Moderate halophilic bacterial community in excreta of wild ass (Equus hemionus khur)
Paper Details
Moderate halophilic bacterial community in excreta of wild ass (Equus hemionus khur)
Abstract
Twenty-four moderate halophiles were isolated from excreta of wild ass, designated as Mk-1 to Mk-24. All the organisms were Gram’s positive and non-capsulated. Biochemical characterization was performed on the basis of Bergey’s Manual of Systematic Bacteriology and all the isolates were belongs to Bacillus genera. Organisms were able to grow optimally at pH 5-6, 30-40˚C temperature and 10-15% NaCl (w/v) while no growth below 5% NaCl indicated moderate halophilic nature of isolates. All were grown in the presence of bile salt, indicated intestinal origin. Organisms were further explored for extracellular hydrolytic enzyme production such as lipases, proteases, amylases, cellulases and chitinase, apart from first aim to focus on biodiversity studies.
Amoozegar MA, Malekzadeh F, Malik KA. 2003. Production of Amylase by newly isolated moderate halophile, Halobacillus sp. Strain MA-2. Journal of Microbiological Methods 52, 353-359.
Amoozegar MA, Salehghamari E, Khajeh K, Kabiri M, Naddaf S. 2008. Production of an extracellular thermohalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2. Journal of Basic Microbiology 48, 160– 167.
De Lourdes MM, García MT, Ventosa A, Mellado E. 2009. Characterization of Salicola sp. IC10, a lipase- and protease-producing extreme halophile. FEMS Microbiology Ecology 68, 59–71.
Galinski EA. 1993. Compatible solutes of halophilic eubacteria; molecular principles, water-solute interaction, stress protection. Experientia 49, 487-495.
Govender L, Naidoo L, Setati ME. 2009. Isolation of hydrolase producing bacteria from Sua pan solar salterns and the production of endo-1,4- β-xylanase from a newly isolated haloalkaliphilic Nesterenkonia sp. African Journal of Biotechnology 8, 5458- 5466.
Hashim SO. 2004. An alkaline active maltooligosaccharide forming β-amylase from Bacillus halodurans. Ph.D. Thesis, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University Sweden.
Kamekura M, Hamakawa T, Onishi H. 1982. Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5’-GMP. Applied and Environmental Microbiology 44, 994–995.
Kamekura M, Onishi H. 1978. Properties of the halophilic nuclease of a moderate halophile, Micrococcus varians subsp. halophilus. Journal of Bacteriology 133, 59-65.
Menon, V. 2003. A Field Guide to Indian Mammals. Dorling Kindersley India Pvt. Ltd., 199-220.
Moehlman, Patricia D. 2002. Equids: Zebras, Asses and Horses. Status Survey and Conservation Action Plan. IUCN/SSC Equid Specialist Group. IUCN, Gland, Switzerland and Cambridge, UK. 190-199.
Muntyan MS, Tourova TP, Lysenko AM, Kolganova TV, Fritze D, Skulachev VP. 2002. Molecular identification of alkaliphilic and halotolerant strain Bacillus sp. FTU as Bacillus pseudofirmus FTU”. Extremophiles 6, 195- 199.
Nieto JJ, Vargas C. 2002. Synthesis of osmoprotectants by moderately halophilic bacteria: genetic and applied aspects. Recent Development in Microbiology 6, 403–418.
Onishi H. 1972. Halophilic amylase from moderately halophilic Micrococcus. Journal of Bacteriololy 109, 570-574.
Prakash S, Veeranagouga Y, Kyoung L, Sreeramulu K. 2009. Xylanase production using inexpensive agricultural wastes and its partial characterization from a halophilic Chromohalobacter sp. TPSV 101. Process Biochemistry 25, 197-204.
Rohban R, Amoozegar MA, Ventosa A. 2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. Indian Journal of Microbiology and Biotechnology 36, 333-340.
Romano I, Giordano A, Lama L, Nicolaus B, Gambacorta A. 2005. Halomonas campaniensis sp. nov. a haloalkaliphilic bacterium isolated from a mineral pool of Campania Region, Italy. Systematic and Applied Microbiology 28, 610-618.
Romano I, Nicolaus B, Lama L, Manca MC, Gambacorta A. 1996. Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria Island. Systematic and Applied Microbiology 19, 326-333.
Sanchez-Porro C, Martin S, Mellado E, Ventosa A. 2003. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of Applied Microbiology 94, 295–300.
Ventosa A, Nieto JJ, Oren A. 1998. Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular biology Review 62, 504–544.
Ventosa A, Nieto JJ. 1995. Biotechnological applications and potentialities of halophilic microorganisms. World Journal of Microbiology and Biotechnology 11, 85-94.
Vidyasagar M, Prakash S, Mahajan V, Shouche YS, Sreeramulu K. 2009. Purification and characterization of an extreme halothermophilic protease from a halophilic bacterium Chromohalobacter sp. TVSP101. Brazilian Journal of Microbiology 40, 12-19.
Mrugesh Khunt, Neepa Pandhi, 2011. Moderate halophilic bacterial community in excreta of wild ass (Equus hemionus khur). Int. J. Biosci., 1(5), 31-37.
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