home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Investigating the effects of drought stress on photosynthetic electron transport chain of two basil (Ocimum basilicum L.) cultivars by measuring ‘Chlorophyll-a’ fluorescence

Paper Details

Research Paper 01/07/2015
Views (331) Download (21)
current_issue_feature_image
publication_file

Investigating the effects of drought stress on photosynthetic electron transport chain of two basil (Ocimum basilicum L.) cultivars by measuring ‘Chlorophyll-a’ fluorescence

Shima Motamed, Mojtaba Jafarinia, Bahman Kholdebarin
J. Bio. Env. Sci.7( 1), 564-571, July 2015.
Certificate: JBES 2015 [Generate Certificate]
Key: Chlorophyll-aDrought stressFluorescenceGreen basilPurple basil

Abstract

Drought stress is one of the most important abiotic stresses that affects plant growth and development. In recent years, due to low precipitation and drought conditions, studying the effects of low water stress on plants performance have gained significance. Basil (Ocimum basilicum L.) belongs to Lamiaceae family which has many medicinal properties and is also being used as fresh edible vegetable. Two basil cultivars (green and purple) were grown in pots and were subjected to two water regimes of 100% (control) and 25% (drought stress) field capacity respectively. Handy PEA instrument was used to measure leaves chlorophyll fluorescence. Results showed that under drought stress the photosynthetic electron transport efficiency of green and purple basil cultivars were reduced by 57.73 and 47.11% respectively, as compared with their controls. Investigating the chlorophyll-a fluorescence also revealed that the activities of water photolysis complex, electron transport to QA acceptor and also the electron transport from the mid electron transport chain to photo system I were reduced under drought stress. With respect to electron transport efficiency in the middle of electron transport chain from b6f complex to photo system I, purple basil performed better than green basil and as a result had higher tolerance to drought stress.

VIEWS 18
Cover PDF

Ait AN, Dewes D, Didur O. 2006. Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex. Photosynthesis Research 89, 81-87.

Baker NR, Rosenqvist E. 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany 55, 607-1621.

Chen LS, Cheng L. 2010. The acceptor side of photosystem II is damaged more severely than the donor side of photosystem II in Honey crisp apple leaves with zonal chlorosis. Acta Physiological Plantarum 32, 253-26.

Cheong YH, Kim KN, Pandey GK, Gupta R, Grant JJ, Luan S. 2003. CBL, a calcium sensor that differentially regulates salt drought and cold responses in Arabidopsis. The Plant Cell 15 ,1833-1845.

Delkhosh B, Shirani Rad AH, Noor Mohamadi G. 2006. Effect of drought stress on yield and chlorophyll content of canola. Journal of Agricultural Science 12(2), 359-367.

Imam Y, Zavarehi M. 2005. Drought Tolerance in Higher Plants (Genetically, Physiological and Molecular Biological Analysis). Academic Publishing Center of Tehran 168, 1016-1025.

Jafarinia M, Shariati M. 2012. Effect of salt stress on photosystem II of canola (Brassica napus, L.) plant probing by chlorophyll a fluorescence measurements. Iranian Journal of science and Technology 1, 71-76.

Javanmardi J, Khalighi A, Kashi A, Bais HP, Vivanco JM. 2002.  Chemical  characterization  of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. Journal of Agricultural Food Chemistry 50, 5878-5883.

Kocheva K, Lambrev P, Georgiev G, Goltsev V. 2004. Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress. Bio Electro Chemistry 18(63), 121-124.

Korkmaz A, Uzunlu M, Demirkiran AR. 2007. Treatment with acetylsalicylic acid protects muskmelon seedlings against drought stress. Acta Physiological Plant 29, 503-508.

Lazar D. 2009. Modeling of light-induced chlorophyll a fluorescence rise (O-J-I-P transient) and changes in 820 nm-transmittance signal of photosynthesis. Photosynthetica 47, 483-498.

Lu C, Zhang J. 1999. Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. Journal of Experimental Botany 50, 1199-1206.

Mark CFR, Redillas R, Strasser J. 2011. The use of JIP test to evaluated drought tolerance of transgenic rice overexpressing. Korean society for plant biotechnology and Springe 5, 169-175.

Mehta P, Jajoo A, Mathur S, Bhrati S. 2010. Chlorophyll a fluorescence study revealing effects of high salt stress on photosystem II in wheat leaves. Plant Physiology and Biochemistry 48, 16-20.

Oukarroum A, Madidi S, Schansker E, Strasser RJ. 2007. Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. Environmental and Experimental Botany 60(3), 438-446.

Simon JE, Bubenheim RD, Jolly RJ, Charles DJ. 1992. Water stress induced alternations inessential oil content and composition of sweet basil. Journal of Essential Oil Research 4, 71-75.

Strasser RJ, Srivastava A, Tsimilli-Michael M. 2004. Analysis of the chlorophyll a fluorescence transient. In: Chlorophyll a fluorescence: A signature of photosynthesis. (eds. Papagrorgiou, G. C. and Govindjee ). Springer, Rotterdam 321-362.

Thach LB, Shapcott A, Schmidt S. 2007. The OJIP fast fluorescence rise characterizes Graptophyllum species and their stress responses. Photosynthetic Research 94, 423-436.

Wang GP, Zhang J, Zhao MR, Hui Z, Wang W. 2010. Overaccumulation of glycine betaine enhance tolerance of the photosynthetic apparatus to drought and heat stress in wheat. Photosynthetica 48, 30-41.

Xia J, Li Y, Zou D. 2004. Effects of salinity stress on PSII in Ulva lactuca as probed by chlorophyll fluorescence measurements. Aquatic Botany 80, 129-137.

Submit Manuscript