Acute and subacute toxicity evaluation of methanolic extract of Hodgsonia heteroclita (Roxb.)
Paper Details
Acute and subacute toxicity evaluation of methanolic extract of Hodgsonia heteroclita (Roxb.)
Abstract
Hodgsonia heteroclita (Roxb.) is a medicinal plant used by the people of Assam as antihyperglycemic agent. However, very less scientific studies have been conducted to confirm its medicinal property. Therefore, present study investigates the toxicological effect of plant on mice model. Acute and sub-acute toxicity was carried out following OECD guidelines. Toxicity study was designed for 14 days period by intraperitoneal injection (i.p.) of different doses plant extract, 50, 100, 500, and 1000 mg/kg body weight (b.w.). Following i.p. injection the animals were continuously observed for 1 h, then frequently for 24 h, and thereafter once per day for 14 days to monitor any kind of behavioral changes. One-tenth of the highest dose of acute toxicity study (i.e., 100 mg/kg b.w.) was taken for sub-acute toxicity study and continued for 14 days with daily administration. On the 15th day, tissue samples, liver, kidney, heart, and spleen were collected and processed for histological and biochemical analysis. All the biochemical studies were done following standard protocols. Acute toxicity study revealed no toxicological effect on albino mice at the tested doses. Sub-acute toxicity study revealed slight changes in the various enzymes and molecules in control and treated mice. However, no significant differences were observed in the treated mice. Similarly, no such observable alterations were noticed in histological architecture of tissue sections of treated mice. The absence of any toxicological effects of extract of H. heteroclita suggests its suitability in medicinal systems. However, further investigations are needs to be done to see the mode of action.
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Ananta Swargiary, Manita Daimari, Partha Pratim Sarma, Rajib Ratan Kashyap (2018), Acute and subacute toxicity evaluation of methanolic extract of Hodgsonia heteroclita (Roxb.); IJB, V12, N6, June, P488-496
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