Toxicity of crude extracts from soft corals (Anthozoa, Alcyonacea) collected at varying wave exposure sites in Talisayan, Northern Mindanao, Philippines
Paper Details
Toxicity of crude extracts from soft corals (Anthozoa, Alcyonacea) collected at varying wave exposure sites in Talisayan, Northern Mindanao, Philippines
Abstract
Octocorallian soft corals are known for their ecological and pharmacological importance, but the bioactivity of crude extracts from Philippine species is less studied. This study determined the toxicity of crude extracts from species collected at wave-exposed and less wave-exposed nearshore sites at Talisayan, Southern Philippines. SCUBA diving was used to collect soft coral specimens at 3-5 m depth. In situ colony image and sclerites from each specimen were used to identify soft corals to the species level. The brine shrimp lethality assay was carried out to determine the toxicity of methanol extracts at 10, 100 and 1000 ppm concentrations. This study recorded three species (Sarcophyton crassocaule, Isis hippuris, Cespitularia stolonifera) from the less wave-exposed site, and eight from the wave-exposed area (Sinularia sp., Si. flexibilis, Lobophytum crassum, and L. durum, Si. polydactyla, S. ehrenbergi, S. glaucum, and Dendronephthya hemprichii). Toxicity of extracts against brine shrimps appears not to be due to the wave exposure of site, but more associated with the species and perhaps intensity of fish predation on soft corals. Extracts from S. crassocaule, L. durum, Isis hippuris, L. crassum, S. ehrenbergi, C. stolonifera, and D. hemprichii were highly toxic with LC50 values (in ppm) of 0.40, 3.45, 3.67, 4.25, 4.25, 26.92, and 28.18, respectively. Si. flexibilis was considered toxic with an LC50 value of 144.54 ppm. The unidentified species Sinularia sp. was found to be non-toxic. This study reveals that some soft coral taxa from Talisayan neritic waters are potentially good sources of bioactive compounds for drug discovery.
Abdel-Wahhab MA, El-Nekeety AA, Hassan NS, El-Hefnawy, Kotb MM, El-Mekkawy, Khalil NA, Hanna AG. 2012. Hepatoprotective effect of sarcophine isolated from soft coral (Sarcophyton glaucum) in rats. Global Veterinaria 8(3), 244–253.
Bakus GJ. 1981. Chemical defense mechanisms on the Great Barrier Reef, Australia. Science 211(4481), 497–499.
Chou TZ, Liang CH, Wang GH, Liaw CC, Lee MF, Wang SH, Cheng DL. 2008. Extracts from Cladiella australis, Clavularia viridis and Klyxum simplex (soft corals) are capable of inhibiting the growth of human oral squamous cell carcinoma cells. Marine Drugs 6, 595–606. http://dx.doi.org/10.3390/md6040595
Chao CH, Wen ZH, Chen IM, Su JH, Huang HC, Chiang MY, Sheu JH. 2008. Anti-inflammatory steroids from the octocoral Dendronephthya griffin. Tetrahedron 64, 3554–60. http://dx.doi.org/10.1016/j.tet.2008.01.109
Coll JC, Bowden BF, Mitchell SJ. 1980. Marine natural products chemistry at the James Cook University of North Queensland. Chemistry Australia 47, 259–263.
Coll JC, La Barrel S, Sammarco PW, Williams WT, Bakus GJ. 1983. Chemical defences in soft corals (Coelenterata: Octocorallia) of the Great Barrier Reef: a study of comparative toxicities. Marine Ecology Progress Series 8, 271–278.
Duh CY, Puu SY, Wang SK. 2012. New 19-oxygenated steroids from the soft coral Nephthea chabrolii. Marine Drugs 10, 1288–1296. http://dx.doi.org/10.3390/md10061288
Edrada RU, Proksch P, Wray V, Witte L, van Ofwegen L. 1998. Four new bioactive lobane diterpenes of the soft coral Lobophytum pauciflorum from Mindoro, Philippines. Journal of Natural Products 61, 358–361. http://dx.doi.org/10.1021/np970276t
El-Gamal AAH, Chiang CY, Huang SH, Wang SK, Duh CY. 2005. Xenia diterpenoids from the formosan soft coral Xenia blumi. Journal of Natural Products 68, 1336−1340. http://dx.doi.org/10.1021/np058047r
Fabricius K, Alderslade P. 2001. Soft corals and sea fans: A comprehensive guide to the tropical shallow water genera of the central-west Pacific, the Indian Ocean and the Red Sea. Australian Institute of Marine Science, and the Museum and Art Gallery of the Northern Territory.
