Infectious Diseases 24(2), 185-191.

Piontkowski M, Shivvers D. 1998. Evaluation of a commercially available vaccine against Corynebacterium pseudotuberculosis for use in sheep. Journal of the American Veterinary Medical Association 212(11), 1765-1768.

Ren J, Kachel K, Kim H, Malenbaum SE, John R, London E. 1999. Interaction of diphtheria toxin T domain with molten globule-like proteins and its implications for translocation. Science 284(5416), 955-957.

Riegel P, Ruimy R, De Briel D, Prevost G, Jehl F, Christen R, Monteil H. 1995. Taxonomy of Corynebacterium diphtheriae and related taxa, with recognition of Corynebacterium ulcerans sp. nov. nom. rev. FEMS Microbiology Letters 126(3), 271-276.

Rodnin MV, Li J, Gross ML, Ladokhin AS. 2017. The pH-Dependent Trigger in Diphtheria Toxin T Domain Comes with a Safety Latch. Biophysical Journal 112(3), 184a.

Sadoh A, Sadoh W. 2011. Diphtheria mortality in Nigeria: the need to stock diphtheria antitoxin. African Journal of Clinical and Experimental Microbiology 12(2).

Sangal V, Hoskisson PA. 2016. Evolution, epidemiology and diversity of Corynebacterium diphtheriae: new perspectives on an old foe. Infection, Genetics and Evolution 43, 364-370.

Sekizuka T, Yamamoto A, Komiya T, Kenri T, Takeuchi F, Shibayama K, Takahashi M, Kuroda M, Iwaki M. 2012. Corynebacterium ulcerans 0102 carries the gene encoding diphtheria toxin on a prophage different from the C. diphtheriae NCTC 13129 prophage. BMC Microbiology 12(1), 72.

Selim S. 2001a. Oedematous skin disease of buffalo in Egypt. Zoonoses and Public Health 48(4), 241-258.

Selim S. 2001b. Oedematous skin disease of buffalo in Egypt. Journal of Veterinary Medicine, Series B 48(4), 241-258.

Selim SA, Mohamed FH, Hessain AM, moussa IM. 2016. Immunological characterization of diphtheria toxin recovered from Corynebacterium pseudotuberculosis. Saudi Journal of Biological Sciences 23, 282-287.

Seto Y, Komiya T, Iwaki M, Kohda T, Mukamoto M, Takahashi M, Kozaki S. 2008. Properties of corynephage attachment site and molecular epidemiology of Corynebacterium ulcerans isolated from humans and animals in Japan. Japanese Journal of Infectious Diseases 61(2), 116.

Shapira A, Benhar I. 2010. Toxin-based therapeutic approaches. Toxins 2(11), 2519-2583.

Sharma J, Malakar M, Gupta S. 2013. First evidence of diphtheria cases in Dhubri district of Assam, India. India Streams Research Journal 3(11).

Singh N, Singh AK, Gaur S. 2013. A study of diphtheria menace in Kumaun region of Uttarakhand state in India. Journal of Drug Delivery and Therapeutics 3(4), 105-107.

Soares SC, Silva A, Trost E, Blom J, Ramos R, Carneiro A, Ali A, Santos AR, Pinto AC, Diniz C. 2013. The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains. PloS One 8(1), e53818.

Srivastava S, Luqman S. 2015. Immune-O-Toxins as the magic bullet for therapeutic purposes. Biomedical Research and Therapy 2(1), 169-183.

Stanford K, Brogden K, McClelland L, Kozub G, Audibert F. 1998. The incidence of caseous lymphadenitis in Alberta sheep and assessment of impact by vaccination with commercial and experimental vaccines. Canadian Journal of Veterinary Research 62(1), 38.

Tao X, Zeng HY, Murphy JR. 1995. Transition metal ion activation of DNA binding by the diphtheria tox repressor requires the formation of stable homodimers. Proceedings of the National Academy of Sciences 92(15), 6803-6807.

Taylor J, Saavedra-Campos M, Harwood D, Pritchard G, Raphaely N, Kapadia S, Efstratiou A, White J, Balasegaram S. 2010. Toxigenic Corynebacterium ulcerans infection in a veterinary student in London, United Kingdom, May 2010. Eurosurveillance 15(31), 19634.

