Immunohistochemical markers of mammary tumors in female dogs from Northeastern Algeria
Paper Details
Immunohistochemical markers of mammary tumors in female dogs from Northeastern Algeria
Abstract
The present study was undertaken to investigate the importance of immunohistochemical markers (cytokeratins AE1/AE2, CK20, CK5, 6, RE, vimentin and P63) in tumor type diagnosis in the case of canine mammary tumors. Thirteen (13) tumors tissue specimens were obtained from 42 female dogs in different ages and breeds. They had been classified according to WHO method after histopathological examination. The tumors were diagnosed as Squamous cell carcinoma (3 cases), Spindle cell carcinomas (2 cases), Sarcomas (3 cases), Carcinoma simple cribriform (2 cases) and the epithelial component is malignant, and the myoepithelium is benign (3 cases). Cytokeratins AE1/AE2 were seen in all cases except in the spindle cell carcinoma. Vimentin can be used as a myoepithelial and mesenchymal cell marker in all cases except in the malin myoepithelioma, P63 is a sensitive and specific myoepithelial marker in canine mammary tumors. Whereas CK 5, 6 and RE were specifically detected in simple cribriform Carcinoma, cytokeratins CK20 were expressed in all cases except in the Squamous cell carcinoma. These results emphasize the interest of immunohistochemical markers to identify the humoral cell origin in canine mammary tumors.
Aydogan A, Metin N. 2013. Detection of cell origin by immunohistochemistry in canine mammary tumours. Revue de Médecine Vétérinaire 164(7), 395-399.
Bonnie LK. 2002. Immunohistochemical analysis of breast cells obtained by ductal lavage. Cancer Cytopatholy96, 244-249. http://dx.doi.org/10.1002/cncr.10719
Bussolati G, Gugliotta P, Morra I, Pietribiasi F, Berardengo E. 1986. The immunohistochemical detection of lymph node metastases from infiltrating lobular carcinoma of the breast.British Journal of Cancer 54, 631-63.
Destexhe E, Lespagnard L, Degeyter M, Heymann R, Coignoul F. 1993. Immunohistochemical identification of myoepithelial, epithelial and connective tissue cells in canine mammary tumors. Veterinary Pathology 30, 146-154. http://dx.doi.org/10.1177/030098589303000207
Gama A, Alves A, Gartner F, Schmitt F. 2003. p63: A novel myoepithelial cell markers in canine mammary tissues. Veterinary Pathology 40, 412-420. http://dx.doi.org/10.1354/vp.40-4-412
Gärtner F, Geraldes M, Cassali G. 1999. DNA measurement and immunohistochemical characterization of epithelial and mesenchymal cells in canine mixed mammary tumors: Putative evidence for a common histogenesis. Veterinary Journal 158, 39-47. http://dx.doi.org/10.1053/tvjl.1998.0333
Griffey SM, Madewell BR, Dairkee SH, Hunt JE, Naydan DK, Higgins RJ. 1993. Immunohistochemical reactivity of basal and luminal epithelium-specific cytokeratin antibodies within normal and neoplastic canine mammary glands. Veterinary Pathology 30, 155-161. http://dx.doi.org/10.1177/030098589303000208
Karayannopoulou M, Kaldrymidou E, Constantinidis TC, Dessiris A. 2005. Histological grading and prognosis in dogs with mammary carcinomas: Application of a human grading method. Journal of Comparative Pathology, 133, 246–252. http://dx.doi.org/0.1016/j.jcpa.2005.05.003
Karayannopoulou M, Lafioniatis S. 2016.Recent advances on canine mammary cancer chemotherapy: A review of studies from 2000 to date. Revue de MédecineVétérinaire167(7-8), 192-200.
Mallofré C, Castillo M, Morente V, Solé M. 2003. Immunohistochemical expression of CK20, p53, and Ki-67 as objective markers of urothelial dysplasia. Modern Pathology 16(3), 187–191. http://dx.doi.org/01.MP.0000056628.38714
Dias ML de M, Joanlise M, Leon Andrade, Márcio B. de Castro, Paula D. Galera. 2016. Survival analysis of female dogs with mammary tumors after mastectomy: epidemiological, clinical and morphological aspects. Pesquisa Veterinária Brasileira 36(3),181-186. http://dx.doi.org/10.1590/S0100736X2016000300006
Morrison WB. 1998. Canine and feline mammary tumors. In: Cancer in Dogs and Cats: Medical and Surgical Management, WB. Morrison, Ed., Williams and Wilkins, Baltimore, Maryland, pp. 591–598.
Perez A, Pena L, Del Castillo N, Nieto AI. 2000. Factors influencing the incidence and prognosis of canine mammary tumors. Journal of Small Animal Practice 41, 287–291. http://dx.doi.org/10.1111/j.17485827.2000.tb03203.x
Rao NAS, Van Wolferen ME, Gracanin A, Bhatti SFM, Krol M, Holstege FC, Mol JA. 2009. Gene expression profiles of progestin-induced canine mammary hyperplasia and spontaneous mammary tumors. Journal of Physiology and Pharmacology Suppl 1, 73–84.
Toniti P, Sirivisoot S, Jandee P, Srimontri P, Puchadapirom P, Doungchawee G, Kasorndorkbua C. 2010. AE1/AE3, Vimentin and p63 Immunolocalization in canine mammary gland tumours: roles in differentiation between luminal epithelial and myoepithelial lineages. Asian Pacific Journal of Cancer Prevention 11, 227-230.
Van Houdt L, Hellmén E. 2005. Immunohistochemical study of canine mammary gland tumors. Uppsala University Rudbeck Laboratory Department of Genetics and Pathology, Research Project, 29-55 P.
Vos J.H, Van DenIngh TS, Misdorp W, Molenbeek RF, Van Mil FN, Rutterman GR, Ivanyi D, Ramaekers FCS. 1993. Immunohistochemistry with keratin, vimentin, desmin, and α-smooth muscle actin monoclonal antibodies in canine mammary gland: malignant mammary tumors. The Veterinary Quarterly 14, 96-102. http://dx.doi.org/01652176.1993.9694382
Yaziji H, Gown AM, Sneige N. 2000. Detection of stromal invasion in breast cancer: the myoepithelial markers. Advances in Anatomic Pathology 7, 100-109. http://dx.doi.org/10.1097/00125480-200007020-0000
Zahra Gabli, Leila Beddar, Zouhir Djerrou (2018), Immunohistochemical markers of mammary tumors in female dogs from Northeastern Algeria; IJB, V12, N4, April, P330-335
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