Fabricius K, Déath G. 2001. Biodiversity on the Great Barrier Reef: Large-scale patterns and turbidity related local loss of soft coral taxa. Oceanographic Processes of Coral Reefs. Retrieved October 19, 2017 from epubs.aims.gov
Goh BL, Tan GE, Tan LT. 2009. Diversity, distribution and biological activity of soft corals (Octocorallia, Alcyonacea) in Singapore. Journal of Coastal Development 12(2), 89–98.
Hirono M, Ojika M, Mimura H, Nakanishi Y, Maeshima M. 2003. Acylspermidine derivatives isolated from a soft coral, Sinularia sp., inhibit plant vacuolar H+-Pyrophosphatase. Journal of Biochemistry 133, 811–816. http://dx.doi.org/10.1093/jb/mvg103
Janes MP. 2008. Advances in coral husbandry in public aquariums. Public Aquarium Husbandry Series 2, 413–426.
Johnson JE, Marshall PA. 2007. Climate Change and the Great Barrier Reef. Great Barrier Marine Park Authority and Australian Greenhouse Office, Australia.
Kelman D, Benayahu Y, Kashman Y. 1999. Chemical defence of the soft coral Parerythropodium fulvum (Forskål) in the Red Sea against generalist reef fish. Journal of Experimental Marine Biology and Ecology 238, 127−137. http://dx.doi.org/10.1016/S0022-0981(99)00016-7
König GM, Coll JC, Bowden BF, Gulbis JM, MacKay MF, La Barre SC, Laurent D. 1989. The structure determination of a xenicane diterpene from Xenia garciae. Journal of Natural Products 52, 294−299. http://dx.doi.org/10.1021/np50062a013
Li G, Deng Z, Guan H, van Ofwegen L, Proksch P, Lin W. 2005. Steroids from the soft coral Dendronephthya sp.. Steroids 70(1), 13–8. http://dx.doi.org/10.1016/j.steroids.2004.09.003
Limna Mol VP, Raveendran TV, Parameswaran PS, Kunnath RJ, Sathyan N. 2010. Antifouling sesquiterpene from Indian soft coral, Sinularia karavattiensis Aldersalde and Prita. Indian Journal of Marine Sciences 39(2), 270–273.
Luyao HN, Metillo EB, Uy MM. 2019. Evaluation of potential cytotoxicity of the extracts from soft corals collected from the Philippine Sea of Mindanao: Sarcophyton glaucum, Lobophytum pauciflorum, Sinularia flexibilis and Lobophytum crassum. International Journal of Biosciences 14(2), 439–445. http://dx.doi.org/10.12692/ijb/14.2.439-445
Maida M, Sammarco PW, Coll JC. 1995. Effects of soft corals on scleractinian coral recruitment. 1. Directional allelopathy and inhibition of settlement. Marine Ecology Progress Series 121, 191–202. http://dx.doi.org/10.3354/meps121191
Maida M, Sammarco PW, Coll JC. 2001. Effects of soft corals on scleractinian coral recruitment. II: allelopathy, spat survivorship and reef community structure. Marine Ecology 22(4), 397–414. https://doi.org/10.1046/j.1439-0485.2001.01709.x
Maida M, Sammarco PW, Coll JC. 2006. A diffusion chamber for assessing efficacy of natural anti-fouling defenses in marine organisms. Journal of Experimental Marine Biology and Ecology 337(1), 59–64. http://dx.doi.org/10.1016/j.jembe.2006.06.008
Maiorana VC. 1979. Nontoxic toxins: the energetics of coevolution. Biological Journal of the Linnean Society 11(4), 387–396.
Mehdinia A, Fumani S, Rezaei H. 2014. Essential oils of a soft coral (Sinularia sp.) from Chabahar Bay of Iran. Journal of the Persian Gulf 5(15), 51–58.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine shrimp: A convenient general bioassay for active plant constituents. Journal of Medicinal Plant Research 45, 31–34. http://dx.doi.org/10.1055/s-2007-971236
Murcia SRS, Metillo EB, Uy MM. 2019. The brine shrimp toxicity of the philippine marine sponges Axinyssa sp., Plocamionida sp., Forcepia sp., Pachymatisma sp., and Placospongia sp.. International Journal of Biosciences 14(3), 30–34. http://dx.doi.org/10.12692/ijb/14.3.30-34
Nakamura M, Ohki S, Suzuki A, Sakai K. 2011. Coral larvae under ocean acidification: survival, metabolism, and metamorphosis. PLoS ONE, 6, e14521. http://dx.doi.org/10.1371/journal.pone.0014521
Phan CS, Vairappan CS. 2015. Isopropyl (ene)-type cembrane diterpene an important chemotaxonomical marker in Bornean soft coral genus Sarcophyton. Journal of Tropical Biology and Conservation 12, 137–143.
Putra MY, Murniasih T. 2016. Marine soft corals as source of lead compounds for anti- inflammatories. Journal of Coastal Life Medicine 4(1), 73–77. http://dx.doi.org/10.12980/jclm.4.2016j5-226.