Tiwari TS, Golaz A, Yu DT, Ehresmann KR, Jones TF, Hill HE, Cassiday PK, Pawloski LC, Moran JS, Popovic T. 2008. Investigations of 2 cases of diphtheria-like illness due to toxigenic Corynebacterium ulcerans. Clinical Infectious Diseases 46(3), 395-401.

TonThat H, Marraffini LA, Schneewind O. 2004. Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae. Molecular Microbiology 53(1), 251-261.

TonThat H, Schneewind O. 2003. Assembly of pili on the surface of Corynebacterium diphtheriae. Molecular Microbiology 50(4), 1429-1438.

Torres Lde F, Ribeiro D, Hirata R, Jr., Pacheco LG, Souza MC, dos Santos LS, dos Santos CS, Salah M, Costa MM, Ribeiro MG, Selim SA, Azevedo VA, Mattos-Guaraldi AL. 2013. Multiplex polymerase chain reaction to identify and determine the toxigenicity of Corynebacterium spp with zoonotic potential and an overview of human and animal infections. Memorias do Instituto Oswaldo Cruz 108(3). doi: 10.1590/s0074-02762013000300003

Trost E, Al-Dilaimi A, Papavasiliou P, Schneider J, Viehoever P, Burkovski A, Soares SC, Almeida SS, Dorella FA, Miyoshi A. 2011. Comparative analysis of two complete Corynebacterium ulcerans genomes and detection of candidate virulence factors. BMC Genomics 12(1), 383.

von Hunolstein C, Alfarone G, Scopetti F, Pataracchia M, La Valle R, Franchi F, Pacciani L, Manera A, Giammanco A, Farinelli S. 2003. Molecular epidemiology and characteristics of Corynebacterium diphtheriae and Corynebacterium ulcerans strains isolated in Italy during the 1990s. Journal of Medical Microbiology 52(2), 181-188.

Wagner K, White J, Crowcroft N, De Martin S, Mann G, Efstratiou A. 2010. Diphtheria in the United Kingdom, 1986–2008: the increasing role of Corynebacterium ulcerans. Epidemiology and Infection 138(11), 1519-1530.

Wellinghausen N, Sing A, Kern WV, Perner S, Marre R, Rentschler J. 2002. A fatal case of necrotizing sinusitis due to toxigenic Corynebacterium ulcerans. International Journal of Medical Microbiology 292(1), 59-63.

White A, Ding X, Murphy JR, Ringe D. 1998. Structure of the metal-ion-activated diphtheria toxin repressor/tox operator complex. Nature 394(6692), 502.

Williamson LH. 2001. Caseous lymphadenitis in small ruminants. Veterinary Clinics: Food Animal Practice 17(2), 359-371.

Wong TP, Groman N. 1984. Production of diphtheria toxin by selected isolates of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. Infection and Immunity 43(3), 1114-1116.

Yanagawa R, Honda E. 1976. Presence of pili in species of human and animal parasites and pathogens of the genusCorynebacterium. Infection and Immunity 13(4), 1293-1295.

Yasuda I, Matsuyama H, Ishifuji T, Yamashita Y, Takaki M, Morimoto K, Sekino M, Yanagihara K, Fujii T, Iwaki M. 2018. Severe Pneumonia Caused by Toxigenic Corynebacterium ulcerans Infection, Japan. Emerging Infectious Diseases 24(3), 588.

Yeruham I, Braverman Y, Shpigel N, ChizovGinzburg A, Saran A, Winkler M. 1996. Mastitis in Dairy Cattle Caused by Corynebacterium pseudotuberculosis and the Feasibility Of Transmission by Houseflies I. Veterinary Quarterly 18(3), 87-89.

Yeruham I, Friedman S, Perl S, Elad D, Berkovich Y, Kalgard Y. 2004. A herd level analysis of a Corynebacterium pseudotuberculosis outbreak in a dairy cattle herd. Veterinary Dermatology 15(5), 315-320.

Rehman Ali, Marina Khan, Muhammad Rooman.
Toxigenic diphtheria: A review of the current knowledge.
Int. J. Biosci. 16(3), 518-535, March 2020.
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