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Investigation of genetic diversityin Pakistan I Brassica Compestris Landraces for the selection of high yielding genotypes

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Research Paper 01/10/2018
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Investigation of genetic diversityin Pakistan I Brassica Compestris Landraces for the selection of high yielding genotypes

Badshah Hussain, S.F. Wadood, Noor Muhammad, Niaz Ali, Hayat Ullah, Nisar Uddin, Ahmad Sher, Mohammad Nisar
Int. J. Biosci.13( 4), 183-194, October 2018.
Certificate: IJB 2018 [Generate Certificate]
Key: Brassica CompestrisGenetic diversityMorphologySDS-PAGE

Abstract

Brassica compestris vegetables are of great economic importance throughout the world and are utilized for Brassica oil seed. Its production has increased over the last 40 years and has become one of the most important world sources of vegetable oil after soya bean and cotton seed. Genetic diversity is important in selecting high yielding genotypes. For this purpose 50 genotypes of Brassica campestris seeds were collected from different region of Dir lower Khyber Pukhtun Khwa, Pakistan. These genotypes were grown in Botanical Gardan Department of Botany, University of Malak and Khyber Pukhtun Khwa, Pakistan, for the estimation of genetic diversity using the morphological traits and Biochemical marker. A total of 18 morphological parameters were scored for estimation of genetic diversity and phylogenetic relationship through cluster plotting. Similarly, six loci (bands) were detected in the collected genotypes, out of them locus-1 (band-1) contain 100% protein bands and marked as monomorphic locus. The remaining loci i.e. 2,3,4,5 and 6 were polymorphic and show 28%, 42%, 36%, 56% and 25% variation respectively. Inter specific locus contribution toward genetic diversity (LCTGD) was 83.33% in the collected genotypes. SDS-PAGE profiling based on Two-way cluster plotting successfully resolved the collected genotypes into 4 clusters. The present result shows that SDS PAGE is a powerful technique for the estimation of intra-specific Genetic diversity.

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