Reaka-Kudla M. 1997. The global biodiversity of coral reefs: a comparison with rain forests. In Biodiversity II: Understanding and Protecting our Biological Resources. The National Academy of Sciences, USA.
Sammarco PW, La Barre S, Coll JC. 1987. Defensive strategies of soft corals (Coelenterata: Octocorallia) of the Great Barrier Reef III. The relationship between ichthyotoxicity and morphology. Oecologia 74, 93–101. http://dx.doi.org/10.1007/BF00377351
Sammarco PW, Coll JC. 1988. The chemical ecology of alcyonarian corals (Coelenterata: Octocorallia). In: Scheuer, P.J. (Ed.), Bioorganic marine chemistry, Springer-Verlag, Berlin p, 87–116.
Sayuti M, Putri WDR, Yunianta. 2016. Phytochemicals screening and antioxidant activity test of Isis hippuris methanol extract. International Journal of ChemTech Research 9(7), 427–434.
Shahbudin S, Deny, Zakirun AMT, Haziyamin TAH, Akbar JB, Taher M. 2011. Antioxidant properties of soft coral Dendronephthya sp. International Journal of Pharmacology 7(2), 263– 267. http://dx.doi.org/10.3923/ijp.2011.263.267
Sheu JH, Hung KC, Wang GH, Duh CY. 2000. New cytotoxic sesquiterpenes from the gorgonian Isis hippuris. Journal Natural Products 63(12), 1603–1607. http://dx.doi.org/10.1021/np000271n
Sheu Jh, Lin WY, Lu Y, Su JH, Wen ZH, Dai CF, Kuo YF. 2011. Bioactive cembranoids from the Dongsha Atoll soft coral Sarcophyton crassocaule. Marine Drugs 9, 994–1006. http://dx.doi.org/10.3390/md9060994
SPSS. 2016. IBM SPSS Statistics 24. IBM Corporation, NY, USA.
Sung PJ, Chen KH, Dai CF, Lu MC, Li JJ, Chen JJ, Chang YC, Su YD, Wang WH. 2013. Secondary metabolites from the soft coral Sinularia arborea. Marine Drugs 11, 3372–3380. http://dx.doi.org/10.3390/md11093372
Tomono Y, Hirota H, Imahara Y, Fusetani NJ. 1999. Four new steroids from two octocorals. Journal Natural Products 62, 1538–41. http://dx.doi.org/10.1021/np990246l.
Tursch B. 1976. Some recent development in the chemistry of Alcyonaceans. Pure and Applied Chemistry 48, 1–6. http://dx.doi.org/10.1351/pac197648010001
Van Alstyne KL, Wylie CR, Paul VJ. 1994. Antipredator defenses in tropical Pacific soft corals (Coelenterata: Alcyonacea). II. The relative importance of chemical andstructural defenses in three species of Sinularia. Journal of Experimental Marine Biology and Ecology 178(1), 17−34. http://dx.doi.org/10.1016/0022-0981(94)90222-4
Wilkinson CR, Evans E. 1989. Sponge distribution across Davies Reef, Great Barrier Reef, relative tolocation, depth, and water movement. Coral Reefs 8, 1–7. http://dx.doi.org/10.1007/BF00304685
Wylie CR, Paul VJ. 1989. Chemical defences in three species of Sinularia (Coelenterata, Alcyonacea): effects against generalist predators and the butterflyfish Chaetodon unimaculatus Bloch. Journal of Experimental Marine Biology and Ecology 129(2), 141–160. http://dx.doi.org/10.1016/0022-0981(89)90053-1
Zhang CX, Yan SJ, Zhang GW, Lu WG, Su JY, Zeng LM, Gu LQ, Yang XP, Lian YJ. 2005. Cytotoxic diterpenoids from the soft coral Sinularia microclavata. Journal of Natural Products 68, 1087–1089. http://dx.doi.org/10.1021/np058006v.
Zhang GW, Ma XQ, Su JY, Zhang K, Kurihara H, Yao XS, Zeng LM, 2006. Two new bioactive sesquiterpenes from the soft coral Sinularia sp. Natural Product Research 20(7), 659–664. http://dx.doi.org/10.1080/14786410500183233
Zhao HY, Shao CL, Li ZY, Han L, Cao F, Wang CY. 2013. Bioactive pregnane steroids from a South China Sea Gorgonian Carijoa sp. Molecule 18, 3458–3466. http://dx.doi.org/10.3390/molecules18033458
Miguel E. Ayllon, Homero Earl S. Elpa, Ephrime B. Metillo, Mylene M. Uy (2019), Toxicity of crude extracts from soft corals (Anthozoa, Alcyonacea) collected at varying wave exposure sites in Talisayan, Northern Mindanao, Philippines; IJB, V14, N5, May, P116-129